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Protein

FAD-linked sulfhydryl oxidase ALR

Gene

Gfer

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity).By similarity

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041FADBy similarity
Binding sitei133 – 1331FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 1009FADBy similarity
Nucleotide bindingi164 – 17613FADBy similarityAdd
BLAST
Nucleotide bindingi187 – 1882FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: UniProtKB
  2. protein disulfide oxidoreductase activity Source: UniProtKB
  3. thiol oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_292308. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)
Alternative name(s):
Augmenter of liver regeneration
Gene namesi
Name:Gfer
Synonyms:Alr
ORF Names:MNCb-0663
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107757. Gfer.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. mitochondrial intermembrane space Source: UniProtKB-SubCell
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198FAD-linked sulfhydryl oxidase ALRPRO_0000208549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi88 – 88Interchain (with C-197)PROSITE-ProRule annotation
Disulfide bondi135 ↔ 138Redox-activePROSITE-ProRule annotation
Disulfide bondi164 ↔ 181PROSITE-ProRule annotation
Disulfide bondi197 – 197Interchain (with C-88)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP56213.
PaxDbiP56213.
PRIDEiP56213.

PTM databases

PhosphoSiteiP56213.

Expressioni

Tissue specificityi

Preferentially expressed in the liver and in testis.1 Publication

Gene expression databases

BgeeiP56213.
GenevestigatoriP56213.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP56213. 2 interactions.
MINTiMINT-4129999.

Structurei

3D structure databases

ProteinModelPortaliP56213.
SMRiP56213. Positions 86-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 188101ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5054.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
HOVERGENiHBG000235.
InParanoidiP56213.
KOiK17783.
OMAiMDDLATD.
OrthoDBiEOG7HMS2X.
PhylomeDBiP56213.
TreeFamiTF105271.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSEPAGF PRGSRFSFLP GGARSEMTDD LVTDARGRGA RHRDDTTPAA
60 70 80 90 100
APAPQGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRHTW
110 120 130 140 150
AFLHTLAAYY PDRPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIGRNQ
160 170 180 190
PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
Length:198
Mass (Da):22,877
Last modified:December 15, 2008 - v2
Checksum:i4828C5D5B2F61054
GO

Sequence cautioni

The sequence AAD36987.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401A → P in AAD10339 (PubMed:8900538).Curated
Sequence conflicti49 – 491A → S in AAD10339 (PubMed:8900538).Curated
Sequence conflicti49 – 491A → S in AAH23941 (PubMed:15489334).Curated
Sequence conflicti49 – 491A → S in AAD36987 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40494 Genomic DNA. Translation: AAD10339.1.
AB041561 mRNA. Translation: BAA95045.1.
AK146579 mRNA. Translation: BAE27275.1.
CH466606 Genomic DNA. Translation: EDL22354.1.
BC023941 mRNA. Translation: AAH23941.1.
AF148688 mRNA. Translation: AAD36987.1. Different initiation.
CCDSiCCDS28492.1.
RefSeqiNP_075527.2. NM_023040.3.
UniGeneiMm.28124.

Genome annotation databases

EnsembliENSMUST00000046839; ENSMUSP00000049186; ENSMUSG00000040888.
GeneIDi11692.
KEGGimmu:11692.
UCSCiuc008axr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40494 Genomic DNA. Translation: AAD10339.1.
AB041561 mRNA. Translation: BAA95045.1.
AK146579 mRNA. Translation: BAE27275.1.
CH466606 Genomic DNA. Translation: EDL22354.1.
BC023941 mRNA. Translation: AAH23941.1.
AF148688 mRNA. Translation: AAD36987.1. Different initiation.
CCDSiCCDS28492.1.
RefSeqiNP_075527.2. NM_023040.3.
UniGeneiMm.28124.

3D structure databases

ProteinModelPortaliP56213.
SMRiP56213. Positions 86-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56213. 2 interactions.
MINTiMINT-4129999.

PTM databases

PhosphoSiteiP56213.

Proteomic databases

MaxQBiP56213.
PaxDbiP56213.
PRIDEiP56213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046839; ENSMUSP00000049186; ENSMUSG00000040888.
GeneIDi11692.
KEGGimmu:11692.
UCSCiuc008axr.1. mouse.

Organism-specific databases

CTDi2671.
MGIiMGI:107757. Gfer.

Phylogenomic databases

eggNOGiCOG5054.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
HOVERGENiHBG000235.
InParanoidiP56213.
KOiK17783.
OMAiMDDLATD.
OrthoDBiEOG7HMS2X.
PhylomeDBiP56213.
TreeFamiTF105271.

Enzyme and pathway databases

ReactomeiREACT_292308. Mitochondrial protein import.

Miscellaneous databases

NextBioi279349.
PROiP56213.
SOURCEiSearch...

Gene expression databases

BgeeiP56213.
GenevestigatoriP56213.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the structure and expression of the augmenter of liver regeneration (ALR) gene."
    Giorda R., Hagiya M., Seki T., Shimonishi M., Sakai H., Michaelson J., Francavilla A., Starzl T.E., Trucco M.
    Mol. Med. 2:97-108(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: C57BL/6 X CBA.
  2. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  6. "Cloning and sequence analysis of a mouse cDNA coding for augmenter of liver regeneration."
    Cheng J., Zhong Y.W., Liu Y., Dong J., Yang J.Z., Chen J.M.
    Zhonghua Gan Zang Bing Za Zhi 4:138-140(1998)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-198.
    Strain: C57BL/6 X CBA.

Entry informationi

Entry nameiALR_MOUSE
AccessioniPrimary (citable) accession number: P56213
Secondary accession number(s): Q8CIF8, Q9JJE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1998
Last sequence update: December 15, 2008
Last modified: March 31, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.