P56185 (RNJ_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease J
Short name=RNase J
EC=3.1.-.-

Gene names

Ordered Locus Names:

HP_1430

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Divalent metal cations By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Ribonuclease J		PRO_0000215269
Sites
	Metal binding	206	1	Divalent metal cation 1 By similarity
	Metal binding	208	1	Divalent metal cation 1 By similarity
	Metal binding	210	1	Divalent metal cation 2 By similarity
	Metal binding	211	1	Divalent metal cation 2 By similarity
	Metal binding	275	1	Divalent metal cation 1 By similarity
	Metal binding	297	1	Divalent metal cation 1 By similarity
	Metal binding	297	1	Divalent metal cation 2 By similarity
	Metal binding	533	1	Divalent metal cation 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56185 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 77791982943F5B80	FASTA	689	77,386
	10 20 30 40 50 60 MTDNNQNNEN HENSSENSKA DEMRAGAFER FTNRKKRFRE NAQKNAEYSN HEASSHHKKE 70 80 90 100 110 120 HRPNKKPNNH HKQKHAKTRN YAQEELDSNK VEGVTEILHV NERGTLGFHK ELKKGVEANN 130 140 150 160 170 180 KIQVEHLNPH YKMNLNSKAS VKITPLGGLG EIGGNMMVIE TPKSAIVIDA GMSFPKEGLF 190 200 210 220 230 240 GVDILIPDFS YLHQIKDKIA GIIITHAHED HIGATPYLFK ELQFPLYGTP LSLGLIGSKF 250 260 270 280 290 300 DEHGLKKYRS YFKIVEKRCP ISVGEFIIEW IHITHSIIDS SALAIQTKAG TIIHTGDFKI 310 320 330 340 350 360 DHTPVDNLPT DLYRLAHYGE KGVMLLLSDS TNSHKSGTTP SESTIAPAFD TLFKEAQGRV 370 380 390 400 410 420 IMSTFSSNIH RVYQAIQYGI KYNRKIAVIG RSMEKNLDIA RELGYIHLPY QSFIEANEVA 430 440 450 460 470 480 KYPDNEILIV TTGSQGETMS ALYRMATDEH RHISIKPNDL VIISAKAIPG NEASVSAVLN 490 500 510 520 530 540 FLIKKEAKVA YQEFDNIHVS GHAAQEEQKL MLRLIKPKFF LPVHGEYNHV ARHKQTAISC 550 560 570 580 590 600 GVPEKNIYLM EDGDQVEVGP AFIKKVGTIK SGKSYVDNQS NLSIDTSIVQ QREEVASAGV 610 620 630 640 650 660 FVATIFVNKN KQALLESSQF SSLGLVGFKD EKPLIKEIQG GLEVLLKSSN AEILNNPKKL 670 680 EDHTRNFIRK ALFKKFRKYP AIICHAHSF

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08469.1.
PIR	F64698.
RefSeq	NP_208221.1. NC_000915.1. YP_006935351.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56185.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3218N.
MINT	MINT-177855.
STRING	85962.HP1430.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08469; AAD08469; HP_1430.
GeneID	13870640. 899865.
KEGG	heo:C694_07395. hpy:HP1430.
PATRIC	20594259. VBIHelPyl33062_1499.
Phylogenomic databases
eggNOG	COG0595.
KO	K12574.
OMA	NIHVSGH.
ProtClustDB	CLSK872552.
Family and domain databases
InterPro	IPR001279. Beta-lactamas-like. IPR011108. RMMBL. IPR004613. RNase_J. IPR001587. RNase_J_CS. [Graphical view]
PANTHER	PTHR11203:SF22. PTHR11203:SF22. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. PF07521. RMMBL. 1 hit. [Graphical view]
PIRSF	PIRSF004803. RnjA. 1 hit.
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR00649. MG423. 1 hit.
PROSITE	PS01292. UPF0036. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNJ_HELPY

Accession

Primary (citable) accession number: P56185

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program