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P56192

- SYMC_HUMAN

UniProt

P56192 - SYMC_HUMAN

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Protein

Methionine--tRNA ligase, cytoplasmic

Gene
MARS
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. methionine-tRNA ligase activity Source: Reactome
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. gene expression Source: Reactome
  2. methionyl-tRNA aminoacylation Source: InterPro
  3. tRNA aminoacylation for protein translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

ReactomeiREACT_15306. Cytosolic tRNA aminoacylation.
SignaLinkiP56192.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:MARS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:6898. MARS.

Subcellular locationi

Cytoplasm Inferred UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. extracellular vesicular exosome Source: UniProt
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Infantile liver failure syndrome 2 (ILFS2) [MIM:615486]: A life-threatening disorder of hepatic function that manifests with liver failure in the first months of life. Clinical features include failure to thrive, hypotonia, intermittent lactic acidosis, aminoaciduria, hypothyroidism, interstitial lung disease, anemia, liver canalicular cholestasis, steatosis, and iron deposition.
Note: The disease is caused by mutations affecting the gene represented in this entry.1 Publication
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti370 – 3701F → L in ILFS2. 1 Publication
VAR_070872
Natural varianti523 – 5231I → T in ILFS2. 1 Publication
VAR_070873

Keywords - Diseasei

Disease mutation

Organism-specific databases

MIMi615486. phenotype.
Orphaneti370088. Acute infantile liver failure-multisystemic involvement syndrome.
PharmGKBiPA30642.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Methionine--tRNA ligase, cytoplasmicUniRule annotationPRO_0000139262Add
BLAST

Proteomic databases

MaxQBiP56192.
PaxDbiP56192.
PeptideAtlasiP56192.
PRIDEiP56192.

PTM databases

PhosphoSiteiP56192.

Expressioni

Gene expression databases

ArrayExpressiP56192.
BgeeiP56192.
CleanExiHS_MARS.
GenevestigatoriP56192.

Organism-specific databases

HPAiCAB017097.
HPA004125.

Interactioni

Subunit structurei

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

Protein-protein interaction databases

BioGridi110311. 39 interactions.
DIPiDIP-38164N.
IntActiP56192. 14 interactions.
MINTiMINT-5004366.
STRINGi9606.ENSP00000262027.

Structurei

Secondary structure

1
900
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54
Helixi12 – 187
Helixi19 – 224
Turni23 – 253
Beta strandi29 – 324
Beta strandi48 – 525
Helixi62 – 7211
Helixi79 – 9012
Helixi92 – 10413
Helixi111 – 1144
Turni115 – 1173
Helixi118 – 13013
Beta strandi131 – 14010
Helixi143 – 15614
Helixi159 – 1613
Helixi167 – 17711
Helixi180 – 19011
Helixi196 – 1983
Helixi199 – 2035
Helixi838 – 86124
Helixi866 – 88722
Beta strandi893 – 8953
Beta strandi897 – 8993

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DJVNMR-A835-900[»]
4BL7X-ray1.89A1-224[»]
ProteinModelPortaliP56192.
SMRiP56192. Positions 267-893.

Miscellaneous databases

EvolutionaryTraceiP56192.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 198125GST C-terminalAdd
BLAST
Domaini841 – 89757WHEP-TRSAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi273 – 28311"HIGH" regionUniRule annotationAdd
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Sequence similaritiesi

Contains 1 WHEP-TRS domain.

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200402.
HOVERGENiHBG036191.
InParanoidiP56192.
KOiK01874.
OMAiFIKADTY.
PhylomeDBiP56192.
TreeFamiTF300526.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR000738. WHEP-TRS.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56192-1 [UniParc]FASTAAdd to Basket

