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Protein

FAD-linked sulfhydryl oxidase ALR

Gene

Gfer

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity).By similarity

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104FADBy similarity1
Binding sitei133FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 100FADBy similarity9
Nucleotide bindingi164 – 176FADBy similarityAdd BLAST13
Nucleotide bindingi187 – 188FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)
Alternative name(s):
Augmenter of liver regeneration
Gene namesi
Name:Gfer
Synonyms:Alr
ORF Names:MNCb-0663
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107757. Gfer.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085491 – 198FAD-linked sulfhydryl oxidase ALRAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi88Interchain (with C-197)PROSITE-ProRule annotation
Disulfide bondi135 ↔ 138Redox-activePROSITE-ProRule annotation
Disulfide bondi164 ↔ 181PROSITE-ProRule annotation
Disulfide bondi197Interchain (with C-88)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP56213.
MaxQBiP56213.
PaxDbiP56213.
PeptideAtlasiP56213.
PRIDEiP56213.

PTM databases

iPTMnetiP56213.
PhosphoSitePlusiP56213.
SwissPalmiP56213.

Expressioni

Tissue specificityi

Preferentially expressed in the liver and in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000040888.
GenevisibleiP56213. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP56213. 2 interactors.
MINTiMINT-4129999.
STRINGi10090.ENSMUSP00000049186.

Structurei

3D structure databases

ProteinModelPortaliP56213.
SMRiP56213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 188ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3355. Eukaryota.
COG5054. LUCA.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
HOVERGENiHBG000235.
InParanoidiP56213.
KOiK17783.
OMAiHGGNLFF.
OrthoDBiEOG091G0TRW.
PhylomeDBiP56213.
TreeFamiTF105271.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSEPAGF PRGSRFSFLP GGARSEMTDD LVTDARGRGA RHRDDTTPAA
60 70 80 90 100
APAPQGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRHTW
110 120 130 140 150
AFLHTLAAYY PDRPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIGRNQ
160 170 180 190
PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
Length:198
Mass (Da):22,877
Last modified:December 16, 2008 - v2
Checksum:i4828C5D5B2F61054
GO

Sequence cautioni

The sequence AAD36987 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40A → P in AAD10339 (PubMed:8900538).Curated1
Sequence conflicti49A → S in AAD10339 (PubMed:8900538).Curated1
Sequence conflicti49A → S in AAH23941 (PubMed:15489334).Curated1
Sequence conflicti49A → S in AAD36987 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40494 Genomic DNA. Translation: AAD10339.1.
AB041561 mRNA. Translation: BAA95045.1.
AK146579 mRNA. Translation: BAE27275.1.
CH466606 Genomic DNA. Translation: EDL22354.1.
BC023941 mRNA. Translation: AAH23941.1.
AF148688 mRNA. Translation: AAD36987.1. Different initiation.
CCDSiCCDS28492.1.
RefSeqiNP_075527.2. NM_023040.3.
UniGeneiMm.28124.

Genome annotation databases

EnsembliENSMUST00000046839; ENSMUSP00000049186; ENSMUSG00000040888.
GeneIDi11692.
KEGGimmu:11692.
UCSCiuc008axr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40494 Genomic DNA. Translation: AAD10339.1.
AB041561 mRNA. Translation: BAA95045.1.
AK146579 mRNA. Translation: BAE27275.1.
CH466606 Genomic DNA. Translation: EDL22354.1.
BC023941 mRNA. Translation: AAH23941.1.
AF148688 mRNA. Translation: AAD36987.1. Different initiation.
CCDSiCCDS28492.1.
RefSeqiNP_075527.2. NM_023040.3.
UniGeneiMm.28124.

3D structure databases

ProteinModelPortaliP56213.
SMRiP56213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56213. 2 interactors.
MINTiMINT-4129999.
STRINGi10090.ENSMUSP00000049186.

PTM databases

iPTMnetiP56213.
PhosphoSitePlusiP56213.
SwissPalmiP56213.

Proteomic databases

EPDiP56213.
MaxQBiP56213.
PaxDbiP56213.
PeptideAtlasiP56213.
PRIDEiP56213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046839; ENSMUSP00000049186; ENSMUSG00000040888.
GeneIDi11692.
KEGGimmu:11692.
UCSCiuc008axr.1. mouse.

Organism-specific databases

CTDi2671.
MGIiMGI:107757. Gfer.

Phylogenomic databases

eggNOGiKOG3355. Eukaryota.
COG5054. LUCA.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
HOVERGENiHBG000235.
InParanoidiP56213.
KOiK17783.
OMAiHGGNLFF.
OrthoDBiEOG091G0TRW.
PhylomeDBiP56213.
TreeFamiTF105271.

Miscellaneous databases

PROiP56213.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040888.
GenevisibleiP56213. MM.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALR_MOUSE
AccessioniPrimary (citable) accession number: P56213
Secondary accession number(s): Q8CIF8, Q9JJE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.