tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG - Helicobacter pylori (strain ATCC 700392 / 26695)

Preferred order of sections

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	HP_0213

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm₅s₂U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 621

621

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129

PRO_0000117112

Regions

Nucleotide binding

11 – 16

6

FAD By similarity

Nucleotide binding

270 – 284

15

NAD Potential

Natural variations

Natural variant

10

1

V → I in strain: 60190.

Natural variant

105

1

L → F in strain: 60190.

Natural variant

193

1

D → E in strain: 60190.

Natural variant

220

1

T → A in strain: 60190.

Natural variant

302

1

S → N in strain: 60190.

Natural variant

353

1

T → A in strain: 60190.

Natural variant

379

1

A → D in strain: 60190.

Natural variant

415

1

I → V in strain: 60190.

Natural variant

457

1

E → Q in strain: 60190.

Natural variant

480 – 481

2

YI → CV in strain: 60190.

Natural variant

487 – 488

2

EV → KL in strain: 60190.

Natural variant

493

1

D → N in strain: 60190.

Natural variant

505

1

D → N in strain: 60190.

Sequences

Sequence

Length

Mass (Da)

Tools

P56138 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C5C5CE80259E49E8
Show »

FASTA

621

69,684

        10         20         30         40         50         60 
MVKESDILVV GGGHAGIEAS LIAAKMGARV HLITMLIDTI GLASCNPAIG GLGKGHLTKE 

        70         80         90        100        110        120 
VDVLGGAMGI ITDHSGLQYR VLNASKGPAV RGTRAQIDMD TYRILARNLV LNTPNLSVSQ 

       130        140        150        160        170        180 
EMTESLILEN DEVVGVTTNI NNTYRAKKVI ITTGTFLKGV VHIGEHQNQN GRFGENASNS 

       190        200        210        220        230        240 
LALNLRELGF KVDRLKTGTC PRVAGNSIDF EGLEEHFGDT NPPYFSYKTK DFNPTQLSCF 

       250        260        270        280        290        300 
ITYTNPITHQ IIRDNFHRAP LFSGQIEGIG PRYCPSIEDK INRFSEKERH QLFLEPQTIH 

       310        320        330        340        350        360 
KSEYYINGLS TSLPLDVQEK VIHSIKGLEN ALITRYGYAI EYDFIQPTEL THTLETKKIK 

       370        380        390        400        410        420 
GLYLAGQING TTGYEEAAAQ GLMAGINAVL ALKNQAPFIL KRNEAYIGVL IDDLITKGTN 

       430        440        450        460        470        480 
EPYRMFTSRA EYRLLLREDN TLFRLGEHAY RLGLMEEDFY KELKKDKQEI QDNLKRLKEY 

       490        500        510        520        530        540 
ILTPSKEVLK RLDELDENPI NDKVDGVSLL ARDSFNAEKM RSFFSFLAPL NERVLEQIKI 

       550        560        570        580        590        600 
ECKYNIYIEK QHENIAKMDS MLKVSIPKGF VFKGIPGLSL EAVEKLEKFR PKSLFEASEI 

       610        620 
SGITPANLDV LHLYIHLRKN S

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49503 / 60190.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07281.1. AF008565 Genomic DNA. Translation: AAB63296.1.
PIR	E64546.
RefSeq	NP_207011.1. NC_000915.1. YP_006934138.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56138.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3538N.
MINT	MINT-178352.
STRING	85962.HP0213.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07281; AAD07281; HP_0213.
GeneID	13869394. 898934.
KEGG	heo:C694_01070. hpy:HP0213.
PATRIC	20591649. VBIHelPyl33062_0224.
Phylogenomic databases
eggNOG	COG0445.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_HELPY

Accession

Primary (citable) accession number: P56138
Secondary accession number(s): O32632

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents