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Protein

DNA polymerase epsilon subunit 2

Gene

POLE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in DNA repair and in chromosomal DNA replication.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: ProtInc

GO - Biological processi

  • DNA-dependent DNA replication Source: GO_Central
  • DNA repair Source: ProtInc
  • DNA replication Source: ProtInc
  • DNA replication initiation Source: Reactome
  • error-prone translesion synthesis Source: GO_Central
  • G1/S transition of mitotic cell cycle Source: Reactome
  • telomere maintenance via recombination Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS02095-MONOMER.
ReactomeiR-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-174430. Telomere C-strand synthesis initiation.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-68952. DNA replication initiation.
R-HSA-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 2 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 2
DNA polymerase epsilon subunit B
Gene namesi
Name:POLE2
Synonyms:DPE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:9178. POLE2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5427.
OpenTargetsiENSG00000100479.
PharmGKBiPA278.

Chemistry databases

DrugBankiDB00242. Cladribine.

Polymorphism and mutation databases

BioMutaiPOLE2.
DMDMi3915676.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000715621 – 527DNA polymerase epsilon subunit 2Add BLAST527

Proteomic databases

EPDiP56282.
MaxQBiP56282.
PaxDbiP56282.
PeptideAtlasiP56282.
PRIDEiP56282.

PTM databases

iPTMnetiP56282.
PhosphoSitePlusiP56282.

Expressioni

Gene expression databases

BgeeiENSG00000100479.
CleanExiHS_POLE2.
ExpressionAtlasiP56282. baseline and differential.
GenevisibleiP56282. HS.

Organism-specific databases

HPAiHPA027555.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGFG1P525943EBI-713847,EBI-996560
HEL-S-30V9HWB83EBI-713847,EBI-10215395
MAPRE1Q156915EBI-713847,EBI-1004115
RELQ048643EBI-713847,EBI-307352
TBX15Q96SF73EBI-713847,EBI-10191361
TRIM27P143733EBI-713847,EBI-719493

Protein-protein interaction databases

BioGridi111423. 35 interactors.
IntActiP56282. 17 interactors.
MINTiMINT-1377461.
STRINGi9606.ENSP00000216367.

Structurei

Secondary structure

1527
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Helixi23 – 32Combined sources10
Turni33 – 35Combined sources3
Turni38 – 40Combined sources3
Helixi41 – 52Combined sources12
Beta strandi58 – 62Combined sources5
Helixi64 – 73Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6ZNMR-M1-73[»]
ProteinModelPortaliP56282.
SMRiP56282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56282.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3818. Eukaryota.
ENOG410XP7N. LUCA.
GeneTreeiENSGT00390000012435.
HOGENOMiHOG000265254.
HOVERGENiHBG061290.
InParanoidiP56282.
KOiK02325.
OMAiFAFKVYI.
OrthoDBiEOG091G05Q6.
PhylomeDBiP56282.
TreeFamiTF103007.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view]
PfamiPF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view]
PIRSFiPIRSF000799. DNA_pol_eps_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P56282-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN
60 70 80 90 100
AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN
110 120 130 140 150
SERKKFLPLL MTNHPAPNLF GTPRDKAEMF RERYTILHQR THRHELFTPP
160 170 180 190 200
VIGSHPDESG SKFQLKTIET LLGSTTKIGD AIVLGMITQL KEGKFFLEDP
210 220 230 240 250
TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF GFPPTEPSST
260 270 280 290 300
TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV
310 320 330 340 350
LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC
360 370 380 390 400
EYPDIHQSSR FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT
410 420 430 440 450
NPCRIQYCTQ EITVFREDLV NKMCRNCVRF PSSNLAIPNH FVKTILSQGH
460 470 480 490 500
LTPLPLYVCP VYWAYDYALR VYPVPDLLVI ADKYDPFTTT NTECLCINPG
510 520
SFPRSGFSFK VFYPSNKTVE DSKLQGF
Length:527
Mass (Da):59,537
Last modified:December 15, 1998 - v2
Checksum:iAFA7AF7C2C0BFF15
GO
Isoform 2 (identifier: P56282-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-108: Missing.

Note: No experimental confirmation available.
Show »
Length:501
Mass (Da):56,418
Checksum:iECB4BFD1E2FDAE1C
GO
Isoform 3 (identifier: P56282-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-527: GSFPRSGFSFKVFYPSNKTVEDSKLQGF → VRM

