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Protein

TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase

Gene

wecF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis.1 Publication

Catalytic activityi

dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose + N-acetyl-beta-D-mannosaminouronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = dTDP + 4-acetamido-4,6-dideoxy-alpha-D-galactosyl-(1->4)-N-acetyl-beta-D-mannosaminouronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol.1 Publication

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • enterobacterial common antigen biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G7800-MONOMER.
ECOL316407:JW5596-MONOMER.
MetaCyc:G7800-MONOMER.
UniPathwayiUPA00566.

Protein family/group databases

CAZyiGT56. Glycosyltransferase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase (EC:2.4.1.325)
Alternative name(s):
4-alpha-L-fucosyltransferase
TDP-Fuc4NAc:lipid II Fuc4NAc transferase
Short name:
Fuc4NAc transferase
Gene namesi
Name:wecF
Synonyms:rffT, yifM
Ordered Locus Names:b4481, JW5596
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14320. rffT.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferasePRO_0000216180Add
BLAST

Proteomic databases

PaxDbiP56258.
PRIDEiP56258.

Interactioni

Protein-protein interaction databases

BioGridi4262106. 182 interactions.
IntActiP56258. 3 interactions.
STRINGi511145.b4481.

Structurei

3D structure databases

ProteinModelPortaliP56258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 56 family.Curated

Phylogenomic databases

eggNOGiENOG4105F18. Bacteria.
ENOG410XT9P. LUCA.
HOGENOMiHOG000249654.
KOiK12582.
OMAiVIVPMGY.

Family and domain databases

HAMAPiMF_01002. WecF_RffT. 1 hit.
InterProiIPR009993. WecF.
[Graphical view]
PfamiPF07429. Glyco_transf_56. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLIHVLGS DIPHHNRTVL RFFNDALAAT SEHAREFMVV GKDDGLSDSC
60 70 80 90 100
PALSVQFFPG KKSLAEAVIA KAKANRQQRF FFHGQFNPTL WLALLSGGIK
110 120 130 140 150
PSQFFWHIWG ADLYELSSGL RYKLFYPLRR LAQKRVGCVF ATRGDLSFFA
160 170 180 190 200
KTHPKVRGEL LFFPTRMDPS LNTMANDRQR EGKMTILVGN SGDRSNEHIA
210 220 230 240 250
ALRAVHQQFG DTVKVVVPMG YPPNNEAYIE EVRQAGLELF SEENLQILSE
260 270 280 290 300
KLEFDAYLAL LRQCDLGYFI FARQQGIGTL CLLIQAGIPC VLNRENPFWQ
310 320 330 340 350
DMTEQHLPVL FTTDDLNEDI VREAQRQLAS VDKNTIAFFS PNYLQGWQRA

LAIAAREVA
Length:359
Mass (Da):40,640
Last modified:March 5, 2002 - v2
Checksum:i092E2813918DC1D1
GO

Sequence cautioni

The sequence AAA67593 differs from that shown. Reason: Frameshift at positions 60, 67, 327 and 354. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321A → G in AAA67593 (PubMed:1379743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375882 Genomic DNA. Translation: AAK62972.1.
M87049 Genomic DNA. Translation: AAA67593.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48215.1.
AP009048 Genomic DNA. Translation: BAE77505.1.
RefSeqiWP_000217234.1. NZ_LN832404.1.
YP_026257.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48215; AAT48215; b4481.
BAE77505; BAE77505; BAE77505.
GeneIDi2847677.
KEGGiecj:JW5596.
eco:b4481.
PATRICi32123079. VBIEscCol129921_3909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375882 Genomic DNA. Translation: AAK62972.1.
M87049 Genomic DNA. Translation: AAA67593.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48215.1.
AP009048 Genomic DNA. Translation: BAE77505.1.
RefSeqiWP_000217234.1. NZ_LN832404.1.
YP_026257.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP56258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262106. 182 interactions.
IntActiP56258. 3 interactions.
STRINGi511145.b4481.

Protein family/group databases

CAZyiGT56. Glycosyltransferase Family 56.

Proteomic databases

PaxDbiP56258.
PRIDEiP56258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48215; AAT48215; b4481.
BAE77505; BAE77505; BAE77505.
GeneIDi2847677.
KEGGiecj:JW5596.
eco:b4481.
PATRICi32123079. VBIEscCol129921_3909.

Organism-specific databases

EchoBASEiEB4066.
EcoGeneiEG14320. rffT.

Phylogenomic databases

eggNOGiENOG4105F18. Bacteria.
ENOG410XT9P. LUCA.
HOGENOMiHOG000249654.
KOiK12582.
OMAiVIVPMGY.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:G7800-MONOMER.
ECOL316407:JW5596-MONOMER.
MetaCyc:G7800-MONOMER.

Miscellaneous databases

PROiP56258.

Family and domain databases

HAMAPiMF_01002. WecF_RffT. 1 hit.
InterProiIPR009993. WecF.
[Graphical view]
PfamiPF07429. Glyco_transf_56. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWECF_ECOLI
AccessioniPrimary (citable) accession number: P56258
Secondary accession number(s): Q2M8A1, Q93DX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 5, 2002
Last modified: September 7, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.