Phenylalanine--tRNA ligase beta chain - Helicobacter pylori (strain ATCC 700392 / 26695)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	HP_0402

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00283
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 764

764

Phenylalanine--tRNA ligase beta chain HAMAP MF_00283

PRO_0000126893

Regions

Domain

38 – 148

111

tRNA-binding

Domain

375 – 455

81

B5

Domain

673 – 763

91

FDX-ACB

Sites

Metal binding

433

1

Magnesium By similarity

Metal binding

439

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

442

1

Magnesium By similarity

Metal binding

443

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P56145 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: CE182690F7B30C68
Show »

FASTA

764

85,136

        10         20         30         40         50         60 
MKLSINDLNV FVNTPKDIAK LCEDLSRLGL EVESCIPCIA PKNVVVGKIL EKAPHKNAEK 

        70         80         90        100        110        120 
LSVCQVDVGK EVLQIVCGAK NVAPNQFVPV ALNGALIGST TIAKTELRGV ESHGMICSSI 

       130        140        150        160        170        180 
ELGFPKINDG ILELDESVGE LVLGKELNEY APFNTHVLEI SLTPNRGDCL SVLGIAREIS 

       190        200        210        220        230        240 
AFYHTPLKPI KALNFTPKSG LITLSAGENI ESHLAYYLIC NHSLKTPLNI KLSLAHNNAL 

       250        260        270        280        290        300 
SENDLNNFIE FSTHFSGVIM NAYSLNTTPM DLSVKNDENN LESVYINHQK RSTIAIKHQV 

       310        320        330        340        350        360 
QKDLSECLLL EASYTDPISL SLKLHALKDK TLQKDNALIY RSARGSNPNL SDGLNFLSAH 

       370        380        390        400        410        420 
LKATILESKQ TEHSLKDRTL TFQLEDITEI LGLAVEKEKI QGILKNLGFK VSVKEPNSKP 

       430        440        450        460        470        480 
QILEVIAPNF RHDIKTIQDI AEEILRFVGI DNLVSKPLHC VSSKNSNPNY DTHRFFENLK 

       490        500        510        520        530        540 
HKALACGFKE VIHYVFYSKE KQQKLGFEVL EDPLELQNPI TTELNTLRTS LVCGLLDASL 

       550        560        570        580        590        600 
RNKNLGFKSI ALYEKGSVYN SKREEIQKLG FLISGLQKKE SYPDTKGKAW DFYSFAECVS 

       610        620        630        640        650        660 
KVIGDFSLEK LTTQTPINHP YQSAKIIQNH EIIGVIAKIH PKVIQELDLF ESYYAEIDAF 

       670        680        690        700        710        720 
KLKRPAMLLK PFSIYPSSVR DLTLIIDENT AFSGIKKALK DAQIPNLSEI LPLDIFKESN 

       730        740        750        760 
NSIALSVRCV IHSLEKTLND EEVNSAVQKA LEILEKEFNA RLKG

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References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J.

Venter J.C.
Nature 388:539-547(1997) [PubMed: 9252185] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07471.1.
PIR	B64570.
RefSeq	NP_207200.1. NC_000915.1.
3D structure databases
ProteinModelPortal	P56145.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3416N.
MINT	MINT-161099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	899228.
GenomeReviews	Gene locus HP_0402 in contig AE000511_GR.
KEGG	hpy:HP0402.
NMPDR	fig\|85962.1.peg.398.
PATRIC	20592043. VBIHelPyl33062_0415.
TIGR	HP_0402.
Phylogenomic databases
HOGENOM	HBG321092.
OMA	ADKLRVC.
ProtClustDB	PRK00629.
Family and domain databases
HAMAP	MF_00283. Phe_tRNA_synth_beta1. [Tree]
InterPro	IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004532. Phe-tRNA-synth_IIc_bsu_bac. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Gene3D	G3DSA:3.30.56.20. B5. 1 hit. G3DSA:3.30.70.380. Fdx_AntiC_bd. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 2 hits.
KO	K01890.
Pfam	PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. PheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_HELPY

Accession

Primary (citable) accession number: P56145

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents