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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ShikimateUniRule annotation3 Publications1
Active sitei69Proton acceptorUniRule annotation2 Publications1
Binding sitei90ShikimateUniRule annotation2 Publications1
Binding sitei105ShikimateUniRule annotation2 Publications1
Binding sitei181NADP; via amide nitrogen1 Publication1
Binding sitei208NADP; via carbonyl oxygenUniRule annotation1
Binding sitei210ShikimateUniRule annotation2 Publications1
Binding sitei230NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei237ShikimateUniRule annotation3 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 129NADPUniRule annotation1 Publication5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciHPY:HP1249-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:HP_1249
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360071 – 263Shikimate dehydrogenase (NADP(+))Add BLAST263

Proteomic databases

PaxDbiP56119.

Interactioni

Subunit structurei

Homodimer.UniRule annotation2 Publications

Protein-protein interaction databases

IntActiP56119. 1 interactor.
STRINGi85962.HP1249.

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 12Combined sources10
Helixi18 – 33Combined sources16
Beta strandi34 – 43Combined sources10
Beta strandi46 – 48Combined sources3
Helixi50 – 56Combined sources7
Beta strandi59 – 64Combined sources6
Helixi69 – 75Combined sources7
Beta strandi76 – 79Combined sources4
Helixi81 – 85Combined sources5
Beta strandi91 – 95Combined sources5
Beta strandi98 – 102Combined sources5
Helixi105 – 112Combined sources8
Beta strandi120 – 124Combined sources5
Helixi128 – 139Combined sources12
Beta strandi143 – 147Combined sources5
Beta strandi149 – 151Combined sources3
Helixi154 – 160Combined sources7
Beta strandi163 – 167Combined sources5
Beta strandi174 – 178Combined sources5
Helixi183 – 185Combined sources3
Helixi192 – 201Combined sources10
Beta strandi203 – 208Combined sources6
Helixi215 – 222Combined sources8
Helixi231 – 245Combined sources15
Turni246 – 248Combined sources3
Helixi252 – 262Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PHGX-ray1.57A/B1-263[»]
3PHHX-ray1.42A1-263[»]
3PHIX-ray2.04A/B1-263[»]
3PHJX-ray2.30A/B1-263[»]
4FOOX-ray2.55A/B1-263[»]
4FOSX-ray1.72A1-263[»]
4FPXX-ray2.40A/B1-263[»]
4FQ8X-ray2.07A/B1-263[»]
4FR5X-ray2.20A/B1-263[»]
4FSHX-ray2.85A/B1-263[»]
ProteinModelPortaliP56119.
SMRiP56119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 18Shikimate bindingUniRule annotation3 Publications3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiFGNPIKH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
[Graphical view]
PfamiPF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

P56119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKSFGVFG NPIKHSKSPL IHNACFLTFQ KELRFLGHYH PILLPLESHI
60 70 80 90 100
KSEFLHLGLS GANVTLPFKE RAFQVCDKIK GIALECGAVN TLVLENDELV
110 120 130 140 150
GYNTDALGFY LSLKQKNYQN ALILGAGGSA KALACELKKQ GLQVSVLNRS
160 170 180 190 200
SRGLDFFQRL GCDCFMEPPK SAFDLIINAT SASLHNELPL NKEVLKGYFK
210 220 230 240 250
EGKLAYDLAY GFLTPFLSLA KELKTPFQDG KDMLIYQAAL SFEKFSASQI
260
PYSKAFEVMR SVF
Length:263
Mass (Da):29,273
Last modified:November 1, 1997 - v1
Checksum:iA34E5BD3E54C4937
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08294.1.
PIRiA64676.
RefSeqiNP_208041.1. NC_000915.1.
WP_000769617.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08294; AAD08294; HP_1249.
GeneIDi898819.
KEGGiheo:C694_06455.
hpy:HP1249.
PATRICi20593875. VBIHelPyl33062_1308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08294.1.
PIRiA64676.
RefSeqiNP_208041.1. NC_000915.1.
WP_000769617.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PHGX-ray1.57A/B1-263[»]
3PHHX-ray1.42A1-263[»]
3PHIX-ray2.04A/B1-263[»]
3PHJX-ray2.30A/B1-263[»]
4FOOX-ray2.55A/B1-263[»]
4FOSX-ray1.72A1-263[»]
4FPXX-ray2.40A/B1-263[»]
4FQ8X-ray2.07A/B1-263[»]
4FR5X-ray2.20A/B1-263[»]
4FSHX-ray2.85A/B1-263[»]
ProteinModelPortaliP56119.
SMRiP56119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56119. 1 interactor.
STRINGi85962.HP1249.

Proteomic databases

PaxDbiP56119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08294; AAD08294; HP_1249.
GeneIDi898819.
KEGGiheo:C694_06455.
hpy:HP1249.
PATRICi20593875. VBIHelPyl33062_1308.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiFGNPIKH.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciHPY:HP1249-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
[Graphical view]
PfamiPF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_HELPY
AccessioniPrimary (citable) accession number: P56119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.