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Protein

Chorismate synthase

Gene

aroC

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotation1 PublicationNote: Reduced FMN (FMNH(2)).UniRule annotation1 Publication

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46NADPUniRule annotation1
Binding sitei281FMN; via amide nitrogenUniRule annotation1
Binding sitei322FMNUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 125FMNUniRule annotation1 Publication3
Nucleotide bindingi241 – 242FMNUniRule annotation2
Nucleotide bindingi296 – 300FMNUniRule annotation1 Publication5

GO - Molecular functioni

  • chorismate synthase activity Source: UniProtKB-HAMAP
  • FMN binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciHPY:HP0663-MONOMER.
BRENDAi4.2.3.5. 2604.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthase1 PublicationUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001405961 – 365Chorismate synthaseAdd BLAST365

Proteomic databases

PaxDbiP56122.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP56122. 7 interactors.
MINTiMINT-176132.
STRINGi85962.HP0663.

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Beta strandi18 – 26Combined sources9
Helixi36 – 46Combined sources11
Helixi56 – 59Combined sources4
Beta strandi62 – 65Combined sources4
Beta strandi67 – 70Combined sources4
Beta strandi79 – 84Combined sources6
Helixi106 – 113Combined sources8
Helixi126 – 145Combined sources20
Beta strandi148 – 157Combined sources10
Helixi167 – 172Combined sources6
Helixi180 – 195Combined sources16
Beta strandi202 – 212Combined sources11
Turni224 – 226Combined sources3
Helixi229 – 238Combined sources10
Beta strandi243 – 248Combined sources6
Helixi251 – 256Combined sources6
Helixi259 – 262Combined sources4
Beta strandi270 – 272Combined sources3
Turni275 – 278Combined sources4
Beta strandi289 – 295Combined sources7
Beta strandi305 – 309Combined sources5
Beta strandi314 – 317Combined sources4
Helixi328 – 349Combined sources22
Helixi350 – 352Combined sources3
Helixi355 – 362Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UM0X-ray1.95A/B/C/D1-365[»]
1UMFX-ray2.25A/B/C/D1-365[»]
ProteinModelPortaliP56122.
SMRiP56122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56122.

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
KOiK01736.
OMAiMLSINAV.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLGRFLRL TTFGESHGDV IGGVLDGMPS GIKIDYALLE NEMKRRQGGR
60 70 80 90 100
NVFITPRKED DKVEITSGVF EDFSTGTPIG FLIHNQRARS KDYDNIKNLF
110 120 130 140 150
RPSHADFTYF HKYGIRDFRG GGRSSARESA IRVAAGAFAK MLLREIGIVC
160 170 180 190 200
ESGIIEIGGI KAKNYDFNHA LKSEIFALDE EQEEAQKTAI QNAIKNHDSI
210 220 230 240 250
GGVALIRARS IKTNQKLPIG LGQGLYAKLD AKIAEAMMGL NGVKAVEIGK
260 270 280 290 300
GVESSLLKGS EYNDLMDQKG FLSNRSGGVL GGMSNGEEII VRVHFKPTPS
310 320 330 340 350
IFQPQRTIDI NGNECECLLK GRHDPCIAIR GSVVCESLLA LVLADMVLLN
360
LTSKIEYLKT IYNEN
Length:365
Mass (Da):40,100
Last modified:November 1, 1997 - v1
Checksum:i98ABC708E1909561
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07726.1.
PIRiG64602.
RefSeqiNP_207457.1. NC_000915.1.
WP_001094036.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07726; AAD07726; HP_0663.
GeneIDi898986.
KEGGiheo:C694_03430.
hpy:HP0663.
PATRICi20592611. VBIHelPyl33062_0694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07726.1.
PIRiG64602.
RefSeqiNP_207457.1. NC_000915.1.
WP_001094036.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UM0X-ray1.95A/B/C/D1-365[»]
1UMFX-ray2.25A/B/C/D1-365[»]
ProteinModelPortaliP56122.
SMRiP56122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56122. 7 interactors.
MINTiMINT-176132.
STRINGi85962.HP0663.

Proteomic databases

PaxDbiP56122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07726; AAD07726; HP_0663.
GeneIDi898986.
KEGGiheo:C694_03430.
hpy:HP0663.
PATRICi20592611. VBIHelPyl33062_0694.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
KOiK01736.
OMAiMLSINAV.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciHPY:HP0663-MONOMER.
BRENDAi4.2.3.5. 2604.

Miscellaneous databases

EvolutionaryTraceiP56122.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROC_HELPY
AccessioniPrimary (citable) accession number: P56122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.