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P56185

- RNJ_HELPY

UniProt

P56185 - RNJ_HELPY

Protein

Ribonuclease J

Gene

rnj

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    An RNase that has 5'-3' exonuclease and possibly endoonuclease activity. Involved in maturation of mRNA but apparently not rRNA By similarity.By similarity

    Cofactori

    Binds up to 2 Zn2+ ions per subunit. It is not clear if Zn2+ or Mg2+ is physiologically important.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi206 – 2061Zinc 1; catalyticUniRule annotation
    Metal bindingi208 – 2081Zinc 1; catalyticUniRule annotation
    Metal bindingi210 – 2101Zinc 2; catalyticUniRule annotation
    Metal bindingi211 – 2111Zinc 2; catalyticUniRule annotation
    Metal bindingi275 – 2751Zinc 1; catalyticUniRule annotation
    Metal bindingi297 – 2971Zinc 1; catalyticUniRule annotation
    Metal bindingi297 – 2971Zinc 2; catalyticUniRule annotation
    Metal bindingi524 – 5241Zinc 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. exonuclease activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. rRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciHPY:HP1430-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease JUniRule annotation (EC:3.1.-.-UniRule annotation)
    Short name:
    RNase JUniRule annotation
    Gene namesi
    Name:rnjUniRule annotation
    Ordered Locus Names:HP_1430
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 689689Ribonuclease JPRO_0000215269Add
    BLAST

    Proteomic databases

    PRIDEiP56185.

    Interactioni

    Subunit structurei

    Homodimer Probable. Interacts with RNA helicase RhpA.1 PublicationCurated

    Protein-protein interaction databases

    DIPiDIP-3218N.
    IntActiP56185. 14 interactions.
    MINTiMINT-177855.
    STRINGi85962.HP1430.

    Structurei

    3D structure databases

    ProteinModelPortaliP56185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni498 – 5025Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0595.
    KOiK12574.
    OMAiNIARRKM.
    OrthoDBiEOG6P5ZDC.

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    HAMAPiMF_01491. RNase_J_bact.
    InterProiIPR001279. Beta-lactamas-like.
    IPR011108. RMMBL.
    IPR004613. RNase_J.
    IPR001587. RNase_J_CS.
    [Graphical view]
    PANTHERiPTHR11203:SF22. PTHR11203:SF22. 1 hit.
    PfamiPF00753. Lactamase_B. 1 hit.
    PF07521. RMMBL. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004803. RnjA. 1 hit.
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.
    TIGRFAMsiTIGR00649. MG423. 1 hit.
    PROSITEiPS01292. UPF0036. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56185-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDNNQNNEN HENSSENSKA DEMRAGAFER FTNRKKRFRE NAQKNAEYSN    50
    HEASSHHKKE HRPNKKPNNH HKQKHAKTRN YAQEELDSNK VEGVTEILHV 100
    NERGTLGFHK ELKKGVEANN KIQVEHLNPH YKMNLNSKAS VKITPLGGLG 150
    EIGGNMMVIE TPKSAIVIDA GMSFPKEGLF GVDILIPDFS YLHQIKDKIA 200
    GIIITHAHED HIGATPYLFK ELQFPLYGTP LSLGLIGSKF DEHGLKKYRS 250
    YFKIVEKRCP ISVGEFIIEW IHITHSIIDS SALAIQTKAG TIIHTGDFKI 300
    DHTPVDNLPT DLYRLAHYGE KGVMLLLSDS TNSHKSGTTP SESTIAPAFD 350
    TLFKEAQGRV IMSTFSSNIH RVYQAIQYGI KYNRKIAVIG RSMEKNLDIA 400
    RELGYIHLPY QSFIEANEVA KYPDNEILIV TTGSQGETMS ALYRMATDEH 450
    RHISIKPNDL VIISAKAIPG NEASVSAVLN FLIKKEAKVA YQEFDNIHVS 500
    GHAAQEEQKL MLRLIKPKFF LPVHGEYNHV ARHKQTAISC GVPEKNIYLM 550
    EDGDQVEVGP AFIKKVGTIK SGKSYVDNQS NLSIDTSIVQ QREEVASAGV 600
    FVATIFVNKN KQALLESSQF SSLGLVGFKD EKPLIKEIQG GLEVLLKSSN 650
    AEILNNPKKL EDHTRNFIRK ALFKKFRKYP AIICHAHSF 689
    Length:689
    Mass (Da):77,386
    Last modified:November 1, 1997 - v1
    Checksum:i77791982943F5B80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08469.1.
    PIRiF64698.
    RefSeqiNP_208221.1. NC_000915.1.
    YP_006935351.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD08469; AAD08469; HP_1430.
    GeneIDi13870640.
    899865.
    KEGGiheo:C694_07395.
    hpy:HP1430.
    PATRICi20594259. VBIHelPyl33062_1499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08469.1 .
    PIRi F64698.
    RefSeqi NP_208221.1. NC_000915.1.
    YP_006935351.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali P56185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3218N.
    IntActi P56185. 14 interactions.
    MINTi MINT-177855.
    STRINGi 85962.HP1430.

    Proteomic databases

    PRIDEi P56185.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD08469 ; AAD08469 ; HP_1430 .
    GeneIDi 13870640.
    899865.
    KEGGi heo:C694_07395.
    hpy:HP1430.
    PATRICi 20594259. VBIHelPyl33062_1499.

    Phylogenomic databases

    eggNOGi COG0595.
    KOi K12574.
    OMAi NIARRKM.
    OrthoDBi EOG6P5ZDC.

    Enzyme and pathway databases

    BioCyci HPY:HP1430-MONOMER.

    Family and domain databases

    Gene3Di 3.60.15.10. 1 hit.
    HAMAPi MF_01491. RNase_J_bact.
    InterProi IPR001279. Beta-lactamas-like.
    IPR011108. RMMBL.
    IPR004613. RNase_J.
    IPR001587. RNase_J_CS.
    [Graphical view ]
    PANTHERi PTHR11203:SF22. PTHR11203:SF22. 1 hit.
    Pfami PF00753. Lactamase_B. 1 hit.
    PF07521. RMMBL. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004803. RnjA. 1 hit.
    SMARTi SM00849. Lactamase_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56281. SSF56281. 1 hit.
    TIGRFAMsi TIGR00649. MG423. 1 hit.
    PROSITEi PS01292. UPF0036. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    2. "A minimal bacterial RNase J-based degradosome is associated with translating ribosomes."
      Redko Y., Aubert S., Stachowicz A., Lenormand P., Namane A., Darfeuille F., Thibonnier M., De Reuse H.
      Nucleic Acids Res. 41:288-301(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RHPA, SUBUNIT.
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiRNJ_HELPY
    AccessioniPrimary (citable) accession number: P56185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3