Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent Clp protease proteolytic subunit

Gene

clpP

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.UniRule annotation

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991NucleophileUniRule annotation
Active sitei124 – 1241UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciHPY:HP0794-MONOMER.

Protein family/group databases

MEROPSiS14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunitUniRule annotation (EC:3.4.21.92UniRule annotation)
Alternative name(s):
Endopeptidase ClpUniRule annotation
Gene namesi
Name:clpPUniRule annotation
Ordered Locus Names:HP_0794
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196ATP-dependent Clp protease proteolytic subunitPRO_0000179568Add
BLAST

Proteomic databases

PaxDbiP56156.
PRIDEiP56156.

Interactioni

Subunit structurei

Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3357N.
IntActiP56156. 1 interaction.
MINTiMINT-179817.
STRINGi85962.HP0794.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 266Combined sources
Turni27 – 293Combined sources
Beta strandi30 – 356Combined sources
Helixi39 – 5517Combined sources
Beta strandi57 – 593Combined sources
Beta strandi61 – 677Combined sources
Helixi72 – 8413Combined sources
Beta strandi85 – 873Combined sources
Beta strandi89 – 9810Combined sources
Helixi100 – 1056Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi120 – 1234Combined sources
Beta strandi127 – 1337Combined sources
Helixi134 – 15926Combined sources
Helixi163 – 1697Combined sources
Beta strandi174 – 1774Combined sources
Helixi178 – 1836Combined sources
Beta strandi188 – 1903Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZL0X-ray2.60A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
2ZL2X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
2ZL3X-ray2.81A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
2ZL4X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
ProteinModelPortaliP56156.
SMRiP56156. Positions 20-193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56156.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCQ. Bacteria.
COG0740. LUCA.
KOiK01358.
OMAiARMNELM.
OrthoDBiEOG6Z3KQ0.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00493. clpP. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMGYIPYVIE NTDRGERSYD IYSRLLKDRI VLLSGEINDS VASSIVAQLL
60 70 80 90 100
FLEAEDPEKD IGLYINSPGG VITSGLSIYD TMNFIRPDVS TICIGQAASM
110 120 130 140 150
GAFLLSCGAK GKRFSLPHSR IMIHQPLGGA QGQASDIEII SNEILRLKGL
160 170 180 190
MNSILAQNSG QSLEQIAKDT DRDFYMSAKE AKEYGLIDKV LQKNVK
Length:196
Mass (Da):21,526
Last modified:November 1, 1997 - v1
Checksum:i4E19BE5FE44656B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07842.1.
PIRiB64619.
RefSeqiNP_207587.1. NC_000915.1.
WP_001853304.1. NC_000915.1.

Genome annotation databases

EnsemblBacteriaiAAD07842; AAD07842; HP_0794.
GeneIDi898920.
KEGGihpy:HP0794.
PATRICi20592875. VBIHelPyl33062_0826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07842.1.
PIRiB64619.
RefSeqiNP_207587.1. NC_000915.1.
WP_001853304.1. NC_000915.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZL0X-ray2.60A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
2ZL2X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
2ZL3X-ray2.81A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
2ZL4X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N1-196[»]
ProteinModelPortaliP56156.
SMRiP56156. Positions 20-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3357N.
IntActiP56156. 1 interaction.
MINTiMINT-179817.
STRINGi85962.HP0794.

Protein family/group databases

MEROPSiS14.001.

Proteomic databases

PaxDbiP56156.
PRIDEiP56156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07842; AAD07842; HP_0794.
GeneIDi898920.
KEGGihpy:HP0794.
PATRICi20592875. VBIHelPyl33062_0826.

Phylogenomic databases

eggNOGiENOG4105CCQ. Bacteria.
COG0740. LUCA.
KOiK01358.
OMAiARMNELM.
OrthoDBiEOG6Z3KQ0.

Enzyme and pathway databases

BioCyciHPY:HP0794-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP56156.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00493. clpP. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiCLPP_HELPY
AccessioniPrimary (citable) accession number: P56156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 16, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.