Reviewed, UniProtKB/Swiss-Prot P56149 (ASPA_HELPY)

Names and origin

Protein names

Recommended name:
    Aspartate ammonia-lyase
      Short name=Aspartase
    EC=4.3.1.1

Gene names

Name:	aspA
Ordered Locus Names:	HP_0649

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate = fumarate + NH3.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	aspartate ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Aspartate ammonia-lyase		PRO_0000161342
Regions
	Region	138 – 140	3	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56149-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 60111E869302836A	FASTA	468	51,981
	10 20 30 40 50 60 MRIEHDFIGQ MEISDEVYYG IQTLRASENF FITNDKLCSY PVFIKSFAQV KKAATLANVQ 70 80 90 100 110 120 LGLIDEKLKI AICHACDLLI DGKYHDQFIV DMIQGGAGTS TNMNMNEVIA NLALEYMGHQ 130 140 150 160 170 180 KGEYQFCHPN DHVNRSQSTN DAYPSALKIA IYERLSNLVA PMKALRDAFA QKAKEFAHVI 190 200 210 220 230 240 KMGRTQLQDA VPMTLGQEFE TYALMVDRDI EQVLDARNWV RELNLGGTAI GTGINSHPDY 250 260 270 280 290 300 RSLIEKKIQE VTGRPFVMAN NLIEATQSTG AYVQVSGVLK RIAVKLSKVC NDLRLLSSGP 310 320 330 340 350 360 RAGLNEINLP KMQPGSSIMP GKVNPVIPEV VNQVCFAVIG NDLSVALAAE GGQLQLNVFE 370 380 390 400 410 420 PVIAYKLFHS FVILGRAIET LTTKCVEGIT ANEKICHDYV FNSIGIVTAL NPHIGYEKSA 430 440 450 460 MIAKEALKSD RSIYDIALEK KILTKEQLDD IFKPENMLSP HAFKKHKD

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07709.1.
PIR	A64601.
RefSeq	NP_207443.1.
3D structure databases
SMR	P56149. Positions 1-453.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3567N.
Genome annotation databases
GeneID	900208.
GenomeReviews	Gene locus HP_0649 in contig AE000511_GR.
KEGG	hpy:HP0649.
NMPDR	fig|85962.1.peg.641.
TIGR	HP_0649.
Phylogenomic databases
HOGENOM	HBG284369.
OMA	FPITGYR.
Enzyme and pathway databases
BRENDA	4.3.1.1. 1131.
Family and domain databases
InterPro	IPR004708. ApsA. IPR003031. D_crystallin. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00839. aspA. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPA_HELPY

Accession

Primary (citable) accession number: P56149

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)