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Protein

Eukaryotic translation initiation factor 2 subunit alpha

Gene

tif211

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
  • mitotic G1 DNA damage checkpoint Source: PomBase
  • regulation of cytoplasmic translational initiation in response to stress Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-381042. PERK regulates gene expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.
R-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit alpha
Short name:
eIF-2-alpha
Gene namesi
Name:tif211
ORF Names:SPAC3G9.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3G9.09c.
PomBaseiSPAC3G9.09c. tif211.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Eukaryotic translation initiation factor 2 subunit alphaPRO_0000137388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei179 – 1791Phosphothreonine1 Publication
Modified residuei273 – 2731Phosphoserine1 Publication
Modified residuei295 – 2951Phosphoserine1 Publication
Modified residuei303 – 3031Phosphoserine1 Publication
Modified residuei305 – 3051Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56286.

PTM databases

iPTMnetiP56286.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.

Protein-protein interaction databases

BioGridi279875. 4 interactions.
MINTiMINT-4691022.

Structurei

3D structure databases

ProteinModelPortaliP56286.
SMRiP56286. Positions 3-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 8872S1 motifPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000199476.
InParanoidiP56286.
KOiK03237.
OMAiKWLQFVA.
OrthoDBiEOG092C3ENF.
PhylomeDBiP56286.

Family and domain databases

Gene3Di1.10.150.190. 1 hit.
2.40.50.140. 1 hit.
3.30.70.1130. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR024055. TIF2_asu_C.
IPR024054. TIF2_asu_middle.
IPR011488. TIF_2_asu.
[Graphical view]
PfamiPF07541. EIF_2_alpha. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF110993. SSF110993. 1 hit.
SSF116742. SSF116742. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTSCRMYE NRFPEVDELV VVNVRQIQEM GAYVKLLEYD NIEGMVLLSE
60 70 80 90 100
LSRRRIRSVQ KHIRVGRNEV VVVLRVDKEK GYIDLSKRRV SPEDVVKCEE
110 120 130 140 150
RFNKSKAVHS IMRHIAEKHN VPLETMYTTI GWPLYRKYGH AYDAFKLAIS
160 170 180 190 200
NPDHVFEGLE PPKSGVINDL LAQISRRLTP QPIKIRADVE VTCFGYEGIN
210 220 230 240 250
AIKAALKAAE DVHTEEVPIK VKLVAPPLYV LLTNALDKSL GLKKLEEAIG
260 270 280 290 300
AIEKSITASN GTCTVKMKPK AVSETDELEL ADLMKKFEKE NAEISGDEED

DQSGSE
Length:306
Mass (Da):34,526
Last modified:July 15, 1998 - v1
Checksum:i6C967FF2C3BD0D1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA15918.1.
PIRiT11645.
RefSeqiNP_594081.1. NM_001019504.2.

Genome annotation databases

EnsemblFungiiSPAC3G9.09c.1; SPAC3G9.09c.1:pep; SPAC3G9.09c.
GeneIDi2543455.
KEGGispo:SPAC3G9.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA15918.1.
PIRiT11645.
RefSeqiNP_594081.1. NM_001019504.2.

3D structure databases

ProteinModelPortaliP56286.
SMRiP56286. Positions 3-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279875. 4 interactions.
MINTiMINT-4691022.

PTM databases

iPTMnetiP56286.

Proteomic databases

MaxQBiP56286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3G9.09c.1; SPAC3G9.09c.1:pep; SPAC3G9.09c.
GeneIDi2543455.
KEGGispo:SPAC3G9.09c.

Organism-specific databases

EuPathDBiFungiDB:SPAC3G9.09c.
PomBaseiSPAC3G9.09c. tif211.

Phylogenomic databases

HOGENOMiHOG000199476.
InParanoidiP56286.
KOiK03237.
OMAiKWLQFVA.
OrthoDBiEOG092C3ENF.
PhylomeDBiP56286.

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-381042. PERK regulates gene expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.
R-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiP56286.

Family and domain databases

Gene3Di1.10.150.190. 1 hit.
2.40.50.140. 1 hit.
3.30.70.1130. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR024055. TIF2_asu_C.
IPR024054. TIF2_asu_middle.
IPR011488. TIF_2_asu.
[Graphical view]
PfamiPF07541. EIF_2_alpha. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF110993. SSF110993. 1 hit.
SSF116742. SSF116742. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2A_SCHPO
AccessioniPrimary (citable) accession number: P56286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.