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P56282

- DPOE2_HUMAN

UniProt

P56282 - DPOE2_HUMAN

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Protein

DNA polymerase epsilon subunit 2

Gene
POLE2, DPE2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in DNA repair and in chromosomal DNA replication.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: ProtInc

GO - Biological processi

  1. DNA repair Source: Reactome
  2. DNA replication Source: ProtInc
  3. DNA replication initiation Source: Reactome
  4. G1/S transition of mitotic cell cycle Source: Reactome
  5. mitotic cell cycle Source: Reactome
  6. nucleotide-excision repair Source: Reactome
  7. nucleotide-excision repair, DNA gap filling Source: Reactome
  8. telomere maintenance Source: Reactome
  9. telomere maintenance via recombination Source: Reactome
  10. telomere maintenance via semi-conservative replication Source: Reactome
  11. transcription-coupled nucleotide-excision repair Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_1095. Activation of the pre-replicative complex.
REACT_1993. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_2244. DNA replication initiation.
REACT_378. Repair synthesis of patch ~27-30 bases long by DNA polymerase.
REACT_7993. Telomere C-strand synthesis initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 2 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 2
DNA polymerase epsilon subunit B
Gene namesi
Name:POLE2
Synonyms:DPE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:9178. POLE2.

Subcellular locationi

GO - Cellular componenti

  1. epsilon DNA polymerase complex Source: UniProtKB
  2. intracellular membrane-bounded organelle Source: HPA
  3. nucleoplasm Source: Reactome
  4. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA278.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527DNA polymerase epsilon subunit 2PRO_0000071562Add
BLAST

Proteomic databases

MaxQBiP56282.
PaxDbiP56282.
PRIDEiP56282.

PTM databases

PhosphoSiteiP56282.

Expressioni

Gene expression databases

ArrayExpressiP56282.
BgeeiP56282.
CleanExiHS_POLE2.
GenevestigatoriP56282.

Organism-specific databases

HPAiHPA027555.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4.1 Publication

Protein-protein interaction databases

BioGridi111423. 12 interactions.
IntActiP56282. 7 interactions.
MINTiMINT-1377461.
STRINGi9606.ENSP00000216367.

Structurei

Secondary structure

1
527
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1614
Helixi23 – 3210
Turni33 – 353
Turni38 – 403
Helixi41 – 5212
Beta strandi58 – 625
Helixi64 – 7310

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V6ZNMR-M1-73[»]
ProteinModelPortaliP56282.
SMRiP56282. Positions 1-75.

Miscellaneous databases

EvolutionaryTraceiP56282.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG311342.
HOGENOMiHOG000265254.
HOVERGENiHBG061290.
InParanoidiP56282.
KOiK02325.
OMAiILQQRTH.
OrthoDBiEOG725DH7.
PhylomeDBiP56282.
TreeFamiTF103007.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view]
PfamiPF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view]
PIRSFiPIRSF000799. DNA_pol_eps_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P56282-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN    50
AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN 100
SERKKFLPLL MTNHPAPNLF GTPRDKAEMF RERYTILHQR THRHELFTPP 150
VIGSHPDESG SKFQLKTIET LLGSTTKIGD AIVLGMITQL KEGKFFLEDP 200
TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF GFPPTEPSST 250
TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV 300
LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC 350
EYPDIHQSSR FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT 400
NPCRIQYCTQ EITVFREDLV NKMCRNCVRF PSSNLAIPNH FVKTILSQGH 450
LTPLPLYVCP VYWAYDYALR VYPVPDLLVI ADKYDPFTTT NTECLCINPG 500
SFPRSGFSFK VFYPSNKTVE DSKLQGF 527
Length:527
Mass (Da):59,537
Last modified:December 15, 1998 - v2
Checksum:iAFA7AF7C2C0BFF15
GO
Isoform 2 (identifier: P56282-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-108: Missing.

Note: No experimental confirmation available.

Show »
Length:501
Mass (Da):56,418
Checksum:iECB4BFD1E2FDAE1C
GO
Isoform 3 (identifier: P56282-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-527: GSFPRSGFSFKVFYPSNKTVEDSKLQGF → VRM

Show »
Length:502
Mass (Da):56,784
Checksum:i0F3391E93AB14483
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti84 – 841H → P.1 Publication
Corresponds to variant rs34857719 [ dbSNP | Ensembl ].
VAR_044379
Natural varianti456 – 4561L → V.
Corresponds to variant rs34574266 [ dbSNP | Ensembl ].
VAR_044380
Natural varianti514 – 5141P → L.1 Publication
Corresponds to variant rs45515094 [ dbSNP | Ensembl ].
VAR_044381

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei83 – 10826Missing in isoform 2. VSP_042551Add
BLAST
Alternative sequencei500 – 52728GSFPR…KLQGF → VRM in isoform 3. VSP_043796Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111L → P in AAC51920. 1 Publication
Sequence conflicti359 – 3591Missing in AAC51920. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF025840 mRNA. Translation: AAC51920.1.
AF036899 mRNA. Translation: AAC39610.1.
AF387034
, AF387021, AF387022, AF387023, AF387024, AF387025, AF387026, AF387027, AF387028, AF387029, AF387030, AF387031, AF387032, AF387033 Genomic DNA. Translation: AAK72254.1.
EF506887 Genomic DNA. Translation: ABO43040.1.
AK293163 mRNA. Translation: BAG56707.1.
AL139099 Genomic DNA. No translation available.
AL591767 Genomic DNA. No translation available.
BC112962 mRNA. Translation: AAI12963.1.
BC126218 mRNA. Translation: AAI26219.1.
BC126220 mRNA. Translation: AAI26221.1.
CCDSiCCDS32073.1. [P56282-1]
CCDS55914.1. [P56282-2]
CCDS55915.1. [P56282-3]
RefSeqiNP_001184259.1. NM_001197330.1. [P56282-2]
NP_001184260.1. NM_001197331.1. [P56282-3]
NP_002683.2. NM_002692.3. [P56282-1]
UniGeneiHs.162777.

