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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K(+)UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei244 – 2441NADUniRule annotation
Metal bindingi295 – 2951Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi297 – 2971Potassium; via carbonyl oxygenUniRule annotation
Binding sitei298 – 2981IMPUniRule annotation
Active sitei300 – 3001Thioimidate intermediateUniRule annotation
Metal bindingi300 – 3001Potassium; via carbonyl oxygenUniRule annotation
Binding sitei410 – 4101IMPUniRule annotation
Metal bindingi464 – 4641Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi465 – 4651Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi466 – 4661Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi293 – 2953NADUniRule annotation

GO - Molecular functioni

  1. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. nucleotide binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciHPY:HP0829-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:HP_0829
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Inosine-5'-monophosphate dehydrogenasePRO_0000093698Add
BLAST

Proteomic databases

PRIDEiP56088.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi85962.HP0829.

Structurei

3D structure databases

ProteinModelPortaliP56088.
SMRiP56088. Positions 2-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini92 – 14857CBS 1UniRule annotationAdd
BLAST
Domaini152 – 20958CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni333 – 3353IMP bindingUniRule annotation
Regioni356 – 3572IMP bindingUniRule annotation
Regioni380 – 3845IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
KOiK00088.
OMAiSSMGYCG.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56088-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILQRALTF EDVLMVPRKS SVLPKDVSLK SRLTKNIRLN IPFISAAMDT
60 70 80 90 100
VTEHKTAIAM ARLGGIGIVH KNMDIQTQVK EITKVKKSES GVINDPIFIH
110 120 130 140 150
AHRTLADAKV ITDNYKISGV PVVDDKGLLI GILTNRDVRF ETDLSKKVGD
160 170 180 190 200
VMTKMPLVTA HVGISLDEAS DLMHKHKIEK LPIVDKDNVL KGLITIKDIQ
210 220 230 240 250
KRIEYPEANK DDFGRLRVGA AIGVGQLDRA EMLVKAGVDA LVLDSAHGHS
260 270 280 290 300
ANILHTLEEI KKSLVVDVIV GNVVTKEATS DLISAGADAI KVGIGPGSIC
310 320 330 340 350
TTRIVAGVGM PQVSAIDNCV EVASKFDIPV IADGGIRYSG DVAKALALGA
360 370 380 390 400
SSVMIGSLLA GTEESPGDFM IYQGRQYKSY RGMGSIGAMT KGSSDRYFQE
410 420 430 440 450
GVASEKLVPE GIEGRVPYRG KVSDMIFQLV GGVRSSMGYQ GAKNILELYQ
460 470 480
NAEFVEITSA GLKESHVHGV DITKEAPNYY G
Length:481
Mass (Da):51,802
Last modified:November 1, 1997 - v1
Checksum:i075A84B1F8AC9481
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07879.1.
PIRiE64623.
RefSeqiNP_207622.1. NC_000915.1.
YP_006934744.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07879; AAD07879; HP_0829.
GeneIDi13870013.
900202.
KEGGiheo:C694_04250.
hpy:HP0829.
PATRICi20592949. VBIHelPyl33062_0863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07879.1.
PIRiE64623.
RefSeqiNP_207622.1. NC_000915.1.
YP_006934744.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56088.
SMRiP56088. Positions 2-480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0829.

Proteomic databases

PRIDEiP56088.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07879; AAD07879; HP_0829.
GeneIDi13870013.
900202.
KEGGiheo:C694_04250.
hpy:HP0829.
PATRICi20592949. VBIHelPyl33062_0863.

Phylogenomic databases

eggNOGiCOG0517.
KOiK00088.
OMAiSSMGYCG.
OrthoDBiEOG6GTZPV.

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.
BioCyciHPY:HP0829-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiIMDH_HELPY
AccessioniPrimary (citable) accession number: P56088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 4, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.