P56282 (DPOE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase epsilon subunit 2
EC=2.7.7.7

Alternative name(s):
DNA polymerase II subunit 2
DNA polymerase epsilon subunit B

Gene names

Name:	POLE2
Synonyms:	DPE2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in DNA repair and in chromosomal DNA replication.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Ref.8
Subcellular location	Nucleus.
Miscellaneous	In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Sequence similarities	Belongs to the DNA polymerase epsilon subunit B family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Traceable author statement. Source: Reactome G1/S transition of mitotic cell cycle Traceable author statement. Source: Reactome M/G1 transition of mitotic cell cycle Traceable author statement. Source: Reactome S phase of mitotic cell cycle Traceable author statement. Source: Reactome nucleotide-excision repair, DNA gap filling Traceable author statement. Source: Reactome telomere maintenance via recombination Traceable author statement. Source: Reactome telomere maintenance via semi-conservative replication Traceable author statement. Source: Reactome transcription-coupled nucleotide-excision repair Traceable author statement. Source: Reactome
Cellular_component	epsilon DNA polymerase complex Inferred from direct assay Ref.8. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P56282-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P56282-2)

The sequence of this isoform differs from the canonical sequence as follows:
     83-108: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: P56282-3)

The sequence of this isoform differs from the canonical sequence as follows:
     500-527: GSFPRSGFSFKVFYPSNKTVEDSKLQGF → VRM

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 527

527

DNA polymerase epsilon subunit 2

PRO_0000071562

Natural variations

Alternative sequence

83 – 108

26

Missing in isoform 2.

VSP_042551

Alternative sequence

500 – 527

28

GSFPR…KLQGF → VRM in isoform 3.

VSP_043796

Natural variant

84

1

H → P. Ref.4
Corresponds to variant rs34857719 [ dbSNP | Ensembl ].

VAR_044379

Natural variant

456

1

L → V.
Corresponds to variant rs34574266 [ dbSNP | Ensembl ].

VAR_044380

Natural variant

514

1

P → L. Ref.4
Corresponds to variant rs45515094 [ dbSNP | Ensembl ].

VAR_044381

Experimental info

Sequence conflict

11

1

L → P in AAC51920. Ref.1

Sequence conflict

359

1

Missing in AAC51920. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

527

Helix Strand Turn

Details...

Helix

3 – 16

14

Helix

23 – 32

10

Turn

33 – 35

3

Turn

38 – 40

3

Helix

41 – 52

12

Beta strand

58 – 62

5

Helix

64 – 73

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: AFA7AF7C2C0BFF15	FASTA	527	59,537
	10 20 30 40 50 60 MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN 70 80 90 100 110 120 MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN SERKKFLPLL MTNHPAPNLF 130 140 150 160 170 180 GTPRDKAEMF RERYTILHQR THRHELFTPP VIGSHPDESG SKFQLKTIET LLGSTTKIGD 190 200 210 220 230 240 AIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF 250 260 270 280 290 300 GFPPTEPSST TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV 310 320 330 340 350 360 LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC EYPDIHQSSR 370 380 390 400 410 420 FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT NPCRIQYCTQ EITVFREDLV 430 440 450 460 470 480 NKMCRNCVRF PSSNLAIPNH FVKTILSQGH LTPLPLYVCP VYWAYDYALR VYPVPDLLVI 490 500 510 520 ADKYDPFTTT NTECLCINPG SFPRSGFSFK VFYPSNKTVE DSKLQGF
	Isoform 2 [UniParc]. Checksum: ECB4BFD1E2FDAE1C	FASTA	501	56,418
	10 20 30 40 50 60 MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN 70 80 90 100 110 120 MIERSVVEAA VQECSQSVDE TILLMTNHPA PNLFGTPRDK AEMFRERYTI LHQRTHRHEL 130 140 150 160 170 180 FTPPVIGSHP DESGSKFQLK TIETLLGSTT KIGDAIVLGM ITQLKEGKFF LEDPTGTVQL 190 200 210 220 230 240 DLSKAQFHSG LYTEACFVLA EGWFEDQVFH VNAFGFPPTE PSSTTRAYYG NINFFGGPSN 250 260 270 280 290 300 TSVKTSAKLK QLEEENKDAM FVFLSDVWLD QVEVLEKLRI MFAGYSPAPP TCFILCGNFS 310 320 330 340 350 360 SAPYGKNQVQ ALKDSLKTLA DIICEYPDIH QSSRFVFVPG PEDPGFGSIL PRPPLAESIT 370 380 390 400 410 420 NEFRQRVPFS VFTTNPCRIQ YCTQEITVFR EDLVNKMCRN CVRFPSSNLA IPNHFVKTIL 430 440 450 460 470 480 SQGHLTPLPL YVCPVYWAYD YALRVYPVPD LLVIADKYDP FTTTNTECLC INPGSFPRSG 490 500 FSFKVFYPSN KTVEDSKLQG F
	Isoform 3 [UniParc]. Checksum: 0F3391E93AB14483	FASTA	502	56,784
	10 20 30 40 50 60 MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN 70 80 90 100 110 120 MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN SERKKFLPLL MTNHPAPNLF 130 140 150 160 170 180 GTPRDKAEMF RERYTILHQR THRHELFTPP VIGSHPDESG SKFQLKTIET LLGSTTKIGD 190 200 210 220 230 240 AIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF 250 260 270 280 290 300 GFPPTEPSST TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV 310 320 330 340 350 360 LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC EYPDIHQSSR 370 380 390 400 410 420 FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT NPCRIQYCTQ EITVFREDLV 430 440 450 460 470 480 NKMCRNCVRF PSSNLAIPNH FVKTILSQGH LTPLPLYVCP VYWAYDYALR VYPVPDLLVI 490 500 ADKYDPFTTT NTECLCINPV RM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon." Li Y., Asahara H., Patel V.S., Zhou S., Linn S. J. Biol. Chem. 272:32337-32344(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon." Jokela M., Makiniemi M., Lehtonen S., Szpirer C., Hellman U., Syvaeoja J.E. Nucleic Acids Res. 26:730-734(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase varepsilon B-subunit gene POLE2." Huang D., Jokela M., Tuusa J., Skog S., Poikonen K., Syvaoja J.E. Nucleic Acids Res. 29:2810-2821(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	NIEHS SNPs program Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS PRO-84 AND LEU-514.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Embryonic stem cell.
[8]	"Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon." Li Y., Pursell Z.F., Linn S. J. Biol. Chem. 275:23247-23252(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN EPSILON DNA POLYMERASE COMPLEX. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF025840 mRNA. Translation: AAC51920.1.
AF036899 mRNA. Translation: AAC39610.1.
AF387034 , AF387021, AF387022, AF387023, AF387024, AF387025, AF387026, AF387027, AF387028, AF387029, AF387030, AF387031, AF387032, AF387033 Genomic DNA. Translation: AAK72254.1.
EF506887 Genomic DNA. Translation: ABO43040.1.
AK293163 mRNA. Translation: BAG56707.1.
AL139099 Genomic DNA. No translation available.
AL591767 Genomic DNA. No translation available.
BC112962 mRNA. Translation: AAI12963.1.
BC126218 mRNA. Translation: AAI26219.1.
BC126220 mRNA. Translation: AAI26221.1.

