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Protein

Probable translation initiation factor eIF-2B subunit epsilon

Gene

tif225

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the guanine nucleotide exchange factor for eIF-2.By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: PomBase
  • nucleotidyltransferase activity Source: InterPro
  • translation initiation factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor eIF-2B subunit epsilon
Alternative name(s):
eIF-2B GDP-GTP exchange factor subunit epsilon
Gene namesi
Name:tif225
ORF Names:SPAC8C9.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC8C9.15c.
PomBaseiSPAC8C9.15c. tif225.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 2B complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001560771 – 678Probable translation initiation factor eIF-2B subunit epsilonAdd BLAST678

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei172Phosphothreonine1 Publication1
Modified residuei500Phosphoserine1 Publication1
Modified residuei503Phosphothreonine1 Publication1
Modified residuei506Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56287.
PRIDEiP56287.

PTM databases

iPTMnetiP56287.

Interactioni

Subunit structurei

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.By similarity

Protein-protein interaction databases

BioGridi278500. 2 interactors.
DIPiDIP-61962N.
MINTiMINT-4691035.

Structurei

Secondary structure

1678
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 23Combined sources5
Turni32 – 35Combined sources4
Beta strandi36 – 38Combined sources3
Helixi40 – 42Combined sources3
Beta strandi43 – 49Combined sources7
Helixi50 – 61Combined sources12
Beta strandi65 – 70Combined sources6
Helixi74 – 83Combined sources10
Helixi85 – 87Combined sources3
Beta strandi92 – 99Combined sources8
Helixi108 – 115Combined sources8
Beta strandi123 – 128Combined sources6
Beta strandi130 – 132Combined sources3
Helixi137 – 147Combined sources11
Beta strandi153 – 161Combined sources9
Turni171 – 173Combined sources3
Beta strandi174 – 179Combined sources6
Turni181 – 183Combined sources3
Beta strandi185 – 191Combined sources7
Beta strandi198 – 200Combined sources3
Helixi204 – 209Combined sources6
Beta strandi212 – 226Combined sources15
Helixi229 – 236Combined sources8
Turni243 – 246Combined sources4
Helixi247 – 252Combined sources6
Beta strandi260 – 266Combined sources7
Beta strandi271 – 273Combined sources3
Helixi277 – 288Combined sources12
Helixi290 – 292Combined sources3
Helixi297 – 299Combined sources3
Beta strandi307 – 309Combined sources3
Turni310 – 312Combined sources3
Beta strandi313 – 315Combined sources3
Beta strandi344 – 347Combined sources4
Beta strandi361 – 364Combined sources4
Beta strandi378 – 381Combined sources4
Beta strandi422 – 428Combined sources7
Beta strandi436 – 438Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99I/J1-678[»]
ProteinModelPortaliP56287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini508 – 674W2PROSITE-ProRule annotationAdd BLAST167

Sequence similaritiesi

Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000216610.
InParanoidiP56287.
KOiK03240.
OMAiCIPKMGD.
OrthoDBiEOG092C0S7J.
PhylomeDBiP56287.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.90.550.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR001451. Hexapep.
IPR016021. MIF4-like.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
IPR003307. W2_domain.
[Graphical view]
PfamiPF00132. Hexapep. 2 hits.
PF00483. NTP_transferase. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPSKGLNGK LEKPKHALQA IVLSDSYNYR FRPLTLDKPR CLLPLANTPL
60 70 80 90 100
IEYTFEFLAL AGVQEVYVFC CAHAGQIREY IEKSKWNLPS SPFSVNTIVS
110 120 130 140 150
RESLSVGDAL RELDSKQLIT SDFILVSGDV VSNVPLNEVL KEHRKRREDD
160 170 180 190 200
KNAIMTMVVR EASPFHRTRA RTESSVFVID KKTSQCVHYQ ANERGKHYVS
210 220 230 240 250
MDPEIFNEHE ELEVRNDLID CQIDICSNDV PALFTENFDY QDIRKDFVYG
260 270 280 290 300
VLTSDLLGKK IHCHVAKENY AARVRSLQTY DAISKDVLSR WVYPFVPDSN
310 320 330 340 350
LLNQTFSYQR HQIYKEEDVV LARSCIIKAR TLIGAYTKVG DASVVANTII
360 370 380 390 400
GRNCTIGSNC SIDSAFLWED VVIGDNCRIG KAILANSVKI GNNCSIEDGA
410 420 430 440 450
IVAAGVVIGD NTIIEKNKRL TTFESHSQGT LNDPSLVGIG GRGQEYHAEE
460 470 480 490 500
DSDDEGEFME ASGLIESTNE LHLSDSESSE TSSSSEEDME FIPFSARRDS
510 520 530 540 550
ANTINSEDFD EGDFNKEAQQ SLERAFEENH QIDIAALELN TLRMAMNANY
560 570 580 590 600
HEVRSAIVLA LLRRIMHLDV SPKEALAKVM TRWGPLLAKL TFSHEEQVDN
610 620 630 640 650
VLTLQKYCVR LSMTRHFLQL LGYFYQLEIA EENAIQEWYS DPRSSEGELA
660 670
ALRDAGGKQF VDWLNTAESE SESEEGSE
Length:678
Mass (Da):76,326
Last modified:July 15, 1998 - v1
Checksum:iD399547CBD82C4BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16302.1.
PIRiT39151.
RefSeqiNP_594285.1. NM_001019708.2.

Genome annotation databases

EnsemblFungiiSPAC8C9.15c.1; SPAC8C9.15c.1:pep; SPAC8C9.15c.
GeneIDi2542017.
KEGGispo:SPAC8C9.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16302.1.
PIRiT39151.
RefSeqiNP_594285.1. NM_001019708.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99I/J1-678[»]
ProteinModelPortaliP56287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278500. 2 interactors.
DIPiDIP-61962N.
MINTiMINT-4691035.

PTM databases

iPTMnetiP56287.

Proteomic databases

MaxQBiP56287.
PRIDEiP56287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC8C9.15c.1; SPAC8C9.15c.1:pep; SPAC8C9.15c.
GeneIDi2542017.
KEGGispo:SPAC8C9.15c.

Organism-specific databases

EuPathDBiFungiDB:SPAC8C9.15c.
PomBaseiSPAC8C9.15c. tif225.

Phylogenomic databases

HOGENOMiHOG000216610.
InParanoidiP56287.
KOiK03240.
OMAiCIPKMGD.
OrthoDBiEOG092C0S7J.
PhylomeDBiP56287.

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiP56287.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.90.550.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR001451. Hexapep.
IPR016021. MIF4-like.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
IPR003307. W2_domain.
[Graphical view]
PfamiPF00132. Hexapep. 2 hits.
PF00483. NTP_transferase. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEI2BE_SCHPO
AccessioniPrimary (citable) accession number: P56287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: October 5, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.