P56162 (PYRE_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	HP_1257

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110701
Regions
	Region	113 – 121	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	117	1	Orotate By similarity
	Binding site	145	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56162 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9FD8A1DE81787837	FASTA	201	21,903
	10 20 30 40 50 60 MDIKACYQNA KALLEGHFLL SSGFHSNYYL QSAKVLEDPK LAEQLALELA KQIQEAHLNI 70 80 90 100 110 120 ECVCSPAIGG ILAGYELARA LGVRFIFTER VDNTMALRRG FEVKKNEKIL VCEDIITTGK 130 140 150 160 170 180 SAMECAKVLE EKGAQIVAFG ALANRGICKR AHSHLKAQEG ACLPSHLPLF ALEDFVFDMH 190 200 KPSSCPLCAT SVAIKPGSRG N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Crystallization and preliminary X-ray crystallographic analysis of orotate phosphoribosyltransferase from Helicobacter pylori." Kim M.-K., Song H.-E., Kim Y.S., Rho S.-H., Im Y.J., Lee J.H., Kang G.B., Eom S.H. Mol. Cells 15:361-363(2003) [PubMed: 12872993] [Abstract] Cited for: CRYSTALLIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08303.1.
PIR	A64677.
RefSeq	NP_208049.1. NC_000915.1.
3D structure databases
ProteinModelPortal	P56162.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	900320.
GenomeReviews	Gene locus HP_1257 in contig AE000511_GR.
KEGG	hpy:HP1257.
NMPDR	fig|85962.1.peg.1245.
PATRIC	20593891. VBIHelPyl33062_1316.
TIGR	HP_1257.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_HELPY

Accession

Primary (citable) accession number: P56162

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program