ID   DPO1_HELPY              Reviewed;         891 AA.
AC   P56105;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   27-MAR-2024, entry version 142.
DE   RecName: Full=DNA polymerase I;
DE            Short=POL I;
DE            EC=2.7.7.7;
GN   Name=polA; OrderedLocusNames=HP_1470;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 3'-5' and 5'-3' exonuclease activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: Single-chain monomer with multiple functions.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD08510.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE000511; AAD08510.1; ALT_INIT; Genomic_DNA.
DR   PIR; F64703; F64703.
DR   RefSeq; NP_208261.1; NC_000915.1.
DR   RefSeq; WP_000437576.1; NC_018939.1.
DR   AlphaFoldDB; P56105; -.
DR   SMR; P56105; -.
DR   DIP; DIP-3098N; -.
DR   IntAct; P56105; 5.
DR   MINT; P56105; -.
DR   STRING; 85962.HP_1470; -.
DR   PaxDb; 85962-C694_07610; -.
DR   EnsemblBacteria; AAD08510; AAD08510; HP_1470.
DR   KEGG; hpy:HP_1470; -.
DR   PATRIC; fig|85962.47.peg.1581; -.
DR   eggNOG; COG0258; Bacteria.
DR   eggNOG; COG0749; Bacteria.
DR   InParanoid; P56105; -.
DR   OrthoDB; 9806424at2; -.
DR   PhylomeDB; P56105; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR   CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   CDD; cd09859; PIN_53EXO; 1.
DR   Gene3D; 3.30.70.370; -; 1.
DR   Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR   Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR   Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR002421; 5-3_exonuclease.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   NCBIfam; TIGR00593; pola; 1.
DR   PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR   PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   SUPFAM; SSF88723; PIN domain-like; 1.
DR   SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..891
FT                   /note="DNA polymerase I"
FT                   /id="PRO_0000101241"
FT   DOMAIN          1..313
FT                   /note="5'-3' exonuclease"
FT   DOMAIN          314..488
FT                   /note="3'-5' exonuclease"
FT   REGION          492..890
FT                   /note="Polymerase"
SQ   SEQUENCE   891 AA;  101975 MW;  3D6E7D8FF613EB88 CRC64;
     MEQPVIKEGT LALIDTFAYL FRSYYMSAKN KPLTNDKGFP TGLLTGLVGM VKKFYKDRKN
     MPFIVFALES QTKTKRAEKL GEYKQNRKDA PKEMLLQIPI ALEWLQKMGF VCVEVNGFEA
     DDVIASLATL SPYKTRIYSK DKDFNQLLSD KIALFDGKTE FLAKDCVEKY GILPSQFTDY
     QGIVGDSSDN YKGVKGIGSK NAKELLQRLG SLEKIYENLD LAKNLLSPKM YRALIHDKAS
     AFLSKELATL ERGCIKEFDF LSCAFPSENP LLKIKDELKE YGFISTLRDL ENSPTPLILD
     NAPLLDNTPA LDNTPKKSCM IVLESAAPLS AFLEKLEKTN ARVFARLVLD KEKKVLALAF
     LYEDQGYFLP LEEALFSPFS LEFLQNAFFK MLQHAQIIGH DLKPLLSFLK AKYQVPLENI
     RIQDTQILAF LKNPEKVGFD EVLKEYLKEE LIPHEKIKDF KTKAEKLELL SVELNALKRL
     CEYFEKGGLE ENLLSLAREI ETPFMKVLMG MEFQGFKIDA PYFKRLEQEF KNELHVLERQ
     ILELIGVDFN LNSPKQLSEV LYDKLGLPKN KSHSTDEKSL LKILDKHPSI ALILEYRELN
     KLFNTYTTPL LRLKDKDDKI HTTFIQTGTA TGRLSSHSPN LQNIPVRSPK GLLIRKGFIA
     SSKEYCLLGV DYSQIELRLL AHFSQDKDLM EAFLKGRDIH LETSKALFGE YLAKEKRSIA
     KSINFGLVYG MGSKKLSETL NISLNEAKSY IEAYFKRFPS IKDYLNRMKE EILKTSKAFT
     LLGRYRVFDF TGANDYVKGN YLREGVNAIF QGSASDLLKL GMLKVSERFK NNPSVRLLLQ
     VHDELIFEIE EKNAPELQQE IQRILNDEVY PLRVPLETSA FIAKRWNELK G
//