P56119 (AROE_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	HP_1249

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP-Rule MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP-Rule MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway shikimate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 263

263

Shikimate dehydrogenase HAMAP-Rule MF_00222

PRO_0000136007

Regions

Nucleotide binding

125 – 129

5

NADP By similarity

Sites

Active site

69

1

Proton acceptor Potential

Secondary structure

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263

Helix Strand Turn

Details...

Beta strand

3 – 12

10

Helix

18 – 33

16

Beta strand

34 – 43

10

Beta strand

46 – 48

3

Helix

50 – 56

7

Beta strand

59 – 64

6

Helix

69 – 75

7

Beta strand

76 – 79

4

Helix

81 – 85

5

Beta strand

91 – 95

5

Beta strand

98 – 102

5

Helix

105 – 112

8

Beta strand

120 – 124

5

Helix

128 – 139

12

Beta strand

143 – 147

5

Beta strand

149 – 151

3

Helix

154 – 160

7

Beta strand

163 – 167

5

Beta strand

174 – 178

5

Helix

183 – 185

3

Helix

192 – 201

10

Beta strand

203 – 208

6

Helix

215 – 222

8

Helix

231 – 245

15

Turn

246 – 248

3

Helix

252 – 262

11

Sequences

	Sequence		Length	Mass (Da)
	P56119 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A34E5BD3E54C4937	FASTA	263	29,273
	10 20 30 40 50 60 MKLKSFGVFG NPIKHSKSPL IHNACFLTFQ KELRFLGHYH PILLPLESHI KSEFLHLGLS 70 80 90 100 110 120 GANVTLPFKE RAFQVCDKIK GIALECGAVN TLVLENDELV GYNTDALGFY LSLKQKNYQN 130 140 150 160 170 180 ALILGAGGSA KALACELKKQ GLQVSVLNRS SRGLDFFQRL GCDCFMEPPK SAFDLIINAT 190 200 210 220 230 240 SASLHNELPL NKEVLKGYFK EGKLAYDLAY GFLTPFLSLA KELKTPFQDG KDMLIYQAAL 250 260 SFEKFSASQI PYSKAFEVMR SVF

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000511 Genomic DNA. Translation: AAD08294.1.

PIR

A64676.

RefSeq

NP_208041.1. NC_000915.1.
YP_006935169.1. NC_018939.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PHG	X-ray	1.57	A/B	1-263	[»]
3PHH	X-ray	1.42	A	1-263	[»]
3PHI	X-ray	2.04	A/B	1-263	[»]
3PHJ	X-ray	2.30	A/B	1-263	[»]
4FOO	X-ray	2.55	A/B	1-263	[»]
4FOS	X-ray	1.72	A	1-263	[»]
4FPX	X-ray	2.40	A/B	1-263	[»]
4FQ8	X-ray	2.07	A/B	1-263	[»]
4FR5	X-ray	2.20	A/B	1-263	[»]
4FSH	X-ray	2.85	A/B	1-263	[»]

ProteinModelPortal

P56119.

ModBase

Search...

Protein-protein interaction databases

STRING

85962.HP1249.

PTM databases

PhosSite

P11041007.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD08294; AAD08294; HP_1249.

GeneID

13870456.
898819.

KEGG

heo:C694_06455.
hpy:HP1249.

PATRIC

20593875. VBIHelPyl33062_1308.

Phylogenomic databases

eggNOG

COG0169.

KO

K00014.

OMA

AFDLIIN.

ProtClustDB

PRK00258.

Enzyme and pathway databases

UniPathway

UPA00053; UER00087.

Family and domain databases

Gene3D

3.40.50.720. 1 hit.

HAMAP

MF_00222. Shikimate_DH_AroE.

InterPro

IPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]

Pfam

PF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00507. aroE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

AROE_HELPY

Accession

Primary (citable) accession number: P56119

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program