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P56254

- KAB1_OLDAF

UniProt

P56254 - KAB1_OLDAF

Protein

Kalata-B1

Gene

OAK1

Organism
Oldenlandia affinis
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.2 PublicationsPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei111 – 1111Susceptible to oxidation

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. hemolysis in other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial

    Keywords - Biological processi

    Cytolysis, Hemolysis, Plant defense

    Protein family/group databases

    TCDBi9.B.48.1.1. the cyclotide (cyclotide) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kalata-B1
    Gene namesi
    Name:OAK1
    OrganismiOldenlandia affinis
    Taxonomic identifieri60225 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeRubioideaeSpermacoceaeOldenlandia

    Pathology & Biotechi

    Pharmaceutical usei

    The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Propeptidei23 – 8866PRO_0000006617Add
    BLAST
    Peptidei89 – 11729Kalata-B1PRO_0000006618Add
    BLAST
    Propeptidei118 – 1247PRO_0000006619

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki89 ↔ 117Cyclopeptide (Gly-Asn)
    Disulfide bondi93 ↔ 1071 PublicationPROSITE-ProRule annotation
    Disulfide bondi97 ↔ 1091 PublicationPROSITE-ProRule annotation
    Disulfide bondi102 ↔ 1141 PublicationPROSITE-ProRule annotation

    Post-translational modificationi

    Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight.1 Publication

    Keywords - PTMi

    Disulfide bond, Oxidation

    Expressioni

    Tissue specificityi

    Leaves and stems. Lower in roots.

    Structurei

    Secondary structure

    1
    124
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi98 – 1003
    Beta strandi104 – 1063
    Beta strandi108 – 1103
    Beta strandi111 – 1133

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JJZNMR-A89-116[»]
    1K48NMR-A89-116[»]
    1KALNMR-A110-120[»]
    1N1UNMR-A94-120[»]
    1NB1NMR-A93-120[»]
    1ORXNMR-A92-115[»]
    2F2INMR-A93-121[»]
    2F2JNMR-A93-121[»]
    2JUENMR-A94-121[»]
    2KHBNMR-A89-117[»]
    2MH1NMR-A93-120[»]
    ProteinModelPortaliP56254.
    SMRiP56254. Positions 93-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56254.

    Family & Domainsi

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

    Sequence similaritiesi

    Belongs to the cyclotide family. Moebius subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Knottin, Signal

    Family and domain databases

    InterProiIPR005535. Cyclotide.
    IPR012324. Cyclotide_moebius_CS.
    [Graphical view]
    PfamiPF03784. Cyclotide. 1 hit.
    [Graphical view]
    SUPFAMiSSF57038. SSF57038. 1 hit.
    PROSITEiPS51052. CYCLOTIDE. 1 hit.
    PS60009. CYCLOTIDE_MOEBIUS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P56254-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKFTVCLLL CLLLAAFVGA FGSELSDSHK TTLVNEIAEK MLQRKILDGV    50
    EATLVTDVAE KMFLRKMKAE AKTSETADQV FLKQLQLKGL PVCGETCVGG 100
    TCNTPGCTCS WPVCTRNGLP SLAA 124
    Length:124
    Mass (Da):13,271
    Last modified:December 5, 2001 - v3
    Checksum:i4EAD1D69318FCCC9
    GO

    Mass spectrometryi

    Molecular mass is 2892 Da from positions 89 - 117. Determined by ESI. 1 Publication
    Molecular mass is 2982.4 Da from positions 89 - 117. Determined by ESI. 1 Publication
    Molecular mass is 2908.4 Da from positions 89 - 117. Determined by ESI. With oxindolylalanine.1 Publication
    Molecular mass is 2924.4 Da from positions 89 - 117. Determined by ESI. With N-formylkynurenine.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF393825 mRNA. Translation: AAL05477.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The protein with a topological twist - Issue 20 of March 2002

    Protein Spotlight

    Bio-Art - Issue 53 of December 2004

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF393825 mRNA. Translation: AAL05477.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JJZ NMR - A 89-116 [» ]
    1K48 NMR - A 89-116 [» ]
    1KAL NMR - A 110-120 [» ]
    1N1U NMR - A 94-120 [» ]
    1NB1 NMR - A 93-120 [» ]
    1ORX NMR - A 92-115 [» ]
    2F2I NMR - A 93-121 [» ]
    2F2J NMR - A 93-121 [» ]
    2JUE NMR - A 94-121 [» ]
    2KHB NMR - A 89-117 [» ]
    2MH1 NMR - A 93-120 [» ]
    ProteinModelPortali P56254.
    SMRi P56254. Positions 93-120.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 9.B.48.1.1. the cyclotide (cyclotide) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P56254.

    Family and domain databases

    InterProi IPR005535. Cyclotide.
    IPR012324. Cyclotide_moebius_CS.
    [Graphical view ]
    Pfami PF03784. Cyclotide. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57038. SSF57038. 1 hit.
    PROSITEi PS51052. CYCLOTIDE. 1 hit.
    PS60009. CYCLOTIDE_MOEBIUS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis."
      Jennings C.V., West J., Waine C., Craik D.J., Anderson M.A.
      Proc. Natl. Acad. Sci. U.S.A. 98:10614-10619(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Elucidation of the primary and three-dimensional structure of the uterotonic polypeptide kalata B1."
      Saether O., Craik D.J., Campbell I.D., Sletten K., Juul J., Norman D.G.
      Biochemistry 34:4147-4158(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 89-117, MASS SPECTROMETRY, STRUCTURE BY NMR OF 89-109, DISULFIDE BONDS.
    3. "The cyclotide fingerprint in Oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides."
      Plan M.R.R., Goeransson U., Clark R.J., Daly N.L., Colgrave M.L., Craik D.J.
      ChemBioChem 8:1001-1011(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 89-117, FUNCTION, MASS SPECTROMETRY, OXIDATION AT TRP-111.
    4. "An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides."
      Tam J.P., Lu Y.-A., Yang J.-L., Chiu K.-W.
      Proc. Natl. Acad. Sci. U.S.A. 96:8913-8918(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SYNTHESIS OF 89-117, ANTIBACTERIAL ACTIVITY.
    5. "Refined structure and metal binding site of the kalata B1 peptide."
      Skjeldal L., Gran L., Sletten K., Volkman B.F.
      Arch. Biochem. Biophys. 399:142-148(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 89-117.
    6. "Disulfide folding pathways of cystine knot proteins. Tying the knot within the circular backbone of the cyclotides."
      Daly N.L., Clark R.J., Craik D.J.
      J. Biol. Chem. 278:6314-6322(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 89-117.
    7. "Twists, knots, and rings in proteins. Structural definition of the cyclotide framework."
      Rosengren K.J., Daly N.L., Plan M.R.R., Waine C., Craik D.J.
      J. Biol. Chem. 278:8606-8616(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 89-117.
    8. "Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity."
      Barry D.G., Daly N.L., Clark R.J., Sando L., Craik D.J.
      Biochemistry 42:6688-6695(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 92-115, FUNCTION.

    Entry informationi

    Entry nameiKAB1_OLDAF
    AccessioniPrimary (citable) accession number: P56254
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: December 5, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Pharmaceutical

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3