Genome annotation databases

EnsembliENST00000216367; ENSP00000216367; ENSG00000100479. [P56282-1]
ENST00000539565; ENSP00000446313; ENSG00000100479. [P56282-2]
ENST00000554396; ENSP00000451621; ENSG00000100479. [P56282-3]
GeneIDi5427.
KEGGihsa:5427.
UCSCiuc001wwu.3. human. [P56282-1]
uc010ano.3. human. [P56282-3]
uc021rsr.1. human. [P56282-2]

Polymorphism databases

DMDMi3915676.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF025840 mRNA. Translation: AAC51920.1 .
AF036899 mRNA. Translation: AAC39610.1 .
AF387034
, AF387021 , AF387022 , AF387023 , AF387024 , AF387025 , AF387026 , AF387027 , AF387028 , AF387029 , AF387030 , AF387031 , AF387032 , AF387033 Genomic DNA. Translation: AAK72254.1 .
EF506887 Genomic DNA. Translation: ABO43040.1 .
AK293163 mRNA. Translation: BAG56707.1 .
AL139099 Genomic DNA. No translation available.
AL591767 Genomic DNA. No translation available.
BC112962 mRNA. Translation: AAI12963.1 .
BC126218 mRNA. Translation: AAI26219.1 .
BC126220 mRNA. Translation: AAI26221.1 .
CCDSi CCDS32073.1. [P56282-1 ]
CCDS55914.1. [P56282-2 ]
CCDS55915.1. [P56282-3 ]
RefSeqi NP_001184259.1. NM_001197330.1. [P56282-2 ]
NP_001184260.1. NM_001197331.1. [P56282-3 ]
NP_002683.2. NM_002692.3. [P56282-1 ]
UniGenei Hs.162777.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2V6Z NMR - M 1-73 [» ]
ProteinModelPortali P56282.
SMRi P56282. Positions 1-75.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111423. 12 interactions.
IntActi P56282. 7 interactions.
MINTi MINT-1377461.
STRINGi 9606.ENSP00000216367.

PTM databases

PhosphoSitei P56282.

Polymorphism databases

DMDMi 3915676.

Proteomic databases

MaxQBi P56282.
PaxDbi P56282.
PRIDEi P56282.

Protocols and materials databases

DNASUi 5427.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000216367 ; ENSP00000216367 ; ENSG00000100479 . [P56282-1 ]
ENST00000539565 ; ENSP00000446313 ; ENSG00000100479 . [P56282-2 ]
ENST00000554396 ; ENSP00000451621 ; ENSG00000100479 . [P56282-3 ]
GeneIDi 5427.
KEGGi hsa:5427.
UCSCi uc001wwu.3. human. [P56282-1 ]
uc010ano.3. human. [P56282-3 ]
uc021rsr.1. human. [P56282-2 ]

Organism-specific databases

CTDi 5427.
GeneCardsi GC14M050110.
HGNCi HGNC:9178. POLE2.
HPAi HPA027555.
MIMi 602670. gene.
neXtProti NX_P56282.
PharmGKBi PA278.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG311342.
HOGENOMi HOG000265254.
HOVERGENi HBG061290.
InParanoidi P56282.
KOi K02325.
OMAi ILQQRTH.
OrthoDBi EOG725DH7.
PhylomeDBi P56282.
TreeFami TF103007.

Enzyme and pathway databases

Reactomei REACT_1095. Activation of the pre-replicative complex.
REACT_1993. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_2244. DNA replication initiation.
REACT_378. Repair synthesis of patch ~27-30 bases long by DNA polymerase.
REACT_7993. Telomere C-strand synthesis initiation.

Miscellaneous databases

EvolutionaryTracei P56282.
GeneWikii POLE2.
GenomeRNAii 5427.
NextBioi 20997.
PROi P56282.
SOURCEi Search...

Gene expression databases

ArrayExpressi P56282.
Bgeei P56282.
CleanExi HS_POLE2.
Genevestigatori P56282.

Family and domain databases

InterProi IPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view ]
Pfami PF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000799. DNA_pol_eps_2. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon."
    Li Y., Asahara H., Patel V.S., Zhou S., Linn S.
    J. Biol. Chem. 272:32337-32344(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon."
    Jokela M., Makiniemi M., Lehtonen S., Szpirer C., Hellman U., Syvaeoja J.E.
    Nucleic Acids Res. 26:730-734(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase varepsilon B-subunit gene POLE2."
    Huang D., Jokela M., Tuusa J., Skog S., Poikonen K., Syvaoja J.E.
    Nucleic Acids Res. 29:2810-2821(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. NIEHS SNPs program
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS PRO-84 AND LEU-514.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Embryonic stem cell.
  8. "Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon."
    Li Y., Pursell Z.F., Linn S.
    J. Biol. Chem. 275:23247-23252(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN EPSILON DNA POLYMERASE COMPLEX.
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiDPOE2_HUMAN
AccessioniPrimary (citable) accession number: P56282
Secondary accession number(s): A0AV55
, A4FU92, A4LBB7, A6NH58, B4DDE6, O43560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: September 3, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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