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P56255 (PCRA_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent DNA helicase PcrA
EC=3.6.4.12
Gene names
Name:pcrA
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double stranded regions of DNA.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Monomer.

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Contains 1 uvrD-like helicase ATP-binding domain.

Contains 1 uvrD-like helicase C-terminal domain.

Ontologies

Keywords
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA unwinding involved in replication

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent DNA helicase activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 724724ATP-dependent DNA helicase PcrA
PRO_0000102050

Regions

Domain10 – 289280UvrD-like helicase ATP-binding
Domain290 – 569280UvrD-like helicase C-terminal
Nucleotide binding11 – 166ATP
Nucleotide binding34 – 396ATP

Sites

Binding site2871ATP

Secondary structure

1 ........................................................................................................................ 724
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56255 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 6D20CA468BFB4291

FASTA72482,488
        10         20         30         40         50         60 
MNFLSEQLLA HLNKEQQEAV RTTEGPLLIM AGAGSGKTRV LTHRIAYLMA EKHVAPWNIL 

        70         80         90        100        110        120 
AITFTNKAAR EMRERVQSLL GGAAEDVWIS TFHSMCVRIL RRDIDRIGIN RNFSILDPTD 

       130        140        150        160        170        180 
QLSVMKTILK EKNIDPKKFE PRTILGTISA AKNELLPPEQ FAKRASTYYE KVVSDVYQEY 

       190        200        210        220        230        240 
QQRLLRNHSL DFDDLIMTTI QLFDRVPDVL HYYQYKFQYI HIDEYQDTNR AQYTLVKKLA 

       250        260        270        280        290        300 
ERFQNICAVG DADQSIYRWR GADIQNILSF ERDYPNAKVI LLEQNYRSTK RILQAANEVI 

       310        320        330        340        350        360 
EHNVNRKPKR IWTENPEGKP ILYYEAMNEA DEAQFVAGRI REAVERGERR YRDFAVLYRT 

       370        380        390        400        410        420 
NAQSRVMEEM LLKANIPYQI VGGLKFYDRK EIKDILAYLR VIANPDDDLS LLRIINVPKR 

       430        440        450        460        470        480 
GIGASTIDKL VRYAADHELS LFEALGELEM IGLGAKAAGA LAAFRSQLEQ WTQLQEYVSV 

       490        500        510        520        530        540 
TELVEEVLDK SGYREMLKAE RTIEAQSRLE NLDEFLSVTK HFENVSDDKS LIAFLTDLAL 

       550        560        570        580        590        600 
ISDLDELDGT EQAAEGDAVM LMTLHAAKGL EFPVVFLIGM EEGIFPHNRS LEDDDEMEEE 

       610        620        630        640        650        660 
RRLAYVGITR AEEELVLTSA QMRTLFGNIQ MDPPSRFLNE IPAHLLETAS RRQAGASRPA 

       670        680        690        700        710        720 
VSRPQASGAV GSWKVGDRAN HRKWGIGTVV SVRGGGDDQE LDIAFPSPIG IKRLLAKFAP 


IEKV

« Hide

References

[1]"Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism."
Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B.
Nucleic Acids Res. 26:2686-2693(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[2]"Crystal structure of a DExx box DNA helicase."
Subramanya H.S., Bird L.E., Brannigan J.A., Wigley D.B.
Nature 384:379-383(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
[3]"Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism."
Velankar S.S., Soultanas P., Dillingham M.S., Subramanya H.S., Wigley D.B.
Cell 97:75-84(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
[4]"DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase."
Soultanas P., Dillingham M.S., Velankar S.S., Wigley D.B.
J. Mol. Biol. 290:137-148(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH ATP.
[5]"Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation."
Dillingham M.S., Soultanas P., Wigley D.B.
Nucleic Acids Res. 27:3310-3317(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PJRX-ray2.50A1-724[»]
1QHGX-ray2.50A1-724[»]
1QHHX-ray2.50A1-167[»]
B168-440[»]
C441-555[»]
D556-724[»]
2PJRX-ray2.90A/F1-548[»]
B/G556-650[»]
3PJRX-ray3.30A1-724[»]
ProteinModelPortalP56255.
SMRP56255. Positions 4-652.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.160. 1 hit.
InterProIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERPTHR11070. PTHR11070. 1 hit.
PfamPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01073. pcrA. 1 hit.
PROSITEPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP56255.

Entry information

Entry namePCRA_GEOSE
AccessionPrimary (citable) accession number: P56255
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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