« Hide

MRLFVSDGVP GCLPVLAAAG RARGRAEVLI STVGPEDCVV PFLTRPKVPV    50
LQLDSGNYLF STSAICRYFF LLSGWEQDDL TNQWLEWEAT ELQPALSAAL 100
YYLVVQGKKG EDVLGSVRRA LTHIDHSLSR QNCPFLAGET ESLADIVLWG 150
ALYPLLQDPA YLPEELSALH SWFQTLSTQE PCQRAAETVL KQQGVLALRP 200
YLQKQPQPSP AEGRAVTNEP EEEELATLSE EEIAMAVTAW EKGLESLPPL 250
RPQQNPVLPV AGERNVLITS ALPYVNNVPH LGNIIGCVLS ADVFARYSRL 300
RQWNTLYLCG TDEYGTATET KALEEGLTPQ EICDKYHIIH ADIYRWFNIS 350
FDIFGRTTTP QQTKITQDIF QQLLKRGFVL QDTVEQLRCE HCARFLADRF 400
VEGVCPFCGY EEARGDQCDK CGKLINAVEL KKPQCKVCRS CPVVQSSQHL 450
FLDLPKLEKR LEEWLGRTLP GSDWTPNAQF ITRSWLRDGL KPRCITRDLK 500
WGTPVPLEGF EDKVFYVWFD ATIGYLSITA NYTDQWERWW KNPEQVDLYQ 550
FMAKDNVPFH SLVFPCSALG AEDNYTLVSH LIATEYLNYE DGKFSKSRGV 600
GVFGDMAQDT GIPADIWRFY LLYIRPEGQD SAFSWTDLLL KNNSELLNNL 650
GNFINRAGMF VSKFFGGYVP EMVLTPDDQR LLAHVTLELQ HYHQLLEKVR 700
IRDALRSILT ISRHGNQYIQ VNEPWKRIKG SEADRQRAGT VTGLAVNIAA 750
LLSVMLQPYM PTVSATIQAQ LQLPPPACSI LLTNFLCTLP AGHQIGTVSP 800
LFQKLENDQI ESLRQRFGGG QAKTSPKPAV VETVTTAKPQ QIQALMDEVT 850
KQGNIVRELK AQKADKNEVA AEVAKLLDLK KQLAVAEGKP PEAPKGKKKK 900
Length:900
Mass (Da):101,116
Last modified:April 16, 2002 - v2
Checksum:i3D687C77E17C5C96
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti370 – 3701F → L in ILFS2. 1 Publication
VAR_070872
Natural varianti523 – 5231I → T in ILFS2. 1 Publication
VAR_070873
Natural varianti683 – 6831A → D.
Corresponds to variant rs1054403 [ dbSNP | Ensembl ].
VAR_020459

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531L → V in CAA64381. 1 Publication
Sequence conflicti99 – 991A → P in CAA64381. 1 Publication
Sequence conflicti152 – 1521L → Q in CAA64381. 1 Publication
Sequence conflicti172 – 1721W → S in AAH15011. 1 Publication
Sequence conflicti250 – 2501L → P in BAD96487. 1 Publication
Sequence conflicti683 – 6831A → G in CAA64381. 1 Publication
Sequence conflicti683 – 6831A → G in CAA89153. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94754 mRNA. Translation: CAA64381.1.
Z49216 mRNA. Translation: CAA89153.1.
D84224 mRNA. Translation: BAA95668.1.
BT007338 mRNA. Translation: AAP36002.1.
AK222767 mRNA. Translation: BAD96487.1.
BC002384 mRNA. Translation: AAH02384.1.
BC006328 mRNA. Translation: AAH06328.1.
BC011639 mRNA. Translation: AAH11639.1.
BC011849 mRNA. Translation: AAH11849.1.
BC015011 mRNA. Translation: AAH15011.1.
CCDSiCCDS8942.1.
PIRiJC5224.
RefSeqiNP_004981.2. NM_004990.3.
UniGeneiHs.632707.

Genome annotation databases

EnsembliENST00000262027; ENSP00000262027; ENSG00000166986.
GeneIDi4141.
KEGGihsa:4141.
UCSCiuc001sog.3. human.

