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Protein

Putative carboxymethylenebutenolidase

Gene

ysgA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471By similarity
Active sitei204 – 2041By similarity
Active sitei236 – 2361By similarity

GO - Molecular functioni

  1. carboxymethylenebutenolidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G7804-MONOMER.
ECOL316407:JW5853-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative carboxymethylenebutenolidase (EC:3.1.1.45)
Alternative name(s):
Dienelactone hydrolase
Short name:
DLH
Gene namesi
Name:ysgA
Ordered Locus Names:b3830, JW5853
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14321. ysgA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Putative carboxymethylenebutenolidasePRO_0000161578Add
BLAST

Proteomic databases

PaxDbiP56262.
PRIDEiP56262.

Expressioni

Gene expression databases

GenevestigatoriP56262.

Interactioni

Protein-protein interaction databases

DIPiDIP-12929N.
STRINGi511145.b3830.

Structurei

3D structure databases

ProteinModelPortaliP56262.
SMRiP56262. Positions 26-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dienelactone hydrolase family.Curated

Phylogenomic databases

eggNOGiCOG0412.
HOGENOMiHOG000157123.
InParanoidiP56262.
KOiK01061.
OMAiCWGGRIS.
OrthoDBiEOG64FKC2.
PhylomeDBiP56262.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002925. Dienelactn_hydro.
[Graphical view]
PfamiPF01738. DLH. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P56262-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATTQQSGFA PAASPLASTI VQTPDDAIVA GFTSIPSQGD NMPAYHARPK
60 70 80 90 100
QSDGPLPVVI VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND
110 120 130 140 150
FADIPTLLSG LVAKVPDSQV LADLDHVASW ASRNGGDVHR LMITGFCWGG
160 170 180 190 200
RITWLYAAHN PQLKAAVAWY GKLTGDKSLN SPKQPVDIAT DLNAPILGLY
210 220 230 240 250
GGQDNSIPQE SVETMRQALR AANAKAEIIV YPDAGHAFNA DYRPSYHAAS
260 270
AEDGWQRMLE WFKQYGGKKS L
Length:271
Mass (Da):29,425
Last modified:October 11, 2004 - v3
Checksum:iCD05EAD376CD794F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAT48226.1.
AP009048 Genomic DNA. Translation: BAE77471.1.
RefSeqiYP_026268.1. NC_000913.3.
YP_491612.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAT48226; AAT48226; b3830.
BAE77471; BAE77471; BAE77471.
GeneIDi12930484.
948320.
KEGGiecj:Y75_p3348.
eco:b3830.
PATRICi32123161. VBIEscCol129921_3946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAT48226.1.
AP009048 Genomic DNA. Translation: BAE77471.1.
RefSeqiYP_026268.1. NC_000913.3.
YP_491612.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP56262.
SMRiP56262. Positions 26-265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-12929N.
STRINGi511145.b3830.

Proteomic databases

PaxDbiP56262.
PRIDEiP56262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48226; AAT48226; b3830.
BAE77471; BAE77471; BAE77471.
GeneIDi12930484.
948320.
KEGGiecj:Y75_p3348.
eco:b3830.
PATRICi32123161. VBIEscCol129921_3946.

Organism-specific databases

EchoBASEiEB4067.
EcoGeneiEG14321. ysgA.

Phylogenomic databases

eggNOGiCOG0412.
HOGENOMiHOG000157123.
InParanoidiP56262.
KOiK01061.
OMAiCWGGRIS.
OrthoDBiEOG64FKC2.
PhylomeDBiP56262.

Enzyme and pathway databases

BioCyciEcoCyc:G7804-MONOMER.
ECOL316407:JW5853-MONOMER.

Miscellaneous databases

PROiP56262.

Gene expression databases

GenevestigatoriP56262.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002925. Dienelactn_hydro.
[Graphical view]
PfamiPF01738. DLH. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: SEQUENCE REVISION.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiDLHH_ECOLI
AccessioniPrimary (citable) accession number: P56262
Secondary accession number(s): Q2M8D5, Q6BEY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 11, 2004
Last modified: January 7, 2015
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.