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Protein

Histidine--tRNA ligase

Gene

hisS

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-742-MONOMER.
BRENDAi6.1.1.21. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:hisS
Ordered Locus Names:TTHA0712
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Histidine--tRNA ligasePRO_0000136284Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi300852.TTHA0712.

Structurei

Secondary structure

1
421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3320Combined sources
Beta strandi37 – 404Combined sources
Beta strandi43 – 464Combined sources
Helixi47 – 548Combined sources
Helixi59 – 635Combined sources
Beta strandi67 – 693Combined sources
Beta strandi75 – 784Combined sources
Helixi83 – 9210Combined sources
Helixi95 – 973Combined sources
Beta strandi98 – 11114Combined sources
Beta strandi122 – 13312Combined sources
Helixi137 – 15317Combined sources
Beta strandi160 – 1656Combined sources
Helixi169 – 18315Combined sources
Helixi184 – 1896Combined sources
Helixi192 – 1976Combined sources
Helixi202 – 2054Combined sources
Helixi211 – 22010Combined sources
Helixi225 – 2284Combined sources
Helixi231 – 24616Combined sources
Beta strandi251 – 2533Combined sources
Beta strandi266 – 2727Combined sources
Beta strandi274 – 2785Combined sources
Beta strandi281 – 2877Combined sources
Helixi291 – 2944Combined sources
Beta strandi302 – 3087Combined sources
Helixi309 – 31810Combined sources
Beta strandi330 – 3378Combined sources
Helixi338 – 35114Combined sources
Turni352 – 3543Combined sources
Beta strandi357 – 3593Combined sources
Helixi366 – 37510Combined sources
Beta strandi379 – 3846Combined sources
Helixi386 – 3916Combined sources
Beta strandi393 – 3986Combined sources
Turni399 – 4013Combined sources
Beta strandi404 – 4085Combined sources
Helixi411 – 42010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ADJX-ray2.70A/B/C/D1-421[»]
1ADYX-ray2.80A/B/C/D1-421[»]
ProteinModelPortaliP56194.
SMRiP56194. Positions 2-421.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56194.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 171171CatalyticAdd
BLAST
Regioni229 – 32092CatalyticAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CUT. Bacteria.
COG0124. LUCA.
HOGENOMiHOG000018072.
KOiK01892.
OMAiCGGGNFK.
PhylomeDBiP56194.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARAVRGTK DLFGKELRMH QRIVATARKV LEAAGALELV TPIFEETQVF
60 70 80 90 100
EKGVGAATDI VRKEMFTFQD RGGRSLTLRP EGTAAMVRAY LEHGMKVWPQ
110 120 130 140 150
PVRLWMAGPM FRAERPQKGR YRQFHQVNYE ALGSENPILD AEAVVLLYEC
160 170 180 190 200
LKELGLRRLK VKLSSVGDPE DRARYNAYLR EVLSPHREAL SEDSKERLEL
210 220 230 240 250
NPMRILDSKS ERDQALLKEL GVRPMLDFLG EEARAHLKEV ERHLERLSVP
260 270 280 290 300
YELEPALVRG LDYYVRTAFE VHHEEIGAQS ALGGGGRYDG LSELLGGPRV
310 320 330 340 350
PGVGFAFGVE RVALALEAEG FGLPEEKGPD LYLIPLTEEA VAEAFYLAEA
360 370 380 390 400
LRPRLRAEYA LAPRKPAKGL EEALKRGAAF AGFLGEDELR AGEVTLKRLA
410 420
TGEQVRLSRE EVPGYLLQAL G
Length:421
Mass (Da):47,041
Last modified:March 29, 2005 - v2
Checksum:i9DEEE25F2C570A27
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001L → E (PubMed:9115984).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70535.1.
RefSeqiWP_011172810.1. NC_006461.1.
YP_143978.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70535; BAD70535; BAD70535.
GeneIDi3168691.
KEGGittj:TTHA0712.
PATRICi23956401. VBITheThe93045_0706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70535.1.
RefSeqiWP_011172810.1. NC_006461.1.
YP_143978.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ADJX-ray2.70A/B/C/D1-421[»]
1ADYX-ray2.80A/B/C/D1-421[»]
ProteinModelPortaliP56194.
SMRiP56194. Positions 2-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70535; BAD70535; BAD70535.
GeneIDi3168691.
KEGGittj:TTHA0712.
PATRICi23956401. VBITheThe93045_0706.

Phylogenomic databases

eggNOGiENOG4105CUT. Bacteria.
COG0124. LUCA.
HOGENOMiHOG000018072.
KOiK01892.
OMAiCGGGNFK.
PhylomeDBiP56194.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-742-MONOMER.
BRENDAi6.1.1.21. 2305.

Miscellaneous databases

EvolutionaryTraceiP56194.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYH_THET8
AccessioniPrimary (citable) accession number: P56194
Secondary accession number(s): Q5SKD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.