Reviewed, UniProtKB/Swiss-Prot P56102 (AMPM_HELPY)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	HP_1299

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Methionine aminopeptidase		PRO_0000148941
Sites
	Metal binding	95	1	Cobalt 1 By similarity
	Metal binding	106	1	Cobalt 1 By similarity
	Metal binding	106	1	Cobalt 2 By similarity
	Metal binding	169	1	Cobalt 2 By similarity
	Metal binding	206	1	Cobalt 2 By similarity
	Metal binding	237	1	Cobalt 1 By similarity
	Metal binding	237	1	Cobalt 2 By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56102-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C09772EB85A6A8DA	FASTA	253	27,577
	10 20 30 40 50 60 MAISIKSPKE IKALRKAGEL TAQALALLER EVRPGVSLLE LDKMAEDFIK SSHARPAFKG 70 80 90 100 110 120 LYGFPNSVCM SLNEVVIHGI PTDYVLQEGD IIGLDLGVEV DGYYGDSALT LPIGAISPQD 130 140 150 160 170 180 EKLLACSKES LMHAINSIRV GMHFKELSQI LESTITERGF VPLKGFCGHG IGKKPHEEPE 190 200 210 220 230 240 IPNYLEKGVK PNSGPKIKEG MVFCLEPMVC QKQGEPKILA DKWSVVSVDG LNTSHHEHTI 250 AIVGNKAVIL TER

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD08340.1.
PIR	C64682.
RefSeq	NP_208091.1.
3D structure databases
HSSP	HSSP built from PDB template 1C24 based on UniProtKB P07906.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3487N.
Genome annotation databases
GeneID	898824.
GenomeReviews	Gene locus HP_1299 in contig AE000511_GR.
KEGG	hpy:HP1299.
NMPDR	fig|85962.1.peg.1287.
TIGR	HP_1299.
Phylogenomic databases
HOGENOM	P56102.
OMA	TATGPRI.
Enzyme and pathway databases
BRENDA	3.4.11.18. 1131.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_HELPY

Accession

Primary (citable) accession number: P56102

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)