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P56127

- SYM_HELPY

UniProt

P56127 - SYM_HELPY

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Protein

Methionine--tRNA ligase

Gene
metG, HP_0417
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi126 – 1261Zinc By similarity
Metal bindingi129 – 1291Zinc By similarity
Metal bindingi147 – 1471Zinc By similarity
Metal bindingi150 – 1501Zinc By similarity
Binding sitei304 – 3041ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP0417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:HP_0417
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650Methionine--tRNA ligaseUniRule annotationPRO_0000139221Add
BLAST

Proteomic databases

PRIDEiP56127.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3541N.
IntActiP56127. 1 interaction.
MINTiMINT-169261.
STRINGi85962.HP0417.

Structurei

3D structure databases

ProteinModelPortaliP56127.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini549 – 650102tRNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionUniRule annotationAdd
BLAST
Motifi301 – 3055"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0143.
KOiK01874.
OMAiQVCVVAN.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56127-1 [UniParc]FASTAAdd to Basket

« Hide

MQKSLITTPI YYVNDIPHIG HAYTTLIADT LKKYYTLQGE EVFFLTGTDE    50
HGQKIEQSAR LRNQSPKAYA DSISTIFKDQ WDFFNLDYDG FIRTTDSEHQ 100
KCVQNAFEIM FEKGDIYKGA YSGYYCVSCE SYCAISKADN TNDKVLCPDC 150
LRETTLLEEE SYFFRLSAYE KPLLDFYAKN PEAILPVYRK NEVTSFIEQG 200
LLDLSITRTS FEWGIPLPKK MNDPKHVVYV WLDALLNYAS ALGYLNDLDN 250
KMAHFECARH IVGKDILRFH AIYWPAFLMS LNLPLFKQLC VHGWWTIEGV 300
KMSKSLGNVL DAQKIAMEYG IEELRYFLLR EVPFGQDGDF SKKALIERIN 350
ANLNNDLGNL LNRLLGMAKK YFNHSLKSTK ITAYYSKELE KVHQILDNAN 400
SFVPKMQLHK ALEELFNVYD FLNKLIAKEE PWVLHKNNES EKLEALLSLI 450
ANALLQSSFL LYAFMPKSAV KLANAFNTEI TPDNYERFFK AKKLQDMILQ 500
DTEPLFSKME KIEKTEKAGE ASPEKNEKEK KDAKEKAPLK QENYIGIEDF 550
KKVEIKVGLI KEAQRIEKSN KLLRLKVDLG EGRLRQIISG IALDYEPESL 600
VGQMVCVVAN LKPAKLMGEM SEGMILAVRD SDNLALISPT REKIAGSLIS 650
Length:650
Mass (Da):74,507
Last modified:November 1, 1997 - v1
Checksum:iCEFDF457B9F75DD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07483.1.
PIRiA64572.
RefSeqiNP_207215.1. NC_000915.1.
YP_006934343.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07483; AAD07483; HP_0417.
GeneIDi13869609.
899234.
KEGGiheo:C694_02125.
hpy:HP0417.
PATRICi20592085. VBIHelPyl33062_0432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07483.1 .
PIRi A64572.
RefSeqi NP_207215.1. NC_000915.1.
YP_006934343.1. NC_018939.1.

3D structure databases

ProteinModelPortali P56127.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3541N.
IntActi P56127. 1 interaction.
MINTi MINT-169261.
STRINGi 85962.HP0417.

Proteomic databases

PRIDEi P56127.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07483 ; AAD07483 ; HP_0417 .
GeneIDi 13869609.
899234.
KEGGi heo:C694_02125.
hpy:HP0417.
PATRICi 20592085. VBIHelPyl33062_0432.

Phylogenomic databases

eggNOGi COG0143.
KOi K01874.
OMAi QVCVVAN.
OrthoDBi EOG6CVV9B.

Enzyme and pathway databases

BioCyci HPY:HP0417-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_01228. Met_tRNA_synth_type2.
InterProi IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
Pfami PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view ]
PRINTSi PR01041. TRNASYNTHMET.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEi PS50886. TRBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiSYM_HELPY
AccessioniPrimary (citable) accession number: P56127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi