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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi126 – 1261ZincBy similarity
Metal bindingi129 – 1291ZincBy similarity
Metal bindingi147 – 1471ZincBy similarity
Metal bindingi150 – 1501ZincBy similarity
Binding sitei304 – 3041ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP0417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:HP_0417
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650Methionine--tRNA ligasePRO_0000139221Add
BLAST

Proteomic databases

PRIDEiP56127.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

DIPiDIP-3541N.
IntActiP56127. 1 interaction.
MINTiMINT-169261.
STRINGi85962.HP0417.

Structurei

3D structure databases

ProteinModelPortaliP56127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini549 – 650102tRNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi301 – 3055"KMSKS" region

Sequence similaritiesi

Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiCOG0143.
KOiK01874.
OMAiITRTSFE.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKSLITTPI YYVNDIPHIG HAYTTLIADT LKKYYTLQGE EVFFLTGTDE
60 70 80 90 100
HGQKIEQSAR LRNQSPKAYA DSISTIFKDQ WDFFNLDYDG FIRTTDSEHQ
110 120 130 140 150
KCVQNAFEIM FEKGDIYKGA YSGYYCVSCE SYCAISKADN TNDKVLCPDC
160 170 180 190 200
LRETTLLEEE SYFFRLSAYE KPLLDFYAKN PEAILPVYRK NEVTSFIEQG
210 220 230 240 250
LLDLSITRTS FEWGIPLPKK MNDPKHVVYV WLDALLNYAS ALGYLNDLDN
260 270 280 290 300
KMAHFECARH IVGKDILRFH AIYWPAFLMS LNLPLFKQLC VHGWWTIEGV
310 320 330 340 350
KMSKSLGNVL DAQKIAMEYG IEELRYFLLR EVPFGQDGDF SKKALIERIN
360 370 380 390 400
ANLNNDLGNL LNRLLGMAKK YFNHSLKSTK ITAYYSKELE KVHQILDNAN
410 420 430 440 450
SFVPKMQLHK ALEELFNVYD FLNKLIAKEE PWVLHKNNES EKLEALLSLI
460 470 480 490 500
ANALLQSSFL LYAFMPKSAV KLANAFNTEI TPDNYERFFK AKKLQDMILQ
510 520 530 540 550
DTEPLFSKME KIEKTEKAGE ASPEKNEKEK KDAKEKAPLK QENYIGIEDF
560 570 580 590 600
KKVEIKVGLI KEAQRIEKSN KLLRLKVDLG EGRLRQIISG IALDYEPESL
610 620 630 640 650
VGQMVCVVAN LKPAKLMGEM SEGMILAVRD SDNLALISPT REKIAGSLIS
Length:650
Mass (Da):74,507
Last modified:November 1, 1997 - v1
Checksum:iCEFDF457B9F75DD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07483.1.
PIRiA64572.
RefSeqiNP_207215.1. NC_000915.1.
WP_001172573.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07483; AAD07483; HP_0417.
GeneIDi899234.
KEGGiheo:C694_02125.
hpy:HP0417.
PATRICi20592085. VBIHelPyl33062_0432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07483.1.
PIRiA64572.
RefSeqiNP_207215.1. NC_000915.1.
WP_001172573.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3541N.
IntActiP56127. 1 interaction.
MINTiMINT-169261.
STRINGi85962.HP0417.

Proteomic databases

PRIDEiP56127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07483; AAD07483; HP_0417.
GeneIDi899234.
KEGGiheo:C694_02125.
hpy:HP0417.
PATRICi20592085. VBIHelPyl33062_0432.

Phylogenomic databases

eggNOGiCOG0143.
KOiK01874.
OMAiITRTSFE.
OrthoDBiEOG6CVV9B.

Enzyme and pathway databases

BioCyciHPY:HP0417-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiSYM_HELPY
AccessioniPrimary (citable) accession number: P56127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.