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P56255

- PCRA_GEOSE

UniProt

P56255 - PCRA_GEOSE

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Protein

ATP-dependent DNA helicase PcrA

Gene

pcrA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei287 – 2871ATP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166ATP1 PublicationPROSITE-ProRule annotation
Nucleotide bindingi34 – 396ATP1 PublicationPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: InterPro
  3. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA unwinding involved in DNA replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PcrA (EC:3.6.4.12)
Gene namesi
Name:pcrA
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724ATP-dependent DNA helicase PcrAPRO_0000102050Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
724
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97Combined sources
Helixi14 – 218Combined sources
Beta strandi27 – 315Combined sources
Helixi37 – 5014Combined sources
Helixi56 – 583Combined sources
Beta strandi59 – 657Combined sources
Helixi66 – 8015Combined sources
Helixi81 – 844Combined sources
Beta strandi87 – 915Combined sources
Helixi92 – 10312Combined sources
Helixi104 – 1074Combined sources
Beta strandi114 – 1163Combined sources
Helixi118 – 13013Combined sources
Turni135 – 1384Combined sources
Helixi141 – 15313Combined sources
Helixi158 – 1614Combined sources
Helixi169 – 18618Combined sources
Beta strandi189 – 1913Combined sources
Helixi194 – 20512Combined sources
Helixi207 – 21610Combined sources
Beta strandi218 – 2247Combined sources
Helixi225 – 2273Combined sources
Helixi230 – 24011Combined sources
Turni241 – 2433Combined sources
Beta strandi246 – 2505Combined sources
Helixi252 – 2543Combined sources
Helixi258 – 2603Combined sources
Helixi265 – 2684Combined sources
Helixi270 – 2734Combined sources
Beta strandi278 – 2825Combined sources
Beta strandi286 – 2883Combined sources
Helixi290 – 30011Combined sources
Beta strandi304 – 3063Combined sources
Beta strandi312 – 3154Combined sources
Beta strandi321 – 3288Combined sources
Helixi329 – 34416Combined sources
Turni345 – 3473Combined sources
Helixi351 – 3533Combined sources
Beta strandi354 – 3607Combined sources
Helixi361 – 3633Combined sources
Helixi364 – 37310Combined sources
Beta strandi378 – 3814Combined sources
Helixi386 – 3883Combined sources
Helixi390 – 40314Combined sources
Helixi408 – 4147Combined sources
Beta strandi415 – 4184Combined sources
Turni421 – 4233Combined sources
Helixi424 – 43512Combined sources
Turni436 – 4383Combined sources
Turni442 – 4465Combined sources
Turni449 – 4513Combined sources
Turni454 – 4618Combined sources
Helixi462 – 47413Combined sources
Turni475 – 4773Combined sources
Helixi480 – 49011Combined sources
Helixi493 – 5008Combined sources
Helixi503 – 52523Combined sources
Helixi531 – 5377Combined sources
Turni538 – 5403Combined sources
Turni544 – 5463Combined sources
Beta strandi559 – 5635Combined sources
Helixi564 – 5674Combined sources
Beta strandi572 – 5776Combined sources
Turni584 – 5863Combined sources
Helixi588 – 5914Combined sources
Helixi594 – 60815Combined sources
Beta strandi611 – 62515Combined sources
Beta strandi628 – 6303Combined sources
Helixi636 – 6405Combined sources
Helixi643 – 6453Combined sources
Beta strandi646 – 6483Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PJRX-ray2.50A1-724[»]
1QHGX-ray2.50A1-724[»]
1QHHX-ray2.50A1-167[»]
B168-440[»]
C441-555[»]
D556-724[»]
2PJRX-ray2.90A/F1-548[»]
B/G556-650[»]
3PJRX-ray3.30A1-724[»]
ProteinModelPortaliP56255.
SMRiP56255. Positions 4-652.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56255.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 289280UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini290 – 569280UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01073. pcrA. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56255-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNFLSEQLLA HLNKEQQEAV RTTEGPLLIM AGAGSGKTRV LTHRIAYLMA
60 70 80 90 100
EKHVAPWNIL AITFTNKAAR EMRERVQSLL GGAAEDVWIS TFHSMCVRIL
110 120 130 140 150
RRDIDRIGIN RNFSILDPTD QLSVMKTILK EKNIDPKKFE PRTILGTISA
160 170 180 190 200
AKNELLPPEQ FAKRASTYYE KVVSDVYQEY QQRLLRNHSL DFDDLIMTTI
210 220 230 240 250
QLFDRVPDVL HYYQYKFQYI HIDEYQDTNR AQYTLVKKLA ERFQNICAVG
260 270 280 290 300
DADQSIYRWR GADIQNILSF ERDYPNAKVI LLEQNYRSTK RILQAANEVI
310 320 330 340 350
EHNVNRKPKR IWTENPEGKP ILYYEAMNEA DEAQFVAGRI REAVERGERR
360 370 380 390 400
YRDFAVLYRT NAQSRVMEEM LLKANIPYQI VGGLKFYDRK EIKDILAYLR
410 420 430 440 450
VIANPDDDLS LLRIINVPKR GIGASTIDKL VRYAADHELS LFEALGELEM
460 470 480 490 500
IGLGAKAAGA LAAFRSQLEQ WTQLQEYVSV TELVEEVLDK SGYREMLKAE
510 520 530 540 550
RTIEAQSRLE NLDEFLSVTK HFENVSDDKS LIAFLTDLAL ISDLDELDGT
560 570 580 590 600
EQAAEGDAVM LMTLHAAKGL EFPVVFLIGM EEGIFPHNRS LEDDDEMEEE
610 620 630 640 650
RRLAYVGITR AEEELVLTSA QMRTLFGNIQ MDPPSRFLNE IPAHLLETAS
660 670 680 690 700
RRQAGASRPA VSRPQASGAV GSWKVGDRAN HRKWGIGTVV SVRGGGDDQE
710 720
LDIAFPSPIG IKRLLAKFAP IEKV
Length:724
Mass (Da):82,488
Last modified:July 15, 1998 - v1
Checksum:i6D20CA468BFB4291
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PJR X-ray 2.50 A 1-724 [» ]
1QHG X-ray 2.50 A 1-724 [» ]
1QHH X-ray 2.50 A 1-167 [» ]
B 168-440 [» ]
C 441-555 [» ]
D 556-724 [» ]
2PJR X-ray 2.90 A/F 1-548 [» ]
B/G 556-650 [» ]
3PJR X-ray 3.30 A 1-724 [» ]
ProteinModelPortali P56255.
SMRi P56255. Positions 4-652.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P56255.

Family and domain databases

Gene3Di 1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProi IPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view ]
PANTHERi PTHR11070. PTHR11070. 1 hit.
Pfami PF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01073. pcrA. 1 hit.
PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism."
    Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B.
    Nucleic Acids Res. 26:2686-2693(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
  3. "Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism."
    Velankar S.S., Soultanas P., Dillingham M.S., Subramanya H.S., Wigley D.B.
    Cell 97:75-84(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
  4. "DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase."
    Soultanas P., Dillingham M.S., Velankar S.S., Wigley D.B.
    J. Mol. Biol. 290:137-148(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH ATP.
  5. "Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation."
    Dillingham M.S., Soultanas P., Wigley D.B.
    Nucleic Acids Res. 27:3310-3317(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiPCRA_GEOSE
AccessioniPrimary (citable) accession number: P56255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3