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Protein

Histidine--tRNA ligase

Gene

hisS

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.21. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:hisS
Ordered Locus Names:TTHA0712
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001362841 – 421Histidine--tRNA ligaseAdd BLAST421

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi300852.TTHA0712.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 33Combined sources20
Beta strandi37 – 40Combined sources4
Beta strandi43 – 46Combined sources4
Helixi47 – 54Combined sources8
Helixi59 – 63Combined sources5
Beta strandi67 – 69Combined sources3
Beta strandi75 – 78Combined sources4
Helixi83 – 92Combined sources10
Helixi95 – 97Combined sources3
Beta strandi98 – 111Combined sources14
Beta strandi122 – 133Combined sources12
Helixi137 – 153Combined sources17
Beta strandi160 – 165Combined sources6
Helixi169 – 183Combined sources15
Helixi184 – 189Combined sources6
Helixi192 – 197Combined sources6
Helixi202 – 205Combined sources4
Helixi211 – 220Combined sources10
Helixi225 – 228Combined sources4
Helixi231 – 246Combined sources16
Beta strandi251 – 253Combined sources3
Beta strandi266 – 272Combined sources7
Beta strandi274 – 278Combined sources5
Beta strandi281 – 287Combined sources7
Helixi291 – 294Combined sources4
Beta strandi302 – 308Combined sources7
Helixi309 – 318Combined sources10
Beta strandi330 – 337Combined sources8
Helixi338 – 351Combined sources14
Turni352 – 354Combined sources3
Beta strandi357 – 359Combined sources3
Helixi366 – 375Combined sources10
Beta strandi379 – 384Combined sources6
Helixi386 – 391Combined sources6
Beta strandi393 – 398Combined sources6
Turni399 – 401Combined sources3
Beta strandi404 – 408Combined sources5
Helixi411 – 420Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ADJX-ray2.70A/B/C/D1-421[»]
1ADYX-ray2.80A/B/C/D1-421[»]
ProteinModelPortaliP56194.
SMRiP56194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56194.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 171CatalyticAdd BLAST171
Regioni229 – 320CatalyticAdd BLAST92

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CUT. Bacteria.
COG0124. LUCA.
HOGENOMiHOG000018072.
KOiK01892.
OMAiCGGGNFK.
PhylomeDBiP56194.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARAVRGTK DLFGKELRMH QRIVATARKV LEAAGALELV TPIFEETQVF
60 70 80 90 100
EKGVGAATDI VRKEMFTFQD RGGRSLTLRP EGTAAMVRAY LEHGMKVWPQ
110 120 130 140 150
PVRLWMAGPM FRAERPQKGR YRQFHQVNYE ALGSENPILD AEAVVLLYEC
160 170 180 190 200
LKELGLRRLK VKLSSVGDPE DRARYNAYLR EVLSPHREAL SEDSKERLEL
210 220 230 240 250
NPMRILDSKS ERDQALLKEL GVRPMLDFLG EEARAHLKEV ERHLERLSVP
260 270 280 290 300
YELEPALVRG LDYYVRTAFE VHHEEIGAQS ALGGGGRYDG LSELLGGPRV
310 320 330 340 350
PGVGFAFGVE RVALALEAEG FGLPEEKGPD LYLIPLTEEA VAEAFYLAEA
360 370 380 390 400
LRPRLRAEYA LAPRKPAKGL EEALKRGAAF AGFLGEDELR AGEVTLKRLA
410 420
TGEQVRLSRE EVPGYLLQAL G
Length:421
Mass (Da):47,041
Last modified:March 29, 2005 - v2
Checksum:i9DEEE25F2C570A27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200L → E (PubMed:9115984).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70535.1.
RefSeqiWP_011172810.1. NC_006461.1.
YP_143978.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70535; BAD70535; BAD70535.
GeneIDi3168691.
KEGGittj:TTHA0712.
PATRICi23956401. VBITheThe93045_0706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70535.1.
RefSeqiWP_011172810.1. NC_006461.1.
YP_143978.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ADJX-ray2.70A/B/C/D1-421[»]
1ADYX-ray2.80A/B/C/D1-421[»]
ProteinModelPortaliP56194.
SMRiP56194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70535; BAD70535; BAD70535.
GeneIDi3168691.
KEGGittj:TTHA0712.
PATRICi23956401. VBITheThe93045_0706.

Phylogenomic databases

eggNOGiENOG4105CUT. Bacteria.
COG0124. LUCA.
HOGENOMiHOG000018072.
KOiK01892.
OMAiCGGGNFK.
PhylomeDBiP56194.

Enzyme and pathway databases

BRENDAi6.1.1.21. 2305.

Miscellaneous databases

EvolutionaryTraceiP56194.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYH_THET8
AccessioniPrimary (citable) accession number: P56194
Secondary accession number(s): Q5SKD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.