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P56221

- SCYD_MAGO7

UniProt

P56221 - SCYD_MAGO7

Protein

Scytalone dehydratase

Gene

SDH1

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalyzes two steps in melanin biosynthesis. From scytalone they are two dehydration steps and one reduction step to yield melanin.

    Catalytic activityi

    Scytalone = 1,3,8-trihydroxynaphthalene + H2O.

    Pathwayi

    GO - Molecular functioni

    1. scytalone dehydratase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-UniPathway
    2. mycelium development Source: PAMGO_MGG

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Melanin biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00785.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Scytalone dehydratase (EC:4.2.1.94)
    Gene namesi
    Name:SDH1
    ORF Names:MGG_05059
    OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
    Taxonomic identifieri242507 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
    ProteomesiUP000009058: Chromosome 3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 172172Scytalone dehydratasePRO_0000097639Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer. Each subunit contains an active site, located in the central part of the hydrophobic core of the monomer, which functions independently.

    Structurei

    Secondary structure

    1
    172
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 3220
    Helixi35 – 395
    Beta strandi42 – 498
    Helixi51 – 544
    Beta strandi57 – 626
    Helixi63 – 719
    Turni73 – 764
    Beta strandi81 – 833
    Beta strandi86 – 9611
    Beta strandi99 – 11517
    Beta strandi121 – 13818
    Beta strandi141 – 15515
    Helixi158 – 1614
    Helixi163 – 1697

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IDPX-ray1.45A/B/C1-172[»]
    1STDX-ray2.90A1-172[»]
    2STDX-ray2.10A1-172[»]
    3STDX-ray1.65A/B/C10-172[»]
    4STDX-ray2.15A/B/C10-172[»]
    5STDX-ray1.95A/B/C10-172[»]
    6STDX-ray1.80A/B/C10-172[»]
    7STDX-ray1.80A/B/C10-172[»]
    ProteinModelPortaliP56221.
    SMRiP56221. Positions 10-172.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56221.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG135608.
    KOiK17740.
    OrthoDBiEOG7RV9TJ.

    Family and domain databases

    InterProiIPR004235. Scytalone_dehydratase.
    [Graphical view]
    PfamiPF02982. Scytalone_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF024851. SCD1. 1 hit.
    ProDomiPD022193. Scytalone_dehydratase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P56221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSQVQKSDE ITFSDYLGLM TCVYEWADSY DSKDWDRLRK VIAPTLRIDY    50
    RSFLDKLWEA MPAEEFVGMV SSKQVLGDPT LRTQHFIGGT RWEKVSEDEV 100
    IGYHQLRVPH QRYKDTTMKE VTMKGHAHSA NLHWYKKIDG VWKFAGLKPD 150
    IRWGEFDFDR IFEDGRETFG DK 172
    Length:172
    Mass (Da):20,250
    Last modified:July 15, 1998 - v1
    Checksum:i2FA56296D5EE00DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004741 mRNA. Translation: BAA34046.1.
    CM001233 Genomic DNA. Translation: EHA52765.1.
    RefSeqiXP_003712572.1. XM_003712524.1.

    Genome annotation databases

    EnsemblFungiiMGG_05059T0; MGG_05059T0; MGG_05059.
    GeneIDi2675492.
    KEGGimgr:MGG_05059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004741 mRNA. Translation: BAA34046.1 .
    CM001233 Genomic DNA. Translation: EHA52765.1 .
    RefSeqi XP_003712572.1. XM_003712524.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IDP X-ray 1.45 A/B/C 1-172 [» ]
    1STD X-ray 2.90 A 1-172 [» ]
    2STD X-ray 2.10 A 1-172 [» ]
    3STD X-ray 1.65 A/B/C 10-172 [» ]
    4STD X-ray 2.15 A/B/C 10-172 [» ]
    5STD X-ray 1.95 A/B/C 10-172 [» ]
    6STD X-ray 1.80 A/B/C 10-172 [» ]
    7STD X-ray 1.80 A/B/C 10-172 [» ]
    ProteinModelPortali P56221.
    SMRi P56221. Positions 10-172.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P56221.
    ChEMBLi CHEMBL2578.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii MGG_05059T0 ; MGG_05059T0 ; MGG_05059 .
    GeneIDi 2675492.
    KEGGi mgr:MGG_05059.

    Phylogenomic databases

    eggNOGi NOG135608.
    KOi K17740.
    OrthoDBi EOG7RV9TJ.

    Enzyme and pathway databases

    UniPathwayi UPA00785 .

    Miscellaneous databases

    EvolutionaryTracei P56221.

    Family and domain databases

    InterProi IPR004235. Scytalone_dehydratase.
    [Graphical view ]
    Pfami PF02982. Scytalone_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF024851. SCD1. 1 hit.
    ProDomi PD022193. Scytalone_dehydratase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, expression, and mutagenesis of scytalone dehydratase needed for pathogenicity of the rice blast fungus, Pyricularia oryzae."
      Motoyama T., Imanishi K., Yamaguchi I.
      Biosci. Biotechnol. Biochem. 62:564-566(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
    3. "Crystal structure of scytalone dehydratase -- a disease determinant of the rice pathogen, Magnaporthe grisea."
      Lundqvist T., Rice J., Hodge C.N., Basarab G.S., Pierce J., Lindqvist Y.
      Structure 2:937-944(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
    4. "Cryogenic X-ray crystal structure analysis for the complex of scytalone dehydratase of a rice blast fungus and its tight-binding inhibitor, carpropamid: the structural basis of tight-binding inhibition."
      Nakasako M., Motoyama T., Kurahashi Y., Yamaguchi I.
      Biochemistry 37:9931-9939(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    5. "Structure-based design of potent inhibitors of scytalone dehydratase: displacement of a water molecule from the active site."
      Chen J.M., Xu S.L., Wawrzak Z., Basarab G.S., Jordan D.B.
      Biochemistry 37:17735-17744(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
    6. "High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH."
      Wawrzak Z., Sandalova T., Steffens J.J., Basarab G.S., Lundqvist T., Lindqvist Y., Jordan D.B.
      Proteins 35:425-439(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).

    Entry informationi

    Entry nameiSCYD_MAGO7
    AccessioniPrimary (citable) accession number: P56221
    Secondary accession number(s): A4QTI6, G4N445
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3