Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase PcrA

Gene

pcrA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287ATP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16ATPPROSITE-ProRule annotation1 Publication6
Nucleotide bindingi34 – 39ATPPROSITE-ProRule annotation1 Publication6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.12. 623.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PcrA (EC:3.6.4.12)
Gene namesi
Name:pcrA
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020501 – 724ATP-dependent DNA helicase PcrAAdd BLAST724

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1724
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 9Combined sources7
Helixi14 – 21Combined sources8
Beta strandi27 – 31Combined sources5
Helixi37 – 50Combined sources14
Helixi56 – 58Combined sources3
Beta strandi59 – 65Combined sources7
Helixi66 – 80Combined sources15
Helixi81 – 84Combined sources4
Beta strandi87 – 91Combined sources5
Helixi92 – 103Combined sources12
Helixi104 – 107Combined sources4
Beta strandi114 – 116Combined sources3
Helixi118 – 130Combined sources13
Turni135 – 138Combined sources4
Helixi141 – 153Combined sources13
Helixi158 – 161Combined sources4
Helixi169 – 186Combined sources18
Beta strandi189 – 191Combined sources3
Helixi194 – 205Combined sources12
Helixi207 – 216Combined sources10
Beta strandi218 – 224Combined sources7
Helixi225 – 227Combined sources3
Helixi230 – 240Combined sources11
Turni241 – 243Combined sources3
Beta strandi246 – 250Combined sources5
Helixi252 – 254Combined sources3
Helixi258 – 260Combined sources3
Helixi265 – 268Combined sources4
Helixi270 – 273Combined sources4
Beta strandi278 – 282Combined sources5
Beta strandi286 – 288Combined sources3
Helixi290 – 300Combined sources11
Beta strandi304 – 306Combined sources3
Beta strandi312 – 315Combined sources4
Beta strandi321 – 328Combined sources8
Helixi329 – 344Combined sources16
Turni345 – 347Combined sources3
Helixi351 – 353Combined sources3
Beta strandi354 – 360Combined sources7
Helixi361 – 363Combined sources3
Helixi364 – 373Combined sources10
Beta strandi378 – 381Combined sources4
Helixi386 – 388Combined sources3
Helixi390 – 403Combined sources14
Helixi408 – 414Combined sources7
Beta strandi415 – 418Combined sources4
Turni421 – 423Combined sources3
Helixi424 – 435Combined sources12
Turni436 – 438Combined sources3
Turni442 – 446Combined sources5
Turni449 – 451Combined sources3
Turni454 – 461Combined sources8
Helixi462 – 474Combined sources13
Turni475 – 477Combined sources3
Helixi480 – 490Combined sources11
Helixi493 – 500Combined sources8
Helixi503 – 525Combined sources23
Helixi531 – 537Combined sources7
Turni538 – 540Combined sources3
Turni544 – 546Combined sources3
Beta strandi559 – 563Combined sources5
Helixi564 – 567Combined sources4
Beta strandi572 – 577Combined sources6
Turni584 – 586Combined sources3
Helixi588 – 591Combined sources4
Helixi594 – 608Combined sources15
Beta strandi611 – 625Combined sources15
Beta strandi628 – 630Combined sources3
Helixi636 – 640Combined sources5
Helixi643 – 645Combined sources3
Beta strandi646 – 648Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PJRX-ray2.50A1-724[»]
1QHGX-ray2.50A1-724[»]
1QHHX-ray2.50A1-167[»]
B168-440[»]
C441-555[»]
D556-724[»]
2PJRX-ray2.90A/F1-548[»]
B/G556-650[»]
3PJRX-ray3.30A1-724[»]
5DMAX-ray1.53A673-724[»]
ProteinModelPortaliP56255.
SMRiP56255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56255.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 289UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST280
Domaini290 – 569UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01073. pcrA. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFLSEQLLA HLNKEQQEAV RTTEGPLLIM AGAGSGKTRV LTHRIAYLMA
60 70 80 90 100
EKHVAPWNIL AITFTNKAAR EMRERVQSLL GGAAEDVWIS TFHSMCVRIL
110 120 130 140 150
RRDIDRIGIN RNFSILDPTD QLSVMKTILK EKNIDPKKFE PRTILGTISA
160 170 180 190 200
AKNELLPPEQ FAKRASTYYE KVVSDVYQEY QQRLLRNHSL DFDDLIMTTI
210 220 230 240 250
QLFDRVPDVL HYYQYKFQYI HIDEYQDTNR AQYTLVKKLA ERFQNICAVG
260 270 280 290 300
DADQSIYRWR GADIQNILSF ERDYPNAKVI LLEQNYRSTK RILQAANEVI
310 320 330 340 350
EHNVNRKPKR IWTENPEGKP ILYYEAMNEA DEAQFVAGRI REAVERGERR
360 370 380 390 400
YRDFAVLYRT NAQSRVMEEM LLKANIPYQI VGGLKFYDRK EIKDILAYLR
410 420 430 440 450
VIANPDDDLS LLRIINVPKR GIGASTIDKL VRYAADHELS LFEALGELEM
460 470 480 490 500
IGLGAKAAGA LAAFRSQLEQ WTQLQEYVSV TELVEEVLDK SGYREMLKAE
510 520 530 540 550
RTIEAQSRLE NLDEFLSVTK HFENVSDDKS LIAFLTDLAL ISDLDELDGT
560 570 580 590 600
EQAAEGDAVM LMTLHAAKGL EFPVVFLIGM EEGIFPHNRS LEDDDEMEEE
610 620 630 640 650
RRLAYVGITR AEEELVLTSA QMRTLFGNIQ MDPPSRFLNE IPAHLLETAS
660 670 680 690 700
RRQAGASRPA VSRPQASGAV GSWKVGDRAN HRKWGIGTVV SVRGGGDDQE
710 720
LDIAFPSPIG IKRLLAKFAP IEKV
Length:724
Mass (Da):82,488
Last modified:July 15, 1998 - v1
Checksum:i6D20CA468BFB4291
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PJRX-ray2.50A1-724[»]
1QHGX-ray2.50A1-724[»]
1QHHX-ray2.50A1-167[»]
B168-440[»]
C441-555[»]
D556-724[»]
2PJRX-ray2.90A/F1-548[»]
B/G556-650[»]
3PJRX-ray3.30A1-724[»]
5DMAX-ray1.53A673-724[»]
ProteinModelPortaliP56255.
SMRiP56255.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.6.4.12. 623.

Miscellaneous databases

EvolutionaryTraceiP56255.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01073. pcrA. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCRA_GEOSE
AccessioniPrimary (citable) accession number: P56255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.