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P56185

- RNJ_HELPY

UniProt

P56185 - RNJ_HELPY

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Protein

Ribonuclease J

Gene

rnj

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

An RNase that has 5'-3' exonuclease and possibly endoonuclease activity. Involved in maturation of mRNA but apparently not rRNA (By similarity).By similarity

Cofactori

Zn2+UniRule annotationNote: Binds up to 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi206 – 2061Zinc 1; catalyticUniRule annotation
Metal bindingi208 – 2081Zinc 1; catalyticUniRule annotation
Metal bindingi210 – 2101Zinc 2; catalyticUniRule annotation
Metal bindingi211 – 2111Zinc 2; catalyticUniRule annotation
Metal bindingi275 – 2751Zinc 1; catalyticUniRule annotation
Metal bindingi297 – 2971Zinc 1; catalyticUniRule annotation
Metal bindingi297 – 2971Zinc 2; catalyticUniRule annotation
Metal bindingi524 – 5241Zinc 2; catalyticUniRule annotation

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. exonuclease activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. rRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP1430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease JUniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
RNase JUniRule annotation
Gene namesi
Name:rnjUniRule annotation
Ordered Locus Names:HP_1430
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 689689Ribonuclease JPRO_0000215269Add
BLAST

Proteomic databases

PRIDEiP56185.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with RNA helicase RhpA.1 PublicationCurated

Protein-protein interaction databases

DIPiDIP-3218N.
IntActiP56185. 14 interactions.
MINTiMINT-177855.
STRINGi85962.HP1430.

Structurei

3D structure databases

ProteinModelPortaliP56185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni498 – 5025Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0595.
KOiK12574.
OMAiNIARRKM.
OrthoDBiEOG6P5ZDC.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01491. RNase_J_bact.
InterProiIPR001279. Beta-lactamas-like.
IPR011108. RMMBL.
IPR004613. RNase_J.
IPR001587. RNase_J_CS.
[Graphical view]
PANTHERiPTHR11203:SF22. PTHR11203:SF22. 1 hit.
PfamiPF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view]
PIRSFiPIRSF004803. RnjA. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR00649. MG423. 1 hit.
PROSITEiPS01292. UPF0036. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56185-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDNNQNNEN HENSSENSKA DEMRAGAFER FTNRKKRFRE NAQKNAEYSN
60 70 80 90 100
HEASSHHKKE HRPNKKPNNH HKQKHAKTRN YAQEELDSNK VEGVTEILHV
110 120 130 140 150
NERGTLGFHK ELKKGVEANN KIQVEHLNPH YKMNLNSKAS VKITPLGGLG
160 170 180 190 200
EIGGNMMVIE TPKSAIVIDA GMSFPKEGLF GVDILIPDFS YLHQIKDKIA
210 220 230 240 250
GIIITHAHED HIGATPYLFK ELQFPLYGTP LSLGLIGSKF DEHGLKKYRS
260 270 280 290 300
YFKIVEKRCP ISVGEFIIEW IHITHSIIDS SALAIQTKAG TIIHTGDFKI
310 320 330 340 350
DHTPVDNLPT DLYRLAHYGE KGVMLLLSDS TNSHKSGTTP SESTIAPAFD
360 370 380 390 400
TLFKEAQGRV IMSTFSSNIH RVYQAIQYGI KYNRKIAVIG RSMEKNLDIA
410 420 430 440 450
RELGYIHLPY QSFIEANEVA KYPDNEILIV TTGSQGETMS ALYRMATDEH
460 470 480 490 500
RHISIKPNDL VIISAKAIPG NEASVSAVLN FLIKKEAKVA YQEFDNIHVS
510 520 530 540 550
GHAAQEEQKL MLRLIKPKFF LPVHGEYNHV ARHKQTAISC GVPEKNIYLM
560 570 580 590 600
EDGDQVEVGP AFIKKVGTIK SGKSYVDNQS NLSIDTSIVQ QREEVASAGV
610 620 630 640 650
FVATIFVNKN KQALLESSQF SSLGLVGFKD EKPLIKEIQG GLEVLLKSSN
660 670 680
AEILNNPKKL EDHTRNFIRK ALFKKFRKYP AIICHAHSF
Length:689
Mass (Da):77,386
Last modified:November 1, 1997 - v1
Checksum:i77791982943F5B80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08469.1.
PIRiF64698.
RefSeqiNP_208221.1. NC_000915.1.
YP_006935351.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08469; AAD08469; HP_1430.
GeneIDi13870640.
899865.
KEGGiheo:C694_07395.
hpy:HP1430.
PATRICi20594259. VBIHelPyl33062_1499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08469.1 .
PIRi F64698.
RefSeqi NP_208221.1. NC_000915.1.
YP_006935351.1. NC_018939.1.

3D structure databases

ProteinModelPortali P56185.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3218N.
IntActi P56185. 14 interactions.
MINTi MINT-177855.
STRINGi 85962.HP1430.

Proteomic databases

PRIDEi P56185.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08469 ; AAD08469 ; HP_1430 .
GeneIDi 13870640.
899865.
KEGGi heo:C694_07395.
hpy:HP1430.
PATRICi 20594259. VBIHelPyl33062_1499.

Phylogenomic databases

eggNOGi COG0595.
KOi K12574.
OMAi NIARRKM.
OrthoDBi EOG6P5ZDC.

Enzyme and pathway databases

BioCyci HPY:HP1430-MONOMER.

Family and domain databases

Gene3Di 3.60.15.10. 1 hit.
HAMAPi MF_01491. RNase_J_bact.
InterProi IPR001279. Beta-lactamas-like.
IPR011108. RMMBL.
IPR004613. RNase_J.
IPR001587. RNase_J_CS.
[Graphical view ]
PANTHERi PTHR11203:SF22. PTHR11203:SF22. 1 hit.
Pfami PF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view ]
PIRSFi PIRSF004803. RnjA. 1 hit.
SMARTi SM00849. Lactamase_B. 1 hit.
[Graphical view ]
SUPFAMi SSF56281. SSF56281. 1 hit.
TIGRFAMsi TIGR00649. MG423. 1 hit.
PROSITEi PS01292. UPF0036. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "A minimal bacterial RNase J-based degradosome is associated with translating ribosomes."
    Redko Y., Aubert S., Stachowicz A., Lenormand P., Namane A., Darfeuille F., Thibonnier M., De Reuse H.
    Nucleic Acids Res. 41:288-301(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RHPA, SUBUNIT.
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiRNJ_HELPY
AccessioniPrimary (citable) accession number: P56185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3