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P56200

- T2CX_CITFR

UniProt

P56200 - T2CX_CITFR

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Protein

Type-2 restriction enzyme Cfr10I

Gene
cfr10IR
Organism
Citrobacter freundii
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence RCCGGY and cleaves after R-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Magnesium 1 By similarity
Metal bindingi134 – 1341Magnesium 2 By similarity
Metal bindingi204 – 2041Magnesium 1 Inferred

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Cfr10I (EC:3.1.21.4)
Short name:
R.Cfr10I
Alternative name(s):
Endonuclease Cfr10I
Type II restriction enzyme Cfr10I
Gene namesi
Name:cfr10IR
OrganismiCitrobacter freundii
Taxonomic identifieri546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Type-2 restriction enzyme Cfr10IPRO_0000077295Add
BLAST

Structurei

Secondary structure

1
285
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64
Beta strandi12 – 154
Helixi17 – 2610
Turni30 – 323
Helixi35 – 5319
Helixi59 – 8426
Beta strandi88 – 936
Turni97 – 993
Helixi102 – 1054
Helixi108 – 12316
Beta strandi134 – 1396
Helixi144 – 1507
Turni151 – 1533
Helixi161 – 1699
Helixi170 – 1745
Turni180 – 1823
Beta strandi183 – 1897
Helixi195 – 21824
Beta strandi228 – 2358
Helixi238 – 2447
Helixi249 – 2524
Beta strandi261 – 2688
Helixi272 – 28211

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CFRX-ray2.15A1-285[»]
ProteinModelPortaliP56200.
SMRiP56200. Positions 1-283.

Miscellaneous databases

EvolutionaryTraceiP56200.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
IPR012415. Restrct_endonuc_II_Cfr10I.
[Graphical view]
PfamiPF07832. Bse634I. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P56200-1 [UniParc]FASTAAdd to Basket

« Hide

MDIISKSGEG NKYTINSAIA FVAYASHIDI NTTEFSKVLS GLRDFINDEA    50
IRLGGKISDG SFNKCNGDWY EWLIGIRAIE FFLESETNFI VVKMPNATSF 100
DVMSIYKSCL SEFIYDLRSK LSLNNVNLIT SNPDFSIIDI RGRREELKSM 150
LKDISFSNIS LSTISEIDNL YKNFIDYAEL EHIKSFLSVK TTFRPDRRLQ 200
LAHEGSLMKA LYTHLQTRTW TINPTGIRYY AAATSIGNAD VIGLKTVATH 250
SITDVKSLPQ SAVDEIFKIN SVLDVDSCLS HILSS 285
Length:285
Mass (Da):32,016
Last modified:November 1, 1997 - v1
Checksum:i5F95F49ED058D743
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CFR X-ray 2.15 A 1-285 [» ]
ProteinModelPortali P56200.
SMRi P56200. Positions 1-283.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P56200.

Family and domain databases

Gene3Di 3.40.91.10. 1 hit.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
IPR012415. Restrct_endonuc_II_Cfr10I.
[Graphical view ]
Pfami PF07832. Bse634I. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15-A resolution."
    Bozic D., Grazulis S., Siksnys V., Huber R.
    J. Mol. Biol. 255:176-186(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
    Strain: RFL10.

Entry informationi

Entry nameiT2CX_CITFR
AccessioniPrimary (citable) accession number: P56200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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