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P56200

- T2CX_CITFR

UniProt

P56200 - T2CX_CITFR

Protein

Type-2 restriction enzyme Cfr10I

Gene

cfr10IR

Organism
Citrobacter freundii
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence RCCGGY and cleaves after R-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Binds 2 magnesium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Magnesium 1By similarity
    Metal bindingi134 – 1341Magnesium 2By similarity
    Metal bindingi204 – 2041Magnesium 1Curated

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme Cfr10I (EC:3.1.21.4)
    Short name:
    R.Cfr10I
    Alternative name(s):
    Endonuclease Cfr10I
    Type II restriction enzyme Cfr10I
    Gene namesi
    Name:cfr10IR
    OrganismiCitrobacter freundii
    Taxonomic identifieri546 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Type-2 restriction enzyme Cfr10IPRO_0000077295Add
    BLAST

    Structurei

    Secondary structure

    1
    285
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Beta strandi12 – 154
    Helixi17 – 2610
    Turni30 – 323
    Helixi35 – 5319
    Helixi59 – 8426
    Beta strandi88 – 936
    Turni97 – 993
    Helixi102 – 1054
    Helixi108 – 12316
    Beta strandi134 – 1396
    Helixi144 – 1507
    Turni151 – 1533
    Helixi161 – 1699
    Helixi170 – 1745
    Turni180 – 1823
    Beta strandi183 – 1897
    Helixi195 – 21824
    Beta strandi228 – 2358
    Helixi238 – 2447
    Helixi249 – 2524
    Beta strandi261 – 2688
    Helixi272 – 28211

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CFRX-ray2.15A1-285[»]
    ProteinModelPortaliP56200.
    SMRiP56200. Positions 1-283.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56200.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.91.10. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
    IPR012415. Restrct_endonuc_II_Cfr10I.
    [Graphical view]
    PfamiPF07832. Bse634I. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P56200-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIISKSGEG NKYTINSAIA FVAYASHIDI NTTEFSKVLS GLRDFINDEA    50
    IRLGGKISDG SFNKCNGDWY EWLIGIRAIE FFLESETNFI VVKMPNATSF 100
    DVMSIYKSCL SEFIYDLRSK LSLNNVNLIT SNPDFSIIDI RGRREELKSM 150
    LKDISFSNIS LSTISEIDNL YKNFIDYAEL EHIKSFLSVK TTFRPDRRLQ 200
    LAHEGSLMKA LYTHLQTRTW TINPTGIRYY AAATSIGNAD VIGLKTVATH 250
    SITDVKSLPQ SAVDEIFKIN SVLDVDSCLS HILSS 285
    Length:285
    Mass (Da):32,016
    Last modified:November 1, 1997 - v1
    Checksum:i5F95F49ED058D743
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CFR X-ray 2.15 A 1-285 [» ]
    ProteinModelPortali P56200.
    SMRi P56200. Positions 1-283.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P56200.

    Family and domain databases

    Gene3Di 3.40.91.10. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
    IPR012415. Restrct_endonuc_II_Cfr10I.
    [Graphical view ]
    Pfami PF07832. Bse634I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15-A resolution."
      Bozic D., Grazulis S., Siksnys V., Huber R.
      J. Mol. Biol. 255:176-186(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
      Strain: RFL10.

    Entry informationi

    Entry nameiT2CX_CITFR
    AccessioniPrimary (citable) accession number: P56200
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3