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Protein

Omega-hexatoxin-Hv1a

Gene
N/A
Organism
Hadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly and voltage-independently blocks both mid-low- (M-LVA) and high-voltage-activated (HVA) calcium channels in cockroach DUM neurons. Lethal to many insect orders but not toxic to mice or rabbits. May target the insect high-voltage-activated calcium channel Dmca1D. Also inhibits acarines calcium channels. An extremely high toxin concentration partially inhibits Cav1.2/CACNA1C, Cav2.1/CACNA1A and Cav2.2/CACNA1B calcium channel of rats. As for omega-AcTx-Hv2a, the phenotypic effect of injection of this toxin into lone star ticks (Amblyomma americanum) is curling of all eight legs into closed loops.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei10Critical for insecticidal activity1
Sitei27Critical for insecticidal activity1
Sitei35Critical for insecticidal activity1

GO - Molecular functioni

  • calcium channel inhibitor activity Source: CACAO

GO - Biological processi

Keywordsi

Molecular functionCalcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-hexatoxin-Hv1a
Short name:
Omega-HXTX-Hv1a
Alternative name(s):
Omega-atracotoxin-Hv1a
Short name:
AcTx-Hv1
Short name:
Omega-AcTx-Hv1a
OrganismiHadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Taxonomic identifieri6904 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeHexathelidaeHadronyche

Organism-specific databases

ArachnoServeriAS000193. omega-hexatoxin-Hv1a.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

PD50 is 3 µmol/kg when injected into tobacco budworm (Heliothis armigera). PD50 is 0.25 µmol/kg when injected into tobacco budworm (Heliothis virescens).
LD50 is 89-91 pmol/g (0.38 mg/kg) when injected into house crickets (Acheta domestica). LD50 is 86.5 pmol/g when injected into house flies (Musca domestica).
LD50 is 716 +-23 pmol/g (3.00 +-0.10 µg/g) when orally administered into lone star ticks (Amblyomma americanum). LD50 is 447 +-3 pmol/g (1877 +-0.013 µg/g) when injected into lone star ticks (Amblyomma americanum).

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9Q → A: 4-fold decrease in toxicity to crickets, and insignificant decrease to flies. 16-fold decrease in binding affinity to cockroach membranes. 1 Publication1
Mutagenesisi10P → A: 6-fold decrease in toxicity to crickets and 15-fold to flies. 190-fold decrease in binding affinity to cockroach membranes. 1 Publication1
Mutagenesisi13Y → A: 16-fold decrease in binding affinity to cockroach membranes. 1
Mutagenesisi27N → A: 14-fold decrease in toxicity to crickets and 30-fold to flies. Abolishes toxicity to crickets and flies; when associated with A-35. 195-fold decrease in binding affinity to cockroach membranes. 2 Publications1
Mutagenesisi27N → D: 12-fold decrease in toxicity to crickets. 2 Publications1
Mutagenesisi35R → A: 4.4-fold decrease in toxicity to crickets, and 15 to flies. Abolishes toxicity to crickets and flies; when associated with A-27. 280-fold decrease in binding affinity to cockroach membranes. 2 Publications1
Mutagenesisi35R → E: 7.8-fold decrease in toxicity to crickets. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000449881 – 37Omega-hexatoxin-Hv1aAdd BLAST37

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi4 ↔ 18
Disulfide bondi11 ↔ 22
Disulfide bondi17 ↔ 36

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 16Combined sources3
Beta strandi17 – 20Combined sources4
Beta strandi22 – 26Combined sources5
Beta strandi28 – 30Combined sources3
Beta strandi32 – 36Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXHNMR-A1-37[»]
1HVWNMR-A4-37[»]
ProteinModelPortaliP56207.
SMRiP56207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56207.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the Shiva superfamily. Omega toxin family.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiView protein in InterPro
IPR009415. Omega-atracotox.
IPR018071. Omega-atracotox_CS.
PfamiView protein in Pfam
PF06357. Omega-toxin. 1 hit.
PROSITEiView protein in PROSITE
PS60016. OMEGA_ACTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P56207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
SPTCIPSGQP CPYNENCCSQ SCTFKENENG NTVKRCD
Length:37
Mass (Da):4,055
Last modified:November 1, 1997 - v1
Checksum:iA115058BA4364D2F
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXHNMR-A1-37[»]
1HVWNMR-A4-37[»]
ProteinModelPortaliP56207.
SMRiP56207.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000193. omega-hexatoxin-Hv1a.

Miscellaneous databases

EvolutionaryTraceiP56207.

Family and domain databases

InterProiView protein in InterPro
IPR009415. Omega-atracotox.
IPR018071. Omega-atracotox_CS.
PfamiView protein in Pfam
PF06357. Omega-toxin. 1 hit.
PROSITEiView protein in PROSITE
PS60016. OMEGA_ACTX_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTO1A_HADVE
AccessioniPrimary (citable) accession number: P56207
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

When expressed in tobacco plants, the toxin induces a 100% mortality of insect larvae H.armigera and S.littoralis.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.