Polymorphism databases

DMDMi20178332.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94754 mRNA. Translation: CAA64381.1 .
Z49216 mRNA. Translation: CAA89153.1 .
D84224 mRNA. Translation: BAA95668.1 .
BT007338 mRNA. Translation: AAP36002.1 .
AK222767 mRNA. Translation: BAD96487.1 .
BC002384 mRNA. Translation: AAH02384.1 .
BC006328 mRNA. Translation: AAH06328.1 .
BC011639 mRNA. Translation: AAH11639.1 .
BC011849 mRNA. Translation: AAH11849.1 .
BC015011 mRNA. Translation: AAH15011.1 .
CCDSi CCDS8942.1.
PIRi JC5224.
RefSeqi NP_004981.2. NM_004990.3.
UniGenei Hs.632707.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DJV NMR - A 835-900 [» ]
4BL7 X-ray 1.89 A 1-224 [» ]
ProteinModelPortali P56192.
SMRi P56192. Positions 267-893.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 110311. 39 interactions.
DIPi DIP-38164N.
IntActi P56192. 14 interactions.
MINTi MINT-5004366.
STRINGi 9606.ENSP00000262027.

Chemistry

BindingDBi P56192.
ChEMBLi CHEMBL2870.
DrugBanki DB00134. L-Methionine.

PTM databases

PhosphoSitei P56192.

Polymorphism databases

DMDMi 20178332.

Proteomic databases

MaxQBi P56192.
PaxDbi P56192.
PeptideAtlasi P56192.
PRIDEi P56192.

Protocols and materials databases

DNASUi 4141.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000262027 ; ENSP00000262027 ; ENSG00000166986 .
GeneIDi 4141.
KEGGi hsa:4141.
UCSCi uc001sog.3. human.

Organism-specific databases

CTDi 4141.
GeneCardsi GC12P057881.
HGNCi HGNC:6898. MARS.
HPAi CAB017097.
HPA004125.
MIMi 156560. gene.
615486. phenotype.
neXtProti NX_P56192.
Orphaneti 370088. Acute infantile liver failure-multisystemic involvement syndrome.
PharmGKBi PA30642.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0143.
HOGENOMi HOG000200402.
HOVERGENi HBG036191.
InParanoidi P56192.
KOi K01874.
OMAi FIKADTY.
PhylomeDBi P56192.
TreeFami TF300526.

Enzyme and pathway databases

Reactomei REACT_15306. Cytosolic tRNA aminoacylation.
SignaLinki P56192.

Miscellaneous databases

ChiTaRSi MARS. human.
EvolutionaryTracei P56192.
GeneWikii MARS_(gene).
GenomeRNAii 4141.
NextBioi 16264.
PROi P56192.
SOURCEi Search...

Gene expression databases

ArrayExpressi P56192.
Bgeei P56192.
CleanExi HS_MARS.
Genevestigatori P56192.

Family and domain databases

Gene3Di 1.10.287.10. 1 hit.
1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00098. Met_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR000738. WHEP-TRS.
[Graphical view ]
Pfami PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view ]
PRINTSi PR01041. TRNASYNTHMET.
SMARTi SM00991. WHEP-TRS. 1 hit.
[Graphical view ]
SUPFAMi SSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsi TIGR00398. metG. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a human cDNA encoding a protein with high homology to yeast methionyl-tRNA synthetase."
    Lage H., Dietel M.
    Gene 178:187-189(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Gastric carcinoma.
  2. "Cloning and sequence determination of a human cytoplasmic methionyl-tRNA synthetase gene."
    Motegi H., Noda T., Shiba K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Colon and Muscle.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 835-900.
  13. "Rare recessive loss-of-function methionyl-tRNA synthetase mutations presenting as a multi-organ phenotype."
    van Meel E., Wegner D.J., Cliften P., Willing M.C., White F.V., Kornfeld S., Cole F.S.
    BMC Med. Genet. 14:106-106(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS ILFS2 LEU-370 AND THR-523.

Entry informationi

Entry nameiSYMC_HUMAN
AccessioniPrimary (citable) accession number: P56192
Secondary accession number(s): Q14895
, Q53H14, Q96A15, Q96BZ0, Q9NSE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 16, 2002
Last modified: September 3, 2014
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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