Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome bd-I ubiquinol oxidase subunit X

Gene

cydX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for correct functioning of cytochrome bd-I oxidase. This protein and AppX may have some functional overlap.

Catalytic activityi

2 ubiquinol + O2 + 4 H+(Side 1) = 2 ubiquinone + 2 H2O + 4 H+(Side 2).

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

GO - Molecular functioni

  • oxidoreductase activity, acting on diphenols and related substances as donors Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2663.
ECOL316407:JW0724-MONOMER.
MetaCyc:MONOMER0-2663.
UniPathwayiUPA00705.

Protein family/group databases

TCDBi9.B.85.1.1. the 1 tms cydx/appx cytochrome bd oxidase activating (cydx/appx) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bd-I ubiquinol oxidase subunit X (EC:1.10.3.14)
Alternative name(s):
Cytochrome bd-I oxidase subunit X
Cytochrome d ubiquinol oxidase subunit X
Gene namesi
Name:cydX
Synonyms:ybgT
Ordered Locus Names:b4515, JW0724
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14329. cydX.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: EcoCyc
  • outer membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Slow growth in aerobic liquid culture, forms mixed colonies on aerobic solid media, increased sensitivity to reductant (beta-mercapoethanol). Reduced cytochrome bd-I oxidase activity.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 11M → Q: Does not restore reductant (beta-mercapoethanol) resistance. 1 Publication
Mutagenesisi4 – 41F → A: Does not restore reductant (beta-mercapoethanol) resistance. 1 Publication
Mutagenesisi7 – 71I → A: Does not restore reductant (beta-mercapoethanol) resistance. 1 Publication
Mutagenesisi12 – 121L → A: Does not restore reductant (beta-mercapoethanol) resistance. 1 Publication
Mutagenesisi14 – 141C → S: Restores reductant (beta-mercapoethanol) resistance. 1 Publication
Mutagenesisi21 – 211A → G: Does not restore reductant (beta-mercapoethanol) resistance. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3737Cytochrome bd-I ubiquinol oxidase subunit XPRO_0000168701Add
BLAST

Proteomic databases

PaxDbiP56100.

Expressioni

Inductioni

Constitutively expressed during aerobic growth (PubMed:19121005), induced in low oxygen, and maybe by SDS/EDTA (envelope stress) (PubMed:19734316) (at protein level).2 Publications

Interactioni

Subunit structurei

May be a subunit of cytochrome bd-I ubiquinol oxidase. Probably interacts with CydA and CydB.1 Publication

Protein-protein interaction databases

BioGridi4259450. 12 interactions.
IntActiP56100. 2 interactions.
STRINGi511145.b4515.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4890. LUCA.
HOGENOMiHOG000264864.
OrthoDBiEOG6677XQ.

Family and domain databases

InterProiIPR011724. Cyd_oper_YbgT.
IPR012994. YbgT_YccB.
[Graphical view]
PfamiPF08173. YbgT_YccB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02106. cyd_oper_ybgT. 1 hit.

Sequencei

Sequence statusi: Complete.

P56100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
MWYFAWILGT LLACSFGVIT ALALEHVESG KAGQEDI
Length:37
Mass (Da):4,042
Last modified:November 1, 1997 - v1
Checksum:iFCA4CC0A727F668B
GO

Sequence cautioni

The sequence J03939 differs from that shown. Reason: Frameshift at positions 22 and 27. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03939 Unassigned DNA. No translation available.
U30934 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: ABD18645.1.
AP009048 Genomic DNA. Translation: BAE76359.1.
RefSeqiWP_000270282.1. NZ_LN832404.1.
YP_588444.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiABD18645; ABD18645; b4515.
BAE76359; BAE76359; BAE76359.
GeneIDi1450246.
KEGGiecj:JW0724.
eco:b4515.
PATRICi32116667. VBIEscCol129921_0766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03939 Unassigned DNA. No translation available.
U30934 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: ABD18645.1.
AP009048 Genomic DNA. Translation: BAE76359.1.
RefSeqiWP_000270282.1. NZ_LN832404.1.
YP_588444.1. NC_000913.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259450. 12 interactions.
IntActiP56100. 2 interactions.
STRINGi511145.b4515.

Protein family/group databases

TCDBi9.B.85.1.1. the 1 tms cydx/appx cytochrome bd oxidase activating (cydx/appx) family.

Proteomic databases

PaxDbiP56100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD18645; ABD18645; b4515.
BAE76359; BAE76359; BAE76359.
GeneIDi1450246.
KEGGiecj:JW0724.
eco:b4515.
PATRICi32116667. VBIEscCol129921_0766.

Organism-specific databases

EchoBASEiEB4075.
EcoGeneiEG14329. cydX.

Phylogenomic databases

eggNOGiCOG4890. LUCA.
HOGENOMiHOG000264864.
OrthoDBiEOG6677XQ.

Enzyme and pathway databases

UniPathwayiUPA00705.
BioCyciEcoCyc:MONOMER0-2663.
ECOL316407:JW0724-MONOMER.
MetaCyc:MONOMER0-2663.

Miscellaneous databases

PROiP56100.

Family and domain databases

InterProiIPR011724. Cyd_oper_YbgT.
IPR012994. YbgT_YccB.
[Graphical view]
PfamiPF08173. YbgT_YccB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02106. cyd_oper_ybgT. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coli."
    Green G.N., Fang H., Lin R.-J., Newton G., Mather M., Georgiou C.D., Gennis R.B.
    J. Biol. Chem. 263:13138-13143(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Characterization of the tol-pal and cyd region of Escherichia coli K-12: transcript analysis and identification of two new proteins encoded by the cyd operon."
    Muller M.M., Webster R.E.
    J. Bacteriol. 179:2077-2080(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Small membrane proteins found by comparative genomics and ribosome binding site models."
    Hemm M.R., Paul B.J., Schneider T.D., Storz G., Rudd K.E.
    Mol. Microbiol. 70:1487-1501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  8. "Small stress response proteins in Escherichia coli: proteins missed by classical proteomic studies."
    Hemm M.R., Paul B.J., Miranda-Rios J., Zhang A., Soltanzad N., Storz G.
    J. Bacteriol. 192:46-58(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY STRESS.
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Membrane localization of small proteins in Escherichia coli."
    Fontaine F., Fuchs R.T., Storz G.
    J. Biol. Chem. 286:32464-32474(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
    Strain: K12 / MG1655 / ATCC 47076.
  10. "The Escherichia coli CydX protein is a member of the CydAB cytochrome bd oxidase complex and is required for cytochrome bd oxidase activity."
    Vanorsdel C.E., Bhatt S., Allen R.J., Brenner E.P., Hobson J.J., Jamil A., Haynes B.M., Genson A.M., Hemm M.R.
    J. Bacteriol. 195:3640-3650(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE INTERACTION WITH CYDA AND CYDB, SUBUNIT, DISRUPTION PHENOTYPE, MUTAGENESIS OF MET-1; PHE-4; ILE-7; LEU-12; CYS-14 AND ALA-21.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCYDX_ECOLI
AccessioniPrimary (citable) accession number: P56100
Secondary accession number(s): Q2EER0, Q2MBJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.