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Protein

Eukaryotic translation initiation factor 2 subunit alpha

Gene

tif211

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
  • mitotic G1 DNA damage checkpoint Source: PomBase

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SPO-72649 Translation initiation complex formation
R-SPO-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SPO-72702 Ribosomal scanning and start codon recognition
R-SPO-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit alpha
Short name:
eIF-2-alpha
Gene namesi
Name:tif211
ORF Names:SPAC3G9.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3G9.09c
PomBaseiSPAC3G9.09c tif211

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001373881 – 306Eukaryotic translation initiation factor 2 subunit alphaAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineBy similarity1
Modified residuei179Phosphothreonine1 Publication1
Modified residuei273Phosphoserine1 Publication1
Modified residuei295Phosphoserine1 Publication1
Modified residuei303Phosphoserine1 Publication1
Modified residuei305Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56286
PaxDbiP56286
PRIDEiP56286

PTM databases

iPTMnetiP56286

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2798754 interactors.
DIPiDIP-61963N
IntActiP56286 3 interactors.
STRINGi4896.SPAC3G9.09c.1

Structurei

3D structure databases

ProteinModelPortaliP56286
SMRiP56286
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 88S1 motifPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated

Phylogenomic databases

HOGENOMiHOG000199476
InParanoidiP56286
KOiK03237
OMAiKDVNEHQ
OrthoDBiEOG092C3ENF
PhylomeDBiP56286

Family and domain databases

Gene3Di1.10.150.1901 hit
3.30.70.11301 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022967 S1_dom
IPR003029 S1_domain
IPR024055 TIF2_asu_C
IPR024054 TIF2_asu_middle_sf
IPR011488 TIF_2_asu
PANTHERiPTHR10602 PTHR10602, 1 hit
PfamiView protein in Pfam
PF07541 EIF_2_alpha, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SUPFAMiSSF110993 SSF110993, 1 hit
SSF116742 SSF116742, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

P56286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTSCRMYE NRFPEVDELV VVNVRQIQEM GAYVKLLEYD NIEGMVLLSE
60 70 80 90 100
LSRRRIRSVQ KHIRVGRNEV VVVLRVDKEK GYIDLSKRRV SPEDVVKCEE
110 120 130 140 150
RFNKSKAVHS IMRHIAEKHN VPLETMYTTI GWPLYRKYGH AYDAFKLAIS
160 170 180 190 200
NPDHVFEGLE PPKSGVINDL LAQISRRLTP QPIKIRADVE VTCFGYEGIN
210 220 230 240 250
AIKAALKAAE DVHTEEVPIK VKLVAPPLYV LLTNALDKSL GLKKLEEAIG
260 270 280 290 300
AIEKSITASN GTCTVKMKPK AVSETDELEL ADLMKKFEKE NAEISGDEED

DQSGSE
Length:306
Mass (Da):34,526
Last modified:July 15, 1998 - v1
Checksum:i6C967FF2C3BD0D1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAA15918.1
PIRiT11645
RefSeqiNP_594081.1, NM_001019504.2

Genome annotation databases

EnsemblFungiiSPAC3G9.09c.1; SPAC3G9.09c.1:pep; SPAC3G9.09c
GeneIDi2543455
KEGGispo:SPAC3G9.09c

Similar proteinsi

Entry informationi

Entry nameiIF2A_SCHPO
AccessioniPrimary (citable) accession number: P56286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome