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Protein

Probable translation initiation factor eIF-2B subunit epsilon

Gene

tif225

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the guanine nucleotide exchange factor for eIF-2.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor eIF-2B subunit epsilon
Alternative name(s):
eIF-2B GDP-GTP exchange factor subunit epsilon
Gene namesi
Name:tif225
ORF Names:SPAC8C9.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC8C9.15c.
PomBaseiSPAC8C9.15c. tif225.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 2B complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 678678Probable translation initiation factor eIF-2B subunit epsilonPRO_0000156077Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei172 – 1721Phosphothreonine1 Publication
Modified residuei500 – 5001Phosphoserine1 Publication
Modified residuei503 – 5031Phosphothreonine1 Publication
Modified residuei506 – 5061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56287.

PTM databases

iPTMnetiP56287.

Interactioni

Subunit structurei

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.By similarity

Protein-protein interaction databases

BioGridi278500. 2 interactions.
MINTiMINT-4691035.

Structurei

Secondary structure

1
678
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 235Combined sources
Turni32 – 354Combined sources
Beta strandi36 – 383Combined sources
Helixi40 – 423Combined sources
Beta strandi43 – 497Combined sources
Helixi50 – 6112Combined sources
Beta strandi65 – 706Combined sources
Helixi74 – 8310Combined sources
Helixi85 – 873Combined sources
Beta strandi92 – 998Combined sources
Helixi108 – 1158Combined sources
Beta strandi123 – 1286Combined sources
Beta strandi130 – 1323Combined sources
Helixi137 – 14711Combined sources
Beta strandi153 – 1619Combined sources
Turni171 – 1733Combined sources
Beta strandi174 – 1796Combined sources
Turni181 – 1833Combined sources
Beta strandi185 – 1917Combined sources
Beta strandi198 – 2003Combined sources
Helixi204 – 2096Combined sources
Beta strandi212 – 22615Combined sources
Helixi229 – 2368Combined sources
Turni243 – 2464Combined sources
Helixi247 – 2526Combined sources
Beta strandi260 – 2667Combined sources
Beta strandi271 – 2733Combined sources
Helixi277 – 28812Combined sources
Helixi290 – 2923Combined sources
Helixi297 – 2993Combined sources
Beta strandi307 – 3093Combined sources
Turni310 – 3123Combined sources
Beta strandi313 – 3153Combined sources
Beta strandi344 – 3474Combined sources
Beta strandi361 – 3644Combined sources
Beta strandi378 – 3814Combined sources
Beta strandi422 – 4287Combined sources
Beta strandi436 – 4383Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99I/J1-678[»]
ProteinModelPortaliP56287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini508 – 674167W2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000216610.
InParanoidiP56287.
KOiK03240.
OMAiINALRCK.
OrthoDBiEOG7NSBB4.
PhylomeDBiP56287.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.90.550.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR001451. Hexapep.
IPR016021. MIF4-like.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
IPR003307. W2_domain.
[Graphical view]
PfamiPF00132. Hexapep. 2 hits.
PF00483. NTP_transferase. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPSKGLNGK LEKPKHALQA IVLSDSYNYR FRPLTLDKPR CLLPLANTPL
60 70 80 90 100
IEYTFEFLAL AGVQEVYVFC CAHAGQIREY IEKSKWNLPS SPFSVNTIVS
110 120 130 140 150
RESLSVGDAL RELDSKQLIT SDFILVSGDV VSNVPLNEVL KEHRKRREDD
160 170 180 190 200
KNAIMTMVVR EASPFHRTRA RTESSVFVID KKTSQCVHYQ ANERGKHYVS
210 220 230 240 250
MDPEIFNEHE ELEVRNDLID CQIDICSNDV PALFTENFDY QDIRKDFVYG
260 270 280 290 300
VLTSDLLGKK IHCHVAKENY AARVRSLQTY DAISKDVLSR WVYPFVPDSN
310 320 330 340 350
LLNQTFSYQR HQIYKEEDVV LARSCIIKAR TLIGAYTKVG DASVVANTII
360 370 380 390 400
GRNCTIGSNC SIDSAFLWED VVIGDNCRIG KAILANSVKI GNNCSIEDGA
410 420 430 440 450
IVAAGVVIGD NTIIEKNKRL TTFESHSQGT LNDPSLVGIG GRGQEYHAEE
460 470 480 490 500
DSDDEGEFME ASGLIESTNE LHLSDSESSE TSSSSEEDME FIPFSARRDS
510 520 530 540 550
ANTINSEDFD EGDFNKEAQQ SLERAFEENH QIDIAALELN TLRMAMNANY
560 570 580 590 600
HEVRSAIVLA LLRRIMHLDV SPKEALAKVM TRWGPLLAKL TFSHEEQVDN
610 620 630 640 650
VLTLQKYCVR LSMTRHFLQL LGYFYQLEIA EENAIQEWYS DPRSSEGELA
660 670
ALRDAGGKQF VDWLNTAESE SESEEGSE
Length:678
Mass (Da):76,326
Last modified:July 15, 1998 - v1
Checksum:iD399547CBD82C4BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16302.1.
PIRiT39151.
RefSeqiNP_594285.1. NM_001019708.2.

Genome annotation databases

EnsemblFungiiSPAC8C9.15c.1; SPAC8C9.15c.1:pep; SPAC8C9.15c.
GeneIDi2542017.
KEGGispo:SPAC8C9.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16302.1.
PIRiT39151.
RefSeqiNP_594285.1. NM_001019708.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99I/J1-678[»]
ProteinModelPortaliP56287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278500. 2 interactions.
MINTiMINT-4691035.

PTM databases

iPTMnetiP56287.

Proteomic databases

MaxQBiP56287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC8C9.15c.1; SPAC8C9.15c.1:pep; SPAC8C9.15c.
GeneIDi2542017.
KEGGispo:SPAC8C9.15c.

Organism-specific databases

EuPathDBiFungiDB:SPAC8C9.15c.
PomBaseiSPAC8C9.15c. tif225.

Phylogenomic databases

HOGENOMiHOG000216610.
InParanoidiP56287.
KOiK03240.
OMAiINALRCK.
OrthoDBiEOG7NSBB4.
PhylomeDBiP56287.

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiP56287.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.90.550.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR001451. Hexapep.
IPR016021. MIF4-like.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
IPR003307. W2_domain.
[Graphical view]
PfamiPF00132. Hexapep. 2 hits.
PF00483. NTP_transferase. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-172; SER-500; THR-503 AND SER-506, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEI2BE_SCHPO
AccessioniPrimary (citable) accession number: P56287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.