Reviewed, UniProtKB/Swiss-Prot P56201 (G6PE_RABIT)

Names and origin

Protein names

Recommended name:
    GDH/6PGL endoplasmic bifunctional protein
Including the following 2 domains:
    1- Recommended name:
            Glucose 1-dehydrogenase
              EC=1.1.1.47
        Alternative name(s):
            Hexose-6-phosphate dehydrogenase
    2- Recommended name:
            6-phosphogluconolactonase
                Short name=6PGL
              EC=3.1.1.31

Gene names

Name:	H6PD
Synonyms:	GDH

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates By similarity.
Catalytic activity	Beta-D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H. D-glucose 6-phosphate + NAD(P)(+) = D-glucono-1,5-lactone 6-phosphate + NAD(P)H. 6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate.
Subcellular location	Endoplasmic reticulum lumen. Note= Microsomes, endoplasmic reticulum lumen.
Sequence similarities	In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family. In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
Sequence caution	The sequence described in Ref.1 differs from that shown. Reason: Miscellaneous discrepancy. The sequence was incorrect due to the transposition of a section that was put in position 330 to 436 and which is now transposed to position 593 to 700.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Endoplasmic reticulum
Ligand	NAD NADP
Molecular function	Hydrolase Oxidoreductase
PTM	Glycoprotein Phosphoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	6-phosphogluconolactonase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro glucose 1-dehydrogenase activity Inferred from electronic annotation. Source: EC glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	GDH/6PGL endoplasmic bifunctional protein		PRO_0000068139
Regions
	Region	1 – 507	507	Glucose 1-dehydrogenase
	Region	508 – 521	14	Linker
	Region	522 – 763	242	6-phosphogluconolactonase
Sites
	Active site	248	1	Proton acceptor By similarity
	Binding site	15	1	NADP By similarity
	Binding site	47	1	NADP By similarity
	Binding site	185	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid
	Modified residue	297	1	Phosphotyrosine By similarity
	Glycosylation	138	1	N-linked (GlcNAc...)
	Glycosylation	263	1	N-linked (GlcNAc...)
Natural variations
	Natural variant	69	1	R → K
	Natural variant	75	1	L → R

Sequences

	Sequence		Length	Mass (Da)
	P56201-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 2A4E1039062E8672	FASTA	763	85,285
	10 20 30 40 50 60 QELQGHVSVI LLGATGDLAK KYLWQGLFQL FLDEAGKGHS FSFHGAALTA PKQGQELMAK 70 80 90 100 110 120 ALESLSCPRD MAPSLCAELQ AQFLRLSRYR QLKTAEDYQA LGRDIEAQVQ QEGLREAGRM 130 140 150 160 170 180 FYFSVPPFAY ADIARNINSS CRPGPGAWLR VVLEKPFGHD HLSAQQLATE LGSFFQEEEM 190 200 210 220 230 240 YRVDHYLGKQ AVAQILPFRD QNRRALDSLW NRHHVERVEI IMKETVDAEG RTSFYEEYGV 250 260 270 280 290 300 IRDTLQNHLT EILTLVAMEL PANVSCSEAV LRHKLQAFRA LRRLQRGSAV VGQYQTYSEQ 310 320 330 340 350 360 VRGELRKPAG SPSLTPTFAG VLVHVDNLRM EGVPFILMSG KALDERVGYV RVLFKNQAFC 370 380 390 400 410 420 AQSEKHWAPA QSRCLPRCII FYIGHGELGH PAVVVSRNLF RPFLPAQSWR EVEDRPGLQL 430 440 450 460 470 480 FGRPLSDFYA FSPVKERDAY SILLSHIFHA RKESFVPTEH LLASWVFWTP LLESLAREVP 490 500 510 520 530 540 RLYPGGADSG RLLDFEFSGS HLSFSLGQPE QLVPGPGSTP RPSDFQVLGA KYRESPLISA 550 560 570 580 590 600 WPDELISKLA SDIEAAAVQA VRRVGTFHLA LSGGSSPIAL FQQLASGHYG FPVPLSDPES 610 620 630 640 650 660 NFQGLQAHLL QHVRVPYYNI HPMPVNLHQR LCAEEDRGAQ AAYASEISAL VTWCSFDEVL 670 680 690 700 710 720 QGMGTDGHTA SLFPQSPTGL DGEQLVVLTE SPSRPHQQRR MSLSLPLINR AKKVAVLVMG 730 740 750 760 RTKRDITLLV SRVGREPKNW PISGVLPTSG QLVWYMDYEA FLG

References

[1]	"Isolation and the complete amino acid sequence of lumenal endoplasmic reticulum glucose-6-phosphate dehydrogenase." Ozols J. Proc. Natl. Acad. Sci. U.S.A. 90:5302-5306(1993) [PubMed: 8506377] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: New Zealand white. Tissue: Liver.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Genome annotation databases
Ensembl	ENSOCUG00000000798. Oryctolagus cuniculus. [Contig view]
Phylogenomic databases
HOVERGEN	P56201.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. IPR005900. Phosphogluconlac. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01198. pgl. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PE_RABIT

Accession

Primary (citable) accession number: P56201

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	November 25, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)