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Protein

3-dehydroquinate synthase

Gene

aroB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • NAD+By similarity
  • Co2+By similarity, Zn2+By similarityNote: Binds 1 divalent metal cation per subunit. Can use either Co2+ or Zn2+.By similarity

Pathwayi: chorismate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

Cobalt, NAD, Zinc

Enzyme and pathway databases

BioCyciHPY:HP0283-MONOMER.
BRENDAi4.2.3.4. 2604.
UniPathwayiUPA00053; UER00085.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate synthase (EC:4.2.3.4)
Gene namesi
Name:aroB
Ordered Locus Names:HP_0283
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3433433-dehydroquinate synthasePRO_0000140745Add
BLAST

Proteomic databases

PaxDbiP56081.
PRIDEiP56081.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP56081. 1 interaction.
STRINGi85962.HP0283.

Structurei

Secondary structure

1
343
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 123Combined sources
Beta strandi14 – 196Combined sources
Beta strandi29 – 346Combined sources
Helixi35 – 384Combined sources
Helixi42 – 465Combined sources
Beta strandi54 – 596Combined sources
Helixi63 – 653Combined sources
Helixi68 – 8013Combined sources
Beta strandi88 – 947Combined sources
Helixi95 – 10713Combined sources
Beta strandi113 – 1186Combined sources
Helixi121 – 1255Combined sources
Beta strandi128 – 1303Combined sources
Beta strandi132 – 1376Combined sources
Beta strandi140 – 1478Combined sources
Beta strandi151 – 1555Combined sources
Helixi160 – 1623Combined sources
Helixi165 – 18218Combined sources
Helixi184 – 1929Combined sources
Turni195 – 1973Combined sources
Helixi199 – 21315Combined sources
Turni225 – 2306Combined sources
Helixi231 – