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P56127

- SYM_HELPY

UniProt

P56127 - SYM_HELPY

Protein

Methionine--tRNA ligase

Gene

metG

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

    Catalytic activityi

    ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi126 – 1261ZincBy similarity
    Metal bindingi129 – 1291ZincBy similarity
    Metal bindingi147 – 1471ZincBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Binding sitei304 – 3041ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciHPY:HP0417-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine--tRNA ligase (EC:6.1.1.10)
    Alternative name(s):
    Methionyl-tRNA synthetase
    Short name:
    MetRS
    Gene namesi
    Name:metG
    Ordered Locus Names:HP_0417
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 650650Methionine--tRNA ligasePRO_0000139221Add
    BLAST

    Proteomic databases

    PRIDEiP56127.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    DIPiDIP-3541N.
    IntActiP56127. 1 interaction.
    MINTiMINT-169261.
    STRINGi85962.HP0417.

    Structurei

    3D structure databases

    ProteinModelPortaliP56127.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini549 – 650102tRNA-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi11 – 2111"HIGH" regionAdd
    BLAST
    Motifi301 – 3055"KMSKS" region

    Sequence similaritiesi

    Contains 1 tRNA-binding domain.Curated

    Phylogenomic databases

    eggNOGiCOG0143.
    KOiK01874.
    OMAiQVCVVAN.
    OrthoDBiEOG6CVV9B.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_01228. Met_tRNA_synth_type2.
    InterProiIPR004495. Met-tRNA-synth_Ia_bsu_C.
    IPR014758. Met-tRNA_synth.
    IPR023457. Met-tRNA_synth_2.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR012340. NA-bd_OB-fold.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002547. tRNA-bd_dom.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PfamiPF09334. tRNA-synt_1g. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    PRINTSiPR01041. TRNASYNTHMET.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00398. metG. 1 hit.
    TIGR00399. metG_C_term. 1 hit.
    PROSITEiPS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56127-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKSLITTPI YYVNDIPHIG HAYTTLIADT LKKYYTLQGE EVFFLTGTDE    50
    HGQKIEQSAR LRNQSPKAYA DSISTIFKDQ WDFFNLDYDG FIRTTDSEHQ 100
    KCVQNAFEIM FEKGDIYKGA YSGYYCVSCE SYCAISKADN TNDKVLCPDC 150
    LRETTLLEEE SYFFRLSAYE KPLLDFYAKN PEAILPVYRK NEVTSFIEQG 200
    LLDLSITRTS FEWGIPLPKK MNDPKHVVYV WLDALLNYAS ALGYLNDLDN 250
    KMAHFECARH IVGKDILRFH AIYWPAFLMS LNLPLFKQLC VHGWWTIEGV 300
    KMSKSLGNVL DAQKIAMEYG IEELRYFLLR EVPFGQDGDF SKKALIERIN 350
    ANLNNDLGNL LNRLLGMAKK YFNHSLKSTK ITAYYSKELE KVHQILDNAN 400
    SFVPKMQLHK ALEELFNVYD FLNKLIAKEE PWVLHKNNES EKLEALLSLI 450
    ANALLQSSFL LYAFMPKSAV KLANAFNTEI TPDNYERFFK AKKLQDMILQ 500
    DTEPLFSKME KIEKTEKAGE ASPEKNEKEK KDAKEKAPLK QENYIGIEDF 550
    KKVEIKVGLI KEAQRIEKSN KLLRLKVDLG EGRLRQIISG IALDYEPESL 600
    VGQMVCVVAN LKPAKLMGEM SEGMILAVRD SDNLALISPT REKIAGSLIS 650
    Length:650
    Mass (Da):74,507
    Last modified:November 1, 1997 - v1
    Checksum:iCEFDF457B9F75DD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07483.1.
    PIRiA64572.
    RefSeqiNP_207215.1. NC_000915.1.
    YP_006934343.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07483; AAD07483; HP_0417.
    GeneIDi13869609.
    899234.
    KEGGiheo:C694_02125.
    hpy:HP0417.
    PATRICi20592085. VBIHelPyl33062_0432.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07483.1 .
    PIRi A64572.
    RefSeqi NP_207215.1. NC_000915.1.
    YP_006934343.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali P56127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3541N.
    IntActi P56127. 1 interaction.
    MINTi MINT-169261.
    STRINGi 85962.HP0417.

    Proteomic databases

    PRIDEi P56127.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07483 ; AAD07483 ; HP_0417 .
    GeneIDi 13869609.
    899234.
    KEGGi heo:C694_02125.
    hpy:HP0417.
    PATRICi 20592085. VBIHelPyl33062_0432.

    Phylogenomic databases

    eggNOGi COG0143.
    KOi K01874.
    OMAi QVCVVAN.
    OrthoDBi EOG6CVV9B.

    Enzyme and pathway databases

    BioCyci HPY:HP0417-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_01228. Met_tRNA_synth_type2.
    InterProi IPR004495. Met-tRNA-synth_Ia_bsu_C.
    IPR014758. Met-tRNA_synth.
    IPR023457. Met-tRNA_synth_2.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR012340. NA-bd_OB-fold.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002547. tRNA-bd_dom.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    Pfami PF09334. tRNA-synt_1g. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    PRINTSi PR01041. TRNASYNTHMET.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00398. metG. 1 hit.
    TIGR00399. metG_C_term. 1 hit.
    PROSITEi PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiSYM_HELPY
    AccessioniPrimary (citable) accession number: P56127
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3