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Protein

Kalata-B1

Gene

OAK1

Organism
Oldenlandia affinis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.PROSITE-ProRule annotation2 Publications

Caution

The oxidation forms of Trp-111 are subject of controversy and could be the artifactual results of sample handling.1 Publication

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial
Biological processCytolysis, Hemolysis, Plant defense

Protein family/group databases

TCDBi9.B.48.1.1 the cyclotide (cyclotide) family

Names & Taxonomyi

Protein namesi
Recommended name:
Kalata-B1
Gene namesi
Name:OAK1
OrganismiOldenlandia affinis
Taxonomic identifieri60225 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeRubioideaeSpermacoceaeHedyotis-Oldenlandia complexOldenlandia

Pathology & Biotechi

Pharmaceutical usei

The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000000661723 – 88Add BLAST66
PeptideiPRO_000000661889 – 117Kalata-B1Add BLAST29
PropeptideiPRO_0000006619118 – 1247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki89 ↔ 117Cyclopeptide (Gly-Asn)
Disulfide bondi93 ↔ 107PROSITE-ProRule annotation1 Publication
Disulfide bondi97 ↔ 109PROSITE-ProRule annotation1 Publication
Disulfide bondi102 ↔ 114PROSITE-ProRule annotation1 Publication

Post-translational modificationi

Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP56254

Expressioni

Tissue specificityi

Leaves and stems. Lower in roots.

Structurei

Secondary structure

1124
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi93 – 96Combined sources4
Beta strandi98 – 100Combined sources3
Beta strandi104 – 106Combined sources3
Beta strandi108 – 110Combined sources3
Beta strandi113 – 116Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JJZNMR-A89-116[»]
1K48NMR-A89-116[»]
1KALNMR-A110-120[»]
1N1UNMR-A94-120[»]
1NB1NMR-A93-120[»]
1ORXNMR-A92-115[»]
2F2INMR-A93-121[»]
2F2JNMR-A93-121[»]
2JUENMR-A94-121[»]
2KHBNMR-A89-117[»]
2MH1NMR-A93-120[»]
2MN1NMR-A89-117[»]
4TTMX-ray1.90A89-117[»]
4TTNX-ray1.25A89-117[»]
4TTOX-ray2.30A89-117[»]
DisProtiDP01017
ProteinModelPortaliP56254
SMRiP56254
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56254

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the cyclotide family. Moebius subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR005535 Cyclotide
IPR012324 Cyclotide_moebius_CS
IPR036146 Cyclotide_sf
PfamiView protein in Pfam
PF03784 Cyclotide, 1 hit
SUPFAMiSSF57038 SSF57038, 1 hit
PROSITEiView protein in PROSITE
PS51052 CYCLOTIDE, 1 hit
PS60009 CYCLOTIDE_MOEBIUS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFTVCLLL CLLLAAFVGA FGSELSDSHK TTLVNEIAEK MLQRKILDGV
60 70 80 90 100
EATLVTDVAE KMFLRKMKAE AKTSETADQV FLKQLQLKGL PVCGETCVGG
110 120
TCNTPGCTCS WPVCTRNGLP SLAA
Length:124
Mass (Da):13,271
Last modified:December 5, 2001 - v3
Checksum:i4EAD1D69318FCCC9
GO

Mass spectrometryi

Molecular mass is 2892 Da from positions 89 - 117. Determined by ESI. 1 Publication
Molecular mass is 2982.4 Da from positions 89 - 117. Determined by ESI. 1 Publication
Molecular mass is 2908.4 Da from positions 89 - 117. Determined by ESI. With oxindolylalanine.1 Publication
Molecular mass is 2924.4 Da from positions 89 - 117. Determined by ESI. With N-formylkynurenine.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393825 mRNA Translation: AAL05477.1

Similar proteinsi

Entry informationi

Entry nameiKAB1_OLDAF
AccessioniPrimary (citable) accession number: P56254
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 5, 2001
Last modified: May 23, 2018
This is version 100 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Pharmaceutical

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