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P56127 (SYM_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase

EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:HP_0417
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01228

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
tRNA-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 650650Methionine--tRNA ligase HAMAP-Rule MF_01228
PRO_0000139221

Regions

Domain549 – 650102tRNA-binding
Motif11 – 2111"HIGH" region HAMAP-Rule MF_01228
Motif301 – 3055"KMSKS" region HAMAP-Rule MF_01228

Sites

Metal binding1261Zinc By similarity
Metal binding1291Zinc By similarity
Metal binding1471Zinc By similarity
Metal binding1501Zinc By similarity
Binding site3041ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P56127 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: CEFDF457B9F75DD3

FASTA65074,507
        10         20         30         40         50         60 
MQKSLITTPI YYVNDIPHIG HAYTTLIADT LKKYYTLQGE EVFFLTGTDE HGQKIEQSAR 

        70         80         90        100        110        120 
LRNQSPKAYA DSISTIFKDQ WDFFNLDYDG FIRTTDSEHQ KCVQNAFEIM FEKGDIYKGA 

       130        140        150        160        170        180 
YSGYYCVSCE SYCAISKADN TNDKVLCPDC LRETTLLEEE SYFFRLSAYE KPLLDFYAKN 

       190        200        210        220        230        240 
PEAILPVYRK NEVTSFIEQG LLDLSITRTS FEWGIPLPKK MNDPKHVVYV WLDALLNYAS 

       250        260        270        280        290        300 
ALGYLNDLDN KMAHFECARH IVGKDILRFH AIYWPAFLMS LNLPLFKQLC VHGWWTIEGV 

       310        320        330        340        350        360 
KMSKSLGNVL DAQKIAMEYG IEELRYFLLR EVPFGQDGDF SKKALIERIN ANLNNDLGNL 

       370        380        390        400        410        420 
LNRLLGMAKK YFNHSLKSTK ITAYYSKELE KVHQILDNAN SFVPKMQLHK ALEELFNVYD 

       430        440        450        460        470        480 
FLNKLIAKEE PWVLHKNNES EKLEALLSLI ANALLQSSFL LYAFMPKSAV KLANAFNTEI 

       490        500        510        520        530        540 
TPDNYERFFK AKKLQDMILQ DTEPLFSKME KIEKTEKAGE ASPEKNEKEK KDAKEKAPLK 

       550        560        570        580        590        600 
QENYIGIEDF KKVEIKVGLI KEAQRIEKSN KLLRLKVDLG EGRLRQIISG IALDYEPESL 

       610        620        630        640        650 
VGQMVCVVAN LKPAKLMGEM SEGMILAVRD SDNLALISPT REKIAGSLIS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07483.1.
PIRA64572.
RefSeqNP_207215.1. NC_000915.1.
YP_006934343.1. NC_018939.1.

3D structure databases

ProteinModelPortalP56127.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3541N.
IntActP56127. 1 interaction.
MINTMINT-169261.
STRING85962.HP0417.

Proteomic databases

PRIDEP56127.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07483; AAD07483; HP_0417.
GeneID13869609.
899234.
KEGGheo:C694_02125.
hpy:HP0417.
PATRIC20592085. VBIHelPyl33062_0432.

Phylogenomic databases

eggNOGCOG0143.
KOK01874.
OMAITRTSFE.
OrthoDBEOG6CVV9B.
ProtClustDBPRK12267.

Enzyme and pathway databases

BioCycHPY:HP0417-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_HELPY
AccessionPrimary (citable) accession number: P56127
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries