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P56273

- MDM2_XENLA

UniProt

P56273 - MDM2_XENLA

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Protein

E3 ubiquitin-protein ligase Mdm2

Gene

mdm2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degration by the proteasome.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri290 – 31930RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri420 – 46142RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. negative regulation of apoptotic process Source: InterPro
  2. negative regulation of cell cycle arrest Source: InterPro
  3. negative regulation of transcription from RNA polymerase II promoter Source: InterPro
  4. protein ubiquitination Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Mdm2 (EC:6.3.2.-)
Alternative name(s):
Double minute 2 protein
Short name:
Xdm2
p53-binding protein Mdm2
Gene namesi
Name:mdm2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6255114. mdm2.

Subcellular locationi

Nucleusnucleoplasm By similarity. Cytoplasm By similarity. Nucleusnucleolus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473E3 ubiquitin-protein ligase Mdm2PRO_0000157334Add
BLAST

Expressioni

Developmental stagei

Expression increases from oocyte stage I/II to reach its maximum in oocyte stage v/vi in unfertilized eggs, and then progressively decreases to become undetectable at the gastrula stage.

Interactioni

Protein-protein interaction databases

IntActiP56273. 1 interaction.

Structurei

Secondary structure

1
473
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 263Combined sources
Helixi28 – 369Combined sources
Helixi46 – 5914Combined sources
Beta strandi65 – 673Combined sources
Beta strandi70 – 723Combined sources
Helixi77 – 826Combined sources
Beta strandi85 – 884Combined sources
Helixi92 – 1009Combined sources
Beta strandi103 – 1064Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TTVNMR-A13-119[»]
1YCQX-ray2.30A13-119[»]
4IPFX-ray1.70A21-105[»]
4J3EX-ray1.91A21-105[»]
4J74X-ray1.20A21-105[»]
4J7DX-ray1.25A21-105[»]
4J7EX-ray1.63A21-105[»]
4JRGX-ray1.90A/B21-105[»]
4JSCX-ray2.50A/B21-105[»]
4LWTX-ray1.60A21-105[»]
4LWUX-ray1.14A21-105[»]
4LWVX-ray2.32A/B/C21-105[»]
ProteinModelPortaliP56273.
SMRiP56273. Positions 21-108, 281-325, 410-472.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 10381SWIBAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 32293Region IIAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi173 – 1797Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi216 – 29277Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the MDM2/MDM4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 SWIB domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri290 – 31930RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri420 – 46142RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG013472.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10360:SF11. PTHR10360:SF11. 1 hit.
PfamiPF02201. SWIB. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
PIRSFiPIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SMARTiSM00184. RING. 1 hit.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF47592. SSF47592. 2 hits.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56273-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLTSTTNCL ENNHISTSDQ EKLVQPTPLL LSLLKSAGAQ KETFTMKEVI
60 70 80 90 100
YHLGQYIMAK QLYDEKQQHI VHCSNDPLGE LFGVQEFSVK EPRRLYAMIS
110 120 130 140 150
RNLVSANVKE SSEDIFGNVC CFPDKQSSQK EKLQELPDKL IAPASDSKPC
160 170 180 190 200
NLSQRKSSNE TEEISSVDHP AEQQRKRHKS DSFSLTFDES LSWWVISGLR
210 220 230 240 250
CDRNSSESTD SSSNSDPERH STNDNSEHDS DQFSVEFEVE SVCSDDYSPS
260 270 280 290 300
GDEHGVSEEE EINDEVYQVT IYETEESETD SFDVDTEISE ADYWKCPECG
310 320 330 340 350
EVNPPLPSYC PRCWTVRKDW LPEQRRKEPP PSKRKLLEIE EDEGFDVPDC
360 370 380 390 400
KKSKLTSSQD TNVDKKEAEN IQNSESQETE DCSQPSTSGS IASCSQEVTK
410 420 430 440 450
EDSSKESMES SLPLTSIDPC VICQTRPKNG CIVHGRTGHL MACYTCAKKL
460 470
KKRNKPCPVC REPIQMIVLT YFS
Length:473
Mass (Da):53,464
Last modified:May 30, 2000 - v2
Checksum:i7DA668DE8B3BEE01
GO

Sequence databases

UniGeneiXl.80570.

Cross-referencesi

Sequence databases

UniGenei Xl.80570.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TTV NMR - A 13-119 [» ]
1YCQ X-ray 2.30 A 13-119 [» ]
4IPF X-ray 1.70 A 21-105 [» ]
4J3E X-ray 1.91 A 21-105 [» ]
4J74 X-ray 1.20 A 21-105 [» ]
4J7D X-ray 1.25 A 21-105 [» ]
4J7E X-ray 1.63 A 21-105 [» ]
4JRG X-ray 1.90 A/B 21-105 [» ]
4JSC X-ray 2.50 A/B 21-105 [» ]
4LWT X-ray 1.60 A 21-105 [» ]
4LWU X-ray 1.14 A 21-105 [» ]
4LWV X-ray 2.32 A/B/C 21-105 [» ]
ProteinModelPortali P56273.
SMRi P56273. Positions 21-108, 281-325, 410-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P56273. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Xenbasei XB-GENE-6255114. mdm2.

Phylogenomic databases

HOVERGENi HBG013472.

Miscellaneous databases

EvolutionaryTracei P56273.

Family and domain databases

Gene3Di 1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProi IPR028340. Mdm2.
IPR015459. MDM2_E3_ligase.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
PANTHERi PTHR10360:SF11. PTHR10360:SF11. 1 hit.
Pfami PF02201. SWIB. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view ]
PIRSFi PIRSF500700. MDM2. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SMARTi SM00184. RING. 1 hit.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view ]
SUPFAMi SSF47592. SSF47592. 2 hits.
PROSITEi PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Conservation of structural domains and biochemical activities of the MDM2 protein from Xenopus laevis."
    Marechal V., Elenbaas B., Taneyhill L., Piette J., Mechali M., Nicolas J.-C., Levine A.J., Moreau J.
    Oncogene 14:1427-1433(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain."
    Kussie P.H., Gorina S., Marechal V., Elenbaas B., Moreau J., Levine A.J., Pavletich N.P.
    Science 274:948-953(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 13-119 IN COMPLEX WITH P53.

Entry informationi

Entry nameiMDM2_XENLA
AccessioniPrimary (citable) accession number: P56273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 26, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3