Show »
Length:502
Mass (Da):56,784
Checksum:i0F3391E93AB14483
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11L → P in AAC51920 (PubMed:9405441).Curated1
Sequence conflicti359Missing in AAC51920 (PubMed:9405441).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04437984H → P.1 PublicationCorresponds to variant rs34857719dbSNPEnsembl.1
Natural variantiVAR_044380456L → V.Corresponds to variant rs34574266dbSNPEnsembl.1
Natural variantiVAR_044381514P → L.1 PublicationCorresponds to variant rs45515094dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04255183 – 108Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_043796500 – 527GSFPR…KLQGF → VRM in isoform 3. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025840 mRNA. Translation: AAC51920.1.
AF036899 mRNA. Translation: AAC39610.1.
AF387034
, AF387021, AF387022, AF387023, AF387024, AF387025, AF387026, AF387027, AF387028, AF387029, AF387030, AF387031, AF387032, AF387033 Genomic DNA. Translation: AAK72254.1.
EF506887 Genomic DNA. Translation: ABO43040.1.
AK293163 mRNA. Translation: BAG56707.1.
AL139099 Genomic DNA. No translation available.
AL591767 Genomic DNA. No translation available.
BC112962 mRNA. Translation: AAI12963.1.
BC126218 mRNA. Translation: AAI26219.1.
BC126220 mRNA. Translation: AAI26221.1.
CCDSiCCDS32073.1. [P56282-1]
CCDS55914.1. [P56282-2]
CCDS55915.1. [P56282-3]
RefSeqiNP_001184259.1. NM_001197330.1. [P56282-2]
NP_001184260.1. NM_001197331.1. [P56282-3]
NP_002683.2. NM_002692.3. [P56282-1]
UniGeneiHs.162777.

Genome annotation databases

EnsembliENST00000216367; ENSP00000216367; ENSG00000100479. [P56282-1]
ENST00000539565; ENSP00000446313; ENSG00000100479. [P56282-2]
ENST00000554396; ENSP00000451621; ENSG00000100479. [P56282-3]
GeneIDi5427.
KEGGihsa:5427.
UCSCiuc001wwu.4. human. [P56282-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025840 mRNA. Translation: AAC51920.1.
AF036899 mRNA. Translation: AAC39610.1.
AF387034
, AF387021, AF387022, AF387023, AF387024, AF387025, AF387026, AF387027, AF387028, AF387029, AF387030, AF387031, AF387032, AF387033 Genomic DNA. Translation: AAK72254.1.
EF506887 Genomic DNA. Translation: ABO43040.1.
AK293163 mRNA. Translation: BAG56707.1.
AL139099 Genomic DNA. No translation available.
AL591767 Genomic DNA. No translation available.
BC112962 mRNA. Translation: AAI12963.1.
BC126218 mRNA. Translation: AAI26219.1.
BC126220 mRNA. Translation: AAI26221.1.
CCDSiCCDS32073.1. [P56282-1]
CCDS55914.1. [P56282-2]
CCDS55915.1. [P56282-3]
RefSeqiNP_001184259.1. NM_001197330.1. [P56282-2]
NP_001184260.1. NM_001197331.1. [P56282-3]
NP_002683.2. NM_002692.3. [P56282-1]
UniGeneiHs.162777.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6ZNMR-M1-73[»]
ProteinModelPortaliP56282.
SMRiP56282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111423. 35 interactors.
IntActiP56282. 17 interactors.
MINTiMINT-1377461.
STRINGi9606.ENSP00000216367.

Chemistry databases

DrugBankiDB00242. Cladribine.

PTM databases

iPTMnetiP56282.
PhosphoSitePlusiP56282.

Polymorphism and mutation databases

BioMutaiPOLE2.
DMDMi3915676.

Proteomic databases

EPDiP56282.
MaxQBiP56282.
PaxDbiP56282.
PeptideAtlasiP56282.
PRIDEiP56282.

Protocols and materials databases

DNASUi5427.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216367; ENSP00000216367; ENSG00000100479. [P56282-1]
ENST00000539565; ENSP00000446313; ENSG00000100479. [P56282-2]
ENST00000554396; ENSP00000451621; ENSG00000100479. [P56282-3]
GeneIDi5427.
KEGGihsa:5427.
UCSCiuc001wwu.4. human. [P56282-1]

Organism-specific databases

CTDi5427.
DisGeNETi5427.
GeneCardsiPOLE2.
HGNCiHGNC:9178. POLE2.
HPAiHPA027555.
MIMi602670. gene.
neXtProtiNX_P56282.
OpenTargetsiENSG00000100479.
PharmGKBiPA278.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3818. Eukaryota.
ENOG410XP7N. LUCA.
GeneTreeiENSGT00390000012435.
HOGENOMiHOG000265254.
HOVERGENiHBG061290.
InParanoidiP56282.
KOiK02325.
OMAiFAFKVYI.
OrthoDBiEOG091G05Q6.
PhylomeDBiP56282.
TreeFamiTF103007.

Enzyme and pathway databases

BioCyciZFISH:HS02095-MONOMER.
ReactomeiR-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-174430. Telomere C-strand synthesis initiation.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-68952. DNA replication initiation.
R-HSA-68962. Activation of the pre-replicative complex.

Miscellaneous databases

EvolutionaryTraceiP56282.
GeneWikiiPOLE2.
GenomeRNAii5427.
PROiP56282.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100479.
CleanExiHS_POLE2.
ExpressionAtlasiP56282. baseline and differential.
GenevisibleiP56282. HS.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view]
PfamiPF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view]
PIRSFiPIRSF000799. DNA_pol_eps_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE2_HUMAN
AccessioniPrimary (citable) accession number: P56282
Secondary accession number(s): A0AV55
, A4FU92, A4LBB7, A6NH58, B4DDE6, O43560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.