P56254KAB1_OLDAFKalata-B1OAK1Oldenlandia affinisEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeRubioideaeSpermacoceaeHedyotis-Oldenlandia complexOldenlandiaBiosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis.NUCLEOTIDE SEQUENCE [MRNA]Elucidation of the primary and three-dimensional structure of the uterotonic polypeptide kalata B1.PROTEIN SEQUENCE OF 89-117MASS SPECTROMETRYSTRUCTURE BY NMR OF 89-109DISULFIDE BONDSThe cyclotide fingerprint in Oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.PROTEIN SEQUENCE OF 89-117FUNCTIONMASS SPECTROMETRYOXIDATIONAn unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides.SYNTHESIS OF 89-117ANTIBACTERIAL ACTIVITYRefined structure and metal binding site of the kalata B1 peptide.STRUCTURE BY NMR OF 89-117Disulfide folding pathways of cystine knot proteins. Tying the knot within the circular backbone of the cyclotides.STRUCTURE BY NMR OF 89-117Twists, knots, and rings in proteins. Structural definition of the cyclotide framework.STRUCTURE BY NMR OF 89-117Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity.STRUCTURE BY NMR OF 92-115FUNCTIONProbably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.Leaves and stems. Lower in roots.The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight.With oxindolylalanine.With N-formylkynurenine.The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.Belongs to the cyclotide family. Moebius subfamily.The oxidation forms of Trp-111 are subject of controversy and could be the artifactual results of sample handling.The protein with a topological twist - Issue 20 of March 2002Bio-Art - Issue 53 of December 20043D-structureAntibioticAntimicrobialCytolysisDirect protein sequencingDisulfide bondHemolysisKnottinPharmaceuticalPlant defenseSignalMAKFTVCLLLCLLLAAFVGAFGSELSDSHKTTLVNEIAEKMLQRKILDGVEATLVTDVAEKMFLRKMKAEAKTSETADQVFLKQLQLKGLPVCGETCVGGTCNTPGCTCSWPVCTRNGLPSLAA
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