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Protein

E3 ubiquitin-protein ligase Mdm2

Gene

mdm2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri290 – 319RanBP2-typePROSITE-ProRule annotationAdd BLAST30
Zinc fingeri420 – 461RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Mdm2 (EC:6.3.2.-)
Alternative name(s):
Double minute 2 protein
Short name:
Xdm2
p53-binding protein Mdm2
Gene namesi
Name:mdm2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001573341 – 473E3 ubiquitin-protein ligase Mdm2Add BLAST473

Expressioni

Developmental stagei

Expression increases from oocyte stage I/II to reach its maximum in oocyte stage v/vi in unfertilized eggs, and then progressively decreases to become undetectable at the gastrula stage.

Interactioni

Protein-protein interaction databases

IntActiP56273. 1 interactor.

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 26Combined sources3
Helixi28 – 36Combined sources9
Helixi46 – 59Combined sources14
Beta strandi65 – 67Combined sources3
Beta strandi70 – 72Combined sources3
Helixi77 – 82Combined sources6
Beta strandi85 – 88Combined sources4
Helixi92 – 100Combined sources9
Beta strandi103 – 106Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TTVNMR-A13-119[»]
1YCQX-ray2.30A13-119[»]
4IPFX-ray1.70A21-105[»]
4J3EX-ray1.91A21-105[»]
4J74X-ray1.20A21-105[»]
4J7DX-ray1.25A21-105[»]
4J7EX-ray1.63A21-105[»]
4JRGX-ray1.90A/B21-105[»]
4JSCX-ray2.50A/B21-105[»]
4LWTX-ray1.60A21-105[»]
4LWUX-ray1.14A21-105[»]
4LWVX-ray2.32A/B/C21-105[»]
ProteinModelPortaliP56273.
SMRiP56273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 103SWIBAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 322Region IIAdd BLAST93

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi173 – 179Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi216 – 292Asp/Glu-rich (acidic)Add BLAST77

Sequence similaritiesi

Belongs to the MDM2/MDM4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 SWIB domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri290 – 319RanBP2-typePROSITE-ProRule annotationAdd BLAST30
Zinc fingeri420 – 461RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG013472.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13844:SF15. PTHR13844:SF15. 1 hit.
PfamiPF02201. SWIB. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
PIRSFiPIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 2 hits.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTSTTNCL ENNHISTSDQ EKLVQPTPLL LSLLKSAGAQ KETFTMKEVI
60 70 80 90 100
YHLGQYIMAK QLYDEKQQHI VHCSNDPLGE LFGVQEFSVK EPRRLYAMIS
110 120 130 140 150
RNLVSANVKE SSEDIFGNVC CFPDKQSSQK EKLQELPDKL IAPASDSKPC
160 170 180 190 200
NLSQRKSSNE TEEISSVDHP AEQQRKRHKS DSFSLTFDES LSWWVISGLR
210 220 230 240 250
CDRNSSESTD SSSNSDPERH STNDNSEHDS DQFSVEFEVE SVCSDDYSPS
260 270 280 290 300
GDEHGVSEEE EINDEVYQVT IYETEESETD SFDVDTEISE ADYWKCPECG
310 320 330 340 350
EVNPPLPSYC PRCWTVRKDW LPEQRRKEPP PSKRKLLEIE EDEGFDVPDC
360 370 380 390 400
KKSKLTSSQD TNVDKKEAEN IQNSESQETE DCSQPSTSGS IASCSQEVTK
410 420 430 440 450
EDSSKESMES SLPLTSIDPC VICQTRPKNG CIVHGRTGHL MACYTCAKKL
460 470
KKRNKPCPVC REPIQMIVLT YFS
Length:473
Mass (Da):53,464
Last modified:May 30, 2000 - v2
Checksum:i7DA668DE8B3BEE01
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TTVNMR-A13-119[»]
1YCQX-ray2.30A13-119[»]
4IPFX-ray1.70A21-105[»]
4J3EX-ray1.91A21-105[»]
4J74X-ray1.20A21-105[»]
4J7DX-ray1.25A21-105[»]
4J7EX-ray1.63A21-105[»]
4JRGX-ray1.90A/B21-105[»]
4JSCX-ray2.50A/B21-105[»]
4LWTX-ray1.60A21-105[»]
4LWUX-ray1.14A21-105[»]
4LWVX-ray2.32A/B/C21-105[»]
ProteinModelPortaliP56273.
SMRiP56273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56273. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013472.

Miscellaneous databases

EvolutionaryTraceiP56273.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13844:SF15. PTHR13844:SF15. 1 hit.
PfamiPF02201. SWIB. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
PIRSFiPIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 2 hits.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDM2_XENLA
AccessioniPrimary (citable) accession number: P56273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.