IPI

IPI00008829.
IPI00980278.

RefSeq

NP_001184259.1. NM_001197330.1.
NP_001184260.1. NM_001197331.1.
NP_002683.2. NM_002692.3.

UniGene

Hs.162777.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V6Z	NMR	-	M	1-73	[»]

ProteinModelPortal

P56282.

ModBase

Search...

Protein-protein interaction databases

IntAct

P56282. 7 interactions.

MINT

MINT-1377461.

STRING

9606.ENSP00000216367.

PTM databases

PhosphoSite

P56282.

Polymorphism databases

DMDM

3915676.

Proteomic databases

PaxDb

P56282.

PRIDE

P56282.

Protocols and materials databases

DNASU

5427.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000216367; ENSP00000216367; ENSG00000100479.
ENST00000539565; ENSP00000446313; ENSG00000100479.
ENST00000554396; ENSP00000451621; ENSG00000100479.

GeneID

5427.

KEGG

hsa:5427.

UCSC

uc001wwu.3. human.

Organism-specific databases

CTD

5427.

GeneCards

GC14M050110.

HGNC

HGNC:9178. POLE2.

HPA

HPA027555.

MIM

602670. gene.

neXtProt

NX_P56282.

PharmGKB

PA278.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG311342.

HOGENOM

HOG000265254.

HOVERGEN

HBG061290.

InParanoid

P56282.

KO

K02325.

OMA

PNHFVKT.

OrthoDB

EOG4Z8XW9.

Enzyme and pathway databases

Reactome

REACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.
REACT_216. DNA Repair.
REACT_383. DNA Replication.

Gene expression databases

ArrayExpress

P56282.

Bgee

P56282.

CleanEx

HS_POLE2.

Genevestigator

P56282.

GermOnline

ENSG00000100479. Homo sapiens.

Family and domain databases

InterPro

IPR007185. DNA_pol_alpha/epsilon_bsu.
IPR016266. DNA_pol_e_bsu.
IPR024639. DNA_pol_e_bsu_N.
[Graphical view]

Pfam

PF04042. DNA_pol_E_B. 1 hit.
PF12213. Dpoe2NT. 1 hit.
[Graphical view]

PIRSF

PIRSF000799. DNA_pol_eps_2. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P56282.

GenomeRNAi

5427.

NextBio

20997.

SOURCE

Search...

Entry information

Entry name

DPOE2_HUMAN

Accession

Primary (citable) accession number: P56282
Secondary accession number(s): A0AV55 , A4FU92, A4LBB7, A6NH58, B4DDE6, O43560

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.