Methionine--tRNA ligase - Helicobacter pylori (strain ATCC 700392 / 26695)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	HP_0417

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 650

650

Methionine--tRNA ligase HAMAP-Rule MF_01228

PRO_0000139221

Regions

Domain

549 – 650

102

tRNA-binding

Motif

11 – 21

11

"HIGH" region HAMAP-Rule MF_01228

Motif

301 – 305

5

"KMSKS" region HAMAP-Rule MF_01228

Sites

Metal binding

126

1

Zinc By similarity

Metal binding

129

1

Zinc By similarity

Metal binding

147

1

Zinc By similarity

Metal binding

150

1

Zinc By similarity

Binding site

304

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P56127 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: CEFDF457B9F75DD3
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FASTA

650

74,507

        10         20         30         40         50         60 
MQKSLITTPI YYVNDIPHIG HAYTTLIADT LKKYYTLQGE EVFFLTGTDE HGQKIEQSAR 

        70         80         90        100        110        120 
LRNQSPKAYA DSISTIFKDQ WDFFNLDYDG FIRTTDSEHQ KCVQNAFEIM FEKGDIYKGA 

       130        140        150        160        170        180 
YSGYYCVSCE SYCAISKADN TNDKVLCPDC LRETTLLEEE SYFFRLSAYE KPLLDFYAKN 

       190        200        210        220        230        240 
PEAILPVYRK NEVTSFIEQG LLDLSITRTS FEWGIPLPKK MNDPKHVVYV WLDALLNYAS 

       250        260        270        280        290        300 
ALGYLNDLDN KMAHFECARH IVGKDILRFH AIYWPAFLMS LNLPLFKQLC VHGWWTIEGV 

       310        320        330        340        350        360 
KMSKSLGNVL DAQKIAMEYG IEELRYFLLR EVPFGQDGDF SKKALIERIN ANLNNDLGNL 

       370        380        390        400        410        420 
LNRLLGMAKK YFNHSLKSTK ITAYYSKELE KVHQILDNAN SFVPKMQLHK ALEELFNVYD 

       430        440        450        460        470        480 
FLNKLIAKEE PWVLHKNNES EKLEALLSLI ANALLQSSFL LYAFMPKSAV KLANAFNTEI 

       490        500        510        520        530        540 
TPDNYERFFK AKKLQDMILQ DTEPLFSKME KIEKTEKAGE ASPEKNEKEK KDAKEKAPLK 

       550        560        570        580        590        600 
QENYIGIEDF KKVEIKVGLI KEAQRIEKSN KLLRLKVDLG EGRLRQIISG IALDYEPESL 

       610        620        630        640        650 
VGQMVCVVAN LKPAKLMGEM SEGMILAVRD SDNLALISPT REKIAGSLIS

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References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J.

Venter J.C.
Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07483.1.
PIR	A64572.
RefSeq	NP_207215.1. NC_000915.1. YP_006934343.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56127.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3541N.
MINT	MINT-169261.
STRING	85962.HP0417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07483; AAD07483; HP_0417.
GeneID	13869609. 899234.
KEGG	heo:C694_02125. hpy:HP0417.
PATRIC	20592085. VBIHelPyl33062_0432.
Phylogenomic databases
eggNOG	COG0143.
KO	K01874.
OMA	ITRTSFE.
ProtClustDB	PRK12267.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_01228. Met_tRNA_synth_type2. Fused.
InterPro	IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_HELPY

Accession

Primary (citable) accession number: P56127

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents