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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (guaB)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei244NADUniRule annotation1
Metal bindingi295Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi297Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei298IMPUniRule annotation1
Active sitei300Thioimidate intermediateUniRule annotation1
Metal bindingi300Potassium; via carbonyl oxygenUniRule annotation1
Active sitei396Proton acceptorUniRule annotation1
Binding sitei410IMPUniRule annotation1
Metal bindingi464Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi465Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi466Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi293 – 295NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciHPY:HP0829-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:HP_0829
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000936981 – 481Inosine-5'-monophosphate dehydrogenaseAdd BLAST481

Proteomic databases

PaxDbiP56088.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi85962.HP0829.

Structurei

3D structure databases

ProteinModelPortaliP56088.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 148CBS 1UniRule annotationAdd BLAST57
Domaini152 – 209CBS 2UniRule annotationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni333 – 335IMP bindingUniRule annotation3
Regioni356 – 357IMP bindingUniRule annotation2
Regioni380 – 384IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiENOG4105CP4. Bacteria.
COG0516. LUCA.
COG0517. LUCA.
KOiK00088.
OMAiSSMGYCG.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56088-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILQRALTF EDVLMVPRKS SVLPKDVSLK SRLTKNIRLN IPFISAAMDT
60 70 80 90 100
VTEHKTAIAM ARLGGIGIVH KNMDIQTQVK EITKVKKSES GVINDPIFIH
110 120 130 140 150
AHRTLADAKV ITDNYKISGV PVVDDKGLLI GILTNRDVRF ETDLSKKVGD
160 170 180 190 200
VMTKMPLVTA HVGISLDEAS DLMHKHKIEK LPIVDKDNVL KGLITIKDIQ
210 220 230 240 250
KRIEYPEANK DDFGRLRVGA AIGVGQLDRA EMLVKAGVDA LVLDSAHGHS
260 270 280 290 300
ANILHTLEEI KKSLVVDVIV GNVVTKEATS DLISAGADAI KVGIGPGSIC
310 320 330 340 350
TTRIVAGVGM PQVSAIDNCV EVASKFDIPV IADGGIRYSG DVAKALALGA
360 370 380 390 400
SSVMIGSLLA GTEESPGDFM IYQGRQYKSY RGMGSIGAMT KGSSDRYFQE
410 420 430 440 450
GVASEKLVPE GIEGRVPYRG KVSDMIFQLV GGVRSSMGYQ GAKNILELYQ
460 470 480
NAEFVEITSA GLKESHVHGV DITKEAPNYY G
Length:481
Mass (Da):51,802
Last modified:November 1, 1997 - v1
Checksum:i075A84B1F8AC9481
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07879.1.
PIRiE64623.
RefSeqiNP_207622.1. NC_000915.1.
WP_001221712.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07879; AAD07879; HP_0829.
GeneIDi900202.
KEGGiheo:C694_04250.
hpy:HP0829.
PATRICi20592949. VBIHelPyl33062_0863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07879.1.
PIRiE64623.
RefSeqiNP_207622.1. NC_000915.1.
WP_001221712.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56088.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0829.

Proteomic databases

PaxDbiP56088.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07879; AAD07879; HP_0829.
GeneIDi900202.
KEGGiheo:C694_04250.
hpy:HP0829.
PATRICi20592949. VBIHelPyl33062_0863.

Phylogenomic databases

eggNOGiENOG4105CP4. Bacteria.
COG0516. LUCA.
COG0517. LUCA.
KOiK00088.
OMAiSSMGYCG.

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.
BioCyciHPY:HP0829-MONOMER.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMDH_HELPY
AccessioniPrimary (citable) accession number: P56088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 5, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.