Methionyl-tRNA synthetase, cytoplasmic

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Reviewed, UniProtKB/Swiss-Prot P56192 (SYMC_HUMAN)

Last modified July 7, 2009. Version 94. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:

MARS

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subunit structure	Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 GST C-terminal domain. Contains 1 WHEP-TRS domain.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
BRF2	Q9HAW0	1	EBI-355848,EBI-1055224
GIPC1	O14908	1	EBI-355848,EBI-373132
ILK	Q13418	1	EBI-355848,EBI-747644
MCC	P23508	1	EBI-355848,EBI-307531
PRKAB1	Q9Y478	1	EBI-355848,EBI-719769

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 900

900

Methionyl-tRNA synthetase, cytoplasmic

PRO_0000139262

Regions

Domain

74 – 198

125

GST C-terminal

Domain

841 – 897

WHEP-TRS

Motif

273 – 283

"HIGH" region

Motif

593 – 597

"KMSKS" region

Sites

Binding site

596

ATP By similarity

Natural variations

Natural variant

683

A → D: dbSNP rs1054403.

VAR_020459

Experimental info

Sequence conflict

L → V in CAA64381. Ref.1

Sequence conflict

A → P in CAA64381. Ref.1

Sequence conflict

152

L → Q in CAA64381. Ref.1

Sequence conflict

172

W → S in AAH15011. Ref.5

Sequence conflict

250

L → P in BAD96487. Ref.4

Sequence conflict

683

A → G in CAA64381. Ref.1

Sequence conflict

683

A → G in CAA89153. Ref.1

Secondary structure

900

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P56192-1 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: 3D687C77E17C5C96
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FASTA

900

101,116

        10         20         30         40         50         60 
MRLFVSDGVP GCLPVLAAAG RARGRAEVLI STVGPEDCVV PFLTRPKVPV LQLDSGNYLF 

        70         80         90        100        110        120 
STSAICRYFF LLSGWEQDDL TNQWLEWEAT ELQPALSAAL YYLVVQGKKG EDVLGSVRRA 

       130        140        150        160        170        180 
LTHIDHSLSR QNCPFLAGET ESLADIVLWG ALYPLLQDPA YLPEELSALH SWFQTLSTQE 

       190        200        210        220        230        240 
PCQRAAETVL KQQGVLALRP YLQKQPQPSP AEGRAVTNEP EEEELATLSE EEIAMAVTAW 

       250        260        270        280        290        300 
EKGLESLPPL RPQQNPVLPV AGERNVLITS ALPYVNNVPH LGNIIGCVLS ADVFARYSRL 

       310        320        330        340        350        360 
RQWNTLYLCG TDEYGTATET KALEEGLTPQ EICDKYHIIH ADIYRWFNIS FDIFGRTTTP 

       370        380        390        400        410        420 
QQTKITQDIF QQLLKRGFVL QDTVEQLRCE HCARFLADRF VEGVCPFCGY EEARGDQCDK 

       430        440        450        460        470        480 
CGKLINAVEL KKPQCKVCRS CPVVQSSQHL FLDLPKLEKR LEEWLGRTLP GSDWTPNAQF 

       490        500        510        520        530        540 
ITRSWLRDGL KPRCITRDLK WGTPVPLEGF EDKVFYVWFD ATIGYLSITA NYTDQWERWW 

       550        560        570        580        590        600 
KNPEQVDLYQ FMAKDNVPFH SLVFPCSALG AEDNYTLVSH LIATEYLNYE DGKFSKSRGV 

       610        620        630        640        650        660 
GVFGDMAQDT GIPADIWRFY LLYIRPEGQD SAFSWTDLLL KNNSELLNNL GNFINRAGMF 

       670        680        690        700        710        720 
VSKFFGGYVP EMVLTPDDQR LLAHVTLELQ HYHQLLEKVR IRDALRSILT ISRHGNQYIQ 

       730        740        750        760        770        780 
VNEPWKRIKG SEADRQRAGT VTGLAVNIAA LLSVMLQPYM PTVSATIQAQ LQLPPPACSI 

       790        800        810        820        830        840 
LLTNFLCTLP AGHQIGTVSP LFQKLENDQI ESLRQRFGGG QAKTSPKPAV VETVTTAKPQ 

       850        860        870        880        890        900 
QIQALMDEVT KQGNIVRELK AQKADKNEVA AEVAKLLDLK KQLAVAEGKP PEAPKGKKKK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human cDNA encoding a protein with high homology to yeast methionyl-tRNA synthetase." Lage H., Dietel M. Gene 178:187-189(1996) [PubMed: 8921912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gastric carcinoma.
[2]	"Cloning and sequence determination of a human cytoplasmic methionyl-tRNA synthetase gene." Motegi H., Noda T., Shiba K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Colon and Muscle.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 835-900.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X94754 mRNA. Translation: CAA64381.1.
Z49216 mRNA. Translation: CAA89153.1.
D84224 mRNA. Translation: BAA95668.1.
BT007338 mRNA. Translation: AAP36002.1.
AK222767 mRNA. Translation: BAD96487.1.
BC002384 mRNA. Translation: AAH02384.1.
BC006328 mRNA. Translation: AAH06328.1.
BC011639 mRNA. Translation: AAH11639.1.
BC011849 mRNA. Translation: AAH11849.1.
BC015011 mRNA. Translation: AAH15011.1.

IPI

IPI00008240.

PIR

JC5224.

RefSeq

NP_004981.2.

UniGene

Hs.632707

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DJV	NMR	-	A	835-900	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P56192. 20 interactions.

PTM databases

PhosphoSite

P56192.

Proteomic databases

PeptideAtlas

P56192.

PRIDE

P56192.

Genome annotation databases

Ensembl

ENSG00000166986. Homo sapiens. [Contig view]

GeneID

4141.

KEGG

hsa:4141.

NMPDR

fig|9606.3.peg.7802.

UCSC

uc001sog.1. human.

Organism-specific databases

GeneCards

GC12P056167.

H-InvDB

HIX0022196.

HGNC

HGNC:6898. MARS.

HPA

CAB017097.
HPA004125.

MIM

156560. gene.

PharmGKB

PA30642.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P56192.

OMA

P56192. CEAVPES.

Enzyme and pathway databases

BRENDA

6.1.1.10. 247.

Reactome

REACT_71. Gene Expression.

Gene expression databases

Bgee

P56192.

CleanEx

HS_MARS.

GermOnline

ENSG00000166986. Homo sapiens.

Family and domain databases

InterPro

IPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR002304. Met-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA_bd.
IPR014758. tRNA-synt_met_N.
IPR000738. WHEP-TRS.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]

PRINTS

PR01041. TRNASYNTHMET.

TIGRFAMs

TIGR00398. metG. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00134. L-Methionine.

NextBio

16264.

SOURCE

Search...

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Entry information

Entry name

SYMC_HUMAN

Accession

Primary (citable) accession number: P56192
Secondary accession number(s): Q14895

Q9NSE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 16, 2002
Last modified:	July 7, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents