Reviewed, UniProtKB/Swiss-Prot P56258 (WECF_ECOLI)

Names and origin

Protein names

Recommended name:
    4-alpha-L-fucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    TDP-Fuc4NAc:lipid II Fuc4NAc transferase
      Short name=Fuc4NAc transferase

Gene names

Name:	wecF
Synonyms:	rffT, yifM
Ordered Locus Names:	b4481, JW5596

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis. HAMAP MF_01002
Catalytic activity	TDP-Fuc4NAc + Und-PP-GlcNAc-ManNAcA = TDP + Und-PP-GlcNAc-ManNAcA-Fuc4NAc. HAMAP MF_01002
Pathway	Bacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis. HAMAP MF_01002
Subcellular location	Cell inner membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 56 family.
Sequence caution	The sequence AAA67593.1 differs from that shown. Reason: Frameshift at positions 60, 67, 327 and 354.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	enterobacterial common antigen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	4-alpha-L-fucosyltransferase HAMAP MF_01002		PRO_0000216180
Experimental info
	Sequence conflict	132	1	A → G in AAA67593. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P56258-1 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: 092E2813918DC1D1	FASTA	359	40,640
	10 20 30 40 50 60 MTVLIHVLGS DIPHHNRTVL RFFNDALAAT SEHAREFMVV GKDDGLSDSC PALSVQFFPG 70 80 90 100 110 120 KKSLAEAVIA KAKANRQQRF FFHGQFNPTL WLALLSGGIK PSQFFWHIWG ADLYELSSGL 130 140 150 160 170 180 RYKLFYPLRR LAQKRVGCVF ATRGDLSFFA KTHPKVRGEL LFFPTRMDPS LNTMANDRQR 190 200 210 220 230 240 EGKMTILVGN SGDRSNEHIA ALRAVHQQFG DTVKVVVPMG YPPNNEAYIE EVRQAGLELF 250 260 270 280 290 300 SEENLQILSE KLEFDAYLAL LRQCDLGYFI FARQQGIGTL CLLIQAGIPC VLNRENPFWQ 310 320 330 340 350 DMTEQHLPVL FTTDDLNEDI VREAQRQLAS VDKNTIAFFS PNYLQGWQRA LAIAAREVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the structural gene for the TDP-Fuc4NAc:Lipid II Fuc4NAc transferase involved in synthesis of enterobacterial common antigen in Escherichia coli K-12." Rahman A., Barr K., Rick P.D. J. Bacteriol. 183:6509-6516(2001) [PubMed: 11673418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed: 1379743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF375882 Genomic DNA. Translation: AAK62972.1. M87049 Genomic DNA. Translation: AAA67593.1. Frameshift. U00096 Genomic DNA. Translation: AAT48215.1. AP009048 Genomic DNA. Translation: BAE77505.1.
RefSeq	AP_004004.1. YP_026257.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT56. Glycosyltransferase Family 56.
Genome annotation databases
GeneID	2847677.
GenomeReviews	Gene locus JW5596 in contig AP009048_GR. Gene locus b4481 in contig U00096_GR.
KEGG	ecj:JW5596. eco:b4481.
Organism-specific databases
EchoBASE	EB4066.
EcoGene	EG14320. rffT.
CMR	Search...
Phylogenomic databases
HOGENOM	P56258.
OMA	P56258. IPHHNQT.
Enzyme and pathway databases
BioCyc	EcoCyc:G7800-MON. MetaCyc:G7800-MON.
Family and domain databases
HAMAP	MF_01002. [Tree]
InterPro	IPR009993. 4-alpha-FUT. [Graphical view]
Pfam	PF07429. Fuc4NAc_transf. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WECF_ECOLI

Accession

Primary (citable) accession number: P56258
Secondary accession number(s): Q2M8A1, Q93DX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	March 5, 2002
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)