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P56273

- MDM2_XENLA

UniProt

P56273 - MDM2_XENLA

Protein

E3 ubiquitin-protein ligase Mdm2

Gene

mdm2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri290 – 31930RanBP2-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri420 – 46142RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. negative regulation of apoptotic process Source: InterPro
    2. negative regulation of cell cycle arrest Source: InterPro
    3. negative regulation of transcription from RNA polymerase II promoter Source: InterPro
    4. protein ubiquitination Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase Mdm2 (EC:6.3.2.-)
    Alternative name(s):
    Double minute 2 protein
    Short name:
    Xdm2
    p53-binding protein Mdm2
    Gene namesi
    Name:mdm2
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6255114. mdm2.

    Subcellular locationi

    Nucleusnucleoplasm By similarity. Cytoplasm By similarity. Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: UniProtKB-SubCell
    3. nucleoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473E3 ubiquitin-protein ligase Mdm2PRO_0000157334Add
    BLAST

    Expressioni

    Developmental stagei

    Expression increases from oocyte stage I/II to reach its maximum in oocyte stage v/vi in unfertilized eggs, and then progressively decreases to become undetectable at the gastrula stage.

    Interactioni

    Protein-protein interaction databases

    IntActiP56273. 1 interaction.

    Structurei

    Secondary structure

    1
    473
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi24 – 263
    Helixi28 – 369
    Helixi46 – 5914
    Beta strandi65 – 673
    Beta strandi70 – 723
    Helixi78 – 825
    Beta strandi85 – 884
    Helixi92 – 1009
    Beta strandi103 – 1064

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TTVNMR-A13-119[»]
    1YCQX-ray2.30A13-119[»]
    4IPFX-ray1.70A21-105[»]
    4J3EX-ray1.91A21-105[»]
    4J74X-ray1.20A21-105[»]
    4J7DX-ray1.25A21-105[»]
    4J7EX-ray1.63A21-105[»]
    4JRGX-ray1.90A/B21-105[»]
    4JSCX-ray2.50A/B21-105[»]
    ProteinModelPortaliP56273.
    SMRiP56273. Positions 21-108, 281-325, 410-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56273.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 10381SWIBAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni230 – 32293Region IIAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi173 – 1797Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi216 – 29277Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the MDM2/MDM4 family.Curated
    Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 1 SWIB domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri290 – 31930RanBP2-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri420 – 46142RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG013472.

    Family and domain databases

    Gene3Di1.10.245.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR028340. Mdm2.
    IPR015459. MDM2_E3_ligase.
    IPR016495. p53_neg-reg_MDM_2/4.
    IPR003121. SWIB_MDM2_domain.
    IPR001876. Znf_RanBP2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PANTHERiPTHR10360:SF11. PTHR10360:SF11. 1 hit.
    PfamiPF02201. SWIB. 1 hit.
    PF00641. zf-RanBP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500700. MDM2. 1 hit.
    PIRSF006748. p53_MDM_2/4. 1 hit.
    SMARTiSM00184. RING. 1 hit.
    SM00547. ZnF_RBZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF47592. SSF47592. 2 hits.
    PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56273-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLTSTTNCL ENNHISTSDQ EKLVQPTPLL LSLLKSAGAQ KETFTMKEVI    50
    YHLGQYIMAK QLYDEKQQHI VHCSNDPLGE LFGVQEFSVK EPRRLYAMIS 100
    RNLVSANVKE SSEDIFGNVC CFPDKQSSQK EKLQELPDKL IAPASDSKPC 150
    NLSQRKSSNE TEEISSVDHP AEQQRKRHKS DSFSLTFDES LSWWVISGLR 200
    CDRNSSESTD SSSNSDPERH STNDNSEHDS DQFSVEFEVE SVCSDDYSPS 250
    GDEHGVSEEE EINDEVYQVT IYETEESETD SFDVDTEISE ADYWKCPECG 300
    EVNPPLPSYC PRCWTVRKDW LPEQRRKEPP PSKRKLLEIE EDEGFDVPDC 350
    KKSKLTSSQD TNVDKKEAEN IQNSESQETE DCSQPSTSGS IASCSQEVTK 400
    EDSSKESMES SLPLTSIDPC VICQTRPKNG CIVHGRTGHL MACYTCAKKL 450
    KKRNKPCPVC REPIQMIVLT YFS 473
    Length:473
    Mass (Da):53,464
    Last modified:May 30, 2000 - v2
    Checksum:i7DA668DE8B3BEE01
    GO

    Sequence databases

    UniGeneiXl.80570.

    Cross-referencesi

    Sequence databases

    UniGenei Xl.80570.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TTV NMR - A 13-119 [» ]
    1YCQ X-ray 2.30 A 13-119 [» ]
    4IPF X-ray 1.70 A 21-105 [» ]
    4J3E X-ray 1.91 A 21-105 [» ]
    4J74 X-ray 1.20 A 21-105 [» ]
    4J7D X-ray 1.25 A 21-105 [» ]
    4J7E X-ray 1.63 A 21-105 [» ]
    4JRG X-ray 1.90 A/B 21-105 [» ]
    4JSC X-ray 2.50 A/B 21-105 [» ]
    ProteinModelPortali P56273.
    SMRi P56273. Positions 21-108, 281-325, 410-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P56273. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Xenbasei XB-GENE-6255114. mdm2.

    Phylogenomic databases

    HOVERGENi HBG013472.

    Miscellaneous databases

    EvolutionaryTracei P56273.

    Family and domain databases

    Gene3Di 1.10.245.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR028340. Mdm2.
    IPR015459. MDM2_E3_ligase.
    IPR016495. p53_neg-reg_MDM_2/4.
    IPR003121. SWIB_MDM2_domain.
    IPR001876. Znf_RanBP2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    PANTHERi PTHR10360:SF11. PTHR10360:SF11. 1 hit.
    Pfami PF02201. SWIB. 1 hit.
    PF00641. zf-RanBP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500700. MDM2. 1 hit.
    PIRSF006748. p53_MDM_2/4. 1 hit.
    SMARTi SM00184. RING. 1 hit.
    SM00547. ZnF_RBZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47592. SSF47592. 2 hits.
    PROSITEi PS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conservation of structural domains and biochemical activities of the MDM2 protein from Xenopus laevis."
      Marechal V., Elenbaas B., Taneyhill L., Piette J., Mechali M., Nicolas J.-C., Levine A.J., Moreau J.
      Oncogene 14:1427-1433(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain."
      Kussie P.H., Gorina S., Marechal V., Elenbaas B., Moreau J., Levine A.J., Pavletich N.P.
      Science 274:948-953(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 13-119 IN COMPLEX WITH P53.

    Entry informationi

    Entry nameiMDM2_XENLA
    AccessioniPrimary (citable) accession number: P56273
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3