P56262 (DLHH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative carboxymethylenebutenolidase
EC=3.1.1.45

Alternative name(s):
Dienelactone hydrolase
Short name=DLH

Gene names

Name:	ysgA
Ordered Locus Names:	b3830, JW5853

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Putative carboxymethylenebutenolidase		PRO_0000161578
Sites
	Active site	147	1	By similarity
	Active site	204	1	By similarity
	Active site	236	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56262 [UniParc]. Last modified October 11, 2004. Version 3. Checksum: CD05EAD376CD794F	FASTA	271	29,425
	10 20 30 40 50 60 MATTQQSGFA PAASPLASTI VQTPDDAIVA GFTSIPSQGD NMPAYHARPK QSDGPLPVVI 70 80 90 100 110 120 VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND FADIPTLLSG LVAKVPDSQV 130 140 150 160 170 180 LADLDHVASW ASRNGGDVHR LMITGFCWGG RITWLYAAHN PQLKAAVAWY GKLTGDKSLN 190 200 210 220 230 240 SPKQPVDIAT DLNAPILGLY GGQDNSIPQE SVETMRQALR AANAKAEIIV YPDAGHAFNA 250 260 270 DYRPSYHAAS AEDGWQRMLE WFKQYGGKKS L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAT48226.1. AP009048 Genomic DNA. Translation: BAE77471.1.
RefSeq	YP_026268.1. NC_000913.2. YP_491612.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P56262.
SMR	P56262. Positions 26-265.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12929N.
STRING	511145.b3830.
Proteomic databases
PaxDb	P56262.
PRIDE	P56262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT48226; AAT48226; b3830. BAE77471; BAE77471; BAE77471.
GeneID	12930484. 948320.
KEGG	ecj:Y75_p3348. eco:b3830.
PATRIC	32123161. VBIEscCol129921_3946.
Organism-specific databases
EchoBASE	EB4067.
EcoGene	EG14321. ysgA.
Phylogenomic databases
eggNOG	COG0412.
HOGENOM	HOG000157123.
KO	K01061.
OMA	AGHAFHA.
ProtClustDB	CLSK880795.
Enzyme and pathway databases
BioCyc	EcoCyc:G7804-MONOMER. ECOL316407:JW5853-MONOMER.
Gene expression databases
Genevestigator	P56262.
Family and domain databases
InterPro	IPR002925. Dienelactn_hydro. [Graphical view]
Pfam	PF01738. DLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DLHH_ECOLI

Accession

Primary (citable) accession number: P56262
Secondary accession number(s): Q2M8D5, Q6BEY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program