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P56255

- PCRA_GEOSE

UniProt

P56255 - PCRA_GEOSE

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Protein
ATP-dependent DNA helicase PcrA
Gene
pcrA
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei287 – 2871ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166ATP
Nucleotide bindingi34 – 396ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: InterPro
  3. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA unwinding involved in DNA replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PcrA (EC:3.6.4.12)
Gene namesi
Name:pcrA
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724ATP-dependent DNA helicase PcrA
PRO_0000102050Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97
Helixi14 – 218
Beta strandi27 – 315
Helixi37 – 5014
Helixi56 – 583
Beta strandi59 – 657
Helixi66 – 8015
Helixi81 – 844
Beta strandi87 – 915
Helixi92 – 10312
Helixi104 – 1074
Beta strandi114 – 1163
Helixi118 – 13013
Turni135 – 1384
Helixi141 – 15313
Helixi158 – 1614
Helixi169 – 18618
Beta strandi189 – 1913
Helixi194 – 20512
Helixi207 – 21610
Beta strandi218 – 2247
Helixi225 – 2273
Helixi230 – 24011
Turni241 – 2433
Beta strandi246 – 2505
Helixi252 – 2543
Helixi258 – 2603
Helixi265 – 2684
Helixi270 – 2734
Beta strandi278 – 2825
Beta strandi286 – 2883
Helixi290 – 30011
Beta strandi304 – 3063
Beta strandi312 – 3154
Beta strandi321 – 3288
Helixi329 – 34416
Turni345 – 3473
Helixi351 – 3533
Beta strandi354 – 3607
Helixi361 – 3633
Helixi364 – 37310
Beta strandi378 – 3814
Helixi386 – 3883
Helixi390 – 40314
Helixi408 – 4147
Beta strandi415 – 4184
Turni421 – 4233
Helixi424 – 43512
Turni436 – 4383
Turni442 – 4465
Turni449 – 4513
Turni454 – 4618
Helixi462 – 47413
Turni475 – 4773
Helixi480 – 49011
Helixi493 – 5008
Helixi503 – 52523
Helixi531 – 5377
Turni538 – 5403
Turni544 – 5463
Beta strandi559 – 5635
Helixi564 – 5674
Beta strandi572 – 5776
Turni584 – 5863
Helixi588 – 5914
Helixi594 – 60815
Beta strandi611 – 62515
Beta strandi628 – 6303
Helixi636 – 6405
Helixi643 – 6453
Beta strandi646 – 6483

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PJRX-ray2.50A1-724[»]
1QHGX-ray2.50A1-724[»]
1QHHX-ray2.50A1-167[»]
B168-440[»]
C441-555[»]
D556-724[»]
2PJRX-ray2.90A/F1-548[»]
B/G556-650[»]
3PJRX-ray3.30A1-724[»]
ProteinModelPortaliP56255.
SMRiP56255. Positions 4-652.

Miscellaneous databases

EvolutionaryTraceiP56255.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 289280UvrD-like helicase ATP-binding
Add
BLAST
Domaini290 – 569280UvrD-like helicase C-terminal
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01073. pcrA. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56255-1 [UniParc]FASTAAdd to Basket

« Hide

MNFLSEQLLA HLNKEQQEAV RTTEGPLLIM AGAGSGKTRV LTHRIAYLMA    50
EKHVAPWNIL AITFTNKAAR EMRERVQSLL GGAAEDVWIS TFHSMCVRIL 100
RRDIDRIGIN RNFSILDPTD QLSVMKTILK EKNIDPKKFE PRTILGTISA 150
AKNELLPPEQ FAKRASTYYE KVVSDVYQEY QQRLLRNHSL DFDDLIMTTI 200
QLFDRVPDVL HYYQYKFQYI HIDEYQDTNR AQYTLVKKLA ERFQNICAVG 250
DADQSIYRWR GADIQNILSF ERDYPNAKVI LLEQNYRSTK RILQAANEVI 300
EHNVNRKPKR IWTENPEGKP ILYYEAMNEA DEAQFVAGRI REAVERGERR 350
YRDFAVLYRT NAQSRVMEEM LLKANIPYQI VGGLKFYDRK EIKDILAYLR 400
VIANPDDDLS LLRIINVPKR GIGASTIDKL VRYAADHELS LFEALGELEM 450
IGLGAKAAGA LAAFRSQLEQ WTQLQEYVSV TELVEEVLDK SGYREMLKAE 500
RTIEAQSRLE NLDEFLSVTK HFENVSDDKS LIAFLTDLAL ISDLDELDGT 550
EQAAEGDAVM LMTLHAAKGL EFPVVFLIGM EEGIFPHNRS LEDDDEMEEE 600
RRLAYVGITR AEEELVLTSA QMRTLFGNIQ MDPPSRFLNE IPAHLLETAS 650
RRQAGASRPA VSRPQASGAV GSWKVGDRAN HRKWGIGTVV SVRGGGDDQE 700
LDIAFPSPIG IKRLLAKFAP IEKV 724
Length:724
Mass (Da):82,488
Last modified:July 15, 1998 - v1
Checksum:i6D20CA468BFB4291
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PJR X-ray 2.50 A 1-724 [» ]
1QHG X-ray 2.50 A 1-724 [» ]
1QHH X-ray 2.50 A 1-167 [» ]
B 168-440 [» ]
C 441-555 [» ]
D 556-724 [» ]
2PJR X-ray 2.90 A/F 1-548 [» ]
B/G 556-650 [» ]
3PJR X-ray 3.30 A 1-724 [» ]
ProteinModelPortali P56255.
SMRi P56255. Positions 4-652.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P56255.

Family and domain databases

Gene3Di 1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProi IPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view ]
PANTHERi PTHR11070. PTHR11070. 1 hit.
Pfami PF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01073. pcrA. 1 hit.
PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism."
    Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B.
    Nucleic Acids Res. 26:2686-2693(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
  3. "Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism."
    Velankar S.S., Soultanas P., Dillingham M.S., Subramanya H.S., Wigley D.B.
    Cell 97:75-84(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
  4. "DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase."
    Soultanas P., Dillingham M.S., Velankar S.S., Wigley D.B.
    J. Mol. Biol. 290:137-148(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH ATP.
  5. "Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation."
    Dillingham M.S., Soultanas P., Wigley D.B.
    Nucleic Acids Res. 27:3310-3317(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiPCRA_GEOSE
AccessioniPrimary (citable) accession number: P56255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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