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P56200 (T2CX_CITFR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme Cfr10I

Short name=R.Cfr10I
EC=3.1.21.4
Alternative name(s):
Endonuclease Cfr10I
Type II restriction enzyme Cfr10I
Gene names
Name:cfr10IR
OrganismCitrobacter freundii
Taxonomic identifier546 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence RCCGGY and cleaves after R-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Binds 2 magnesium ions per subunit.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Type-2 restriction enzyme Cfr10I
PRO_0000077295

Sites

Metal binding1341Magnesium 1 By similarity
Metal binding1341Magnesium 2 By similarity
Metal binding2041Magnesium 1 Probable

Secondary structure

............................................ 285
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56200 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 5F95F49ED058D743

FASTA28532,016
        10         20         30         40         50         60 
MDIISKSGEG NKYTINSAIA FVAYASHIDI NTTEFSKVLS GLRDFINDEA IRLGGKISDG 

        70         80         90        100        110        120 
SFNKCNGDWY EWLIGIRAIE FFLESETNFI VVKMPNATSF DVMSIYKSCL SEFIYDLRSK 

       130        140        150        160        170        180 
LSLNNVNLIT SNPDFSIIDI RGRREELKSM LKDISFSNIS LSTISEIDNL YKNFIDYAEL 

       190        200        210        220        230        240 
EHIKSFLSVK TTFRPDRRLQ LAHEGSLMKA LYTHLQTRTW TINPTGIRYY AAATSIGNAD 

       250        260        270        280 
VIGLKTVATH SITDVKSLPQ SAVDEIFKIN SVLDVDSCLS HILSS 

« Hide

References

[1]"Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15-A resolution."
Bozic D., Grazulis S., Siksnys V., Huber R.
J. Mol. Biol. 255:176-186(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
Strain: RFL10.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CFRX-ray2.15A1-285[»]
ProteinModelPortalP56200.
SMRP56200. Positions 1-283.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.91.10. 1 hit.
InterProIPR011335. Restrct_endonuc-II-like.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
IPR012415. Restrct_endonuc_II_Cfr10I.
[Graphical view]
PfamPF07832. Bse634I. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP56200.

Entry information

Entry nameT2CX_CITFR
AccessionPrimary (citable) accession number: P56200
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references