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Protein

POU domain, class 3, transcription factor 2

Gene

Pou3f2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (PubMed:8276233). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi356 – 415HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: RGD
  • HMG box domain binding Source: UniProtKB
  • RNA polymerase II transcription coactivator activity Source: UniProtKB
  • sequence-specific DNA binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:Pou3f2
Synonyms:Brn2, Otf7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61946. Pou3f2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007241 – 445POU domain, class 3, transcription factor 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP56222.

PTM databases

PhosphoSitePlusiP56222.

Expressioni

Tissue specificityi

Expressed specifically at high levels in the brain.

Interactioni

Subunit structurei

Interacts with PQBP1. Interaction with ISL1.By similarity

GO - Molecular functioni

  • HMG box domain binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009043.

Structurei

3D structure databases

ProteinModelPortaliP56222.
SMRiP56222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 338POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 90Poly-GlyAdd BLAST23
Compositional biasi125 – 151Poly-GlnAdd BLAST27
Compositional biasi173 – 177Poly-Ala5
Compositional biasi241 – 247Poly-Pro7

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP56222.
PhylomeDBiP56222.

Family and domain databases

InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR016362. TF_POU_3.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P56222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL QVQGDYGALQ
60 70 80 90 100
SNGHPLSHAH WITALSHGGS GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ
160 170 180 190 200
QRPPHLVHHA ANNHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN
210 220 230 240 250
GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP
260 270 280 290 300
PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL
310 320 330 340 350
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI
360 370 380 390 400
ASQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV
410 420 430 440
RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ
Length:445
Mass (Da):47,172
Last modified:July 15, 1998 - v1
Checksum:i9D2729E34E359FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27663 mRNA. No translation available.
PIRiA49447.
UniGeneiRn.82733.

Genome annotation databases

UCSCiRGD:61946. rat.

Similar proteinsi

Entry informationi

Entry nameiPO3F2_RAT
AccessioniPrimary (citable) accession number: P56222
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: August 30, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families