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P56222 (PO3F2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name=Brain-2
Short name=Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name=Oct-7
Octamer-binding transcription factor 7
Short name=OTF-7
Gene names
Name:Pou3f2
Synonyms:Brn2, Otf7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.

Subunit structure

Interacts with PQBP1 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Expressed specifically at high levels in the brain.

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445POU domain, class 3, transcription factor 2
PRO_0000100724

Regions

Domain264 – 33875POU-specific
DNA binding356 – 41560Homeobox
Compositional bias68 – 9023Poly-Gly
Compositional bias125 – 15127Poly-Gln
Compositional bias173 – 1775Poly-Ala
Compositional bias241 – 2477Poly-Pro

Amino acid modifications

Modified residue3431Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P56222 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 9D2729E34E359FD3

FASTA44547,172
        10         20         30         40         50         60 
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL QVQGDYGALQ SNGHPLSHAH 

        70         80         90        100        110        120 
WITALSHGGS GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ PDIKPSVVVQ QGGRGDELHG 

       130        140        150        160        170        180 
PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ QRPPHLVHHA ANNHPGPGAW RSAAAAAHLP 

       190        200        210        220        230        240 
PSMGASNGGL LYSQPSFTVN GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ 

       250        260        270        280        290        300 
PPPPPPPQGP PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL 

       310        320        330        340        350        360 
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI ASQGRKRKKR 

       370        380        390        400        410        420 
TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV RVWFCNRRQK EKRMTPPGGT 

       430        440 
LPGAEDVYGG SRDTPPHHGV QTPVQ 

« Hide

References

[1]"Spacing and orientation of bipartite DNA-binding motifs as potential functional determinants for POU domain factors."
Li P., He X., Gerrero M.R., Mok M., Aggarwal A., Rosenfeld M.G.
Genes Dev. 7:2483-2496(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L27663 mRNA. No translation available.
PIRA49447.
RefSeqNP_742082.1. NM_172085.1.
UniGeneRn.82733.

3D structure databases

ProteinModelPortalP56222.
SMRP56222. Positions 270-416.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000009043.

PTM databases

PhosphoSiteP56222.

Proteomic databases

PaxDbP56222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29588.
KEGGrno:29588.
UCSCRGD:61946. rat.

Organism-specific databases

CTD5454.
RGD61946. Pou3f2.

Phylogenomic databases

eggNOGNOG261729.
HOGENOMHOG000116303.
HOVERGENHBG053120.
InParanoidP56222.
KOK09365.
PhylomeDBP56222.

Gene expression databases

GenevestigatorP56222.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609715.
PROP56222.

Entry information

Entry namePO3F2_RAT
AccessionPrimary (citable) accession number: P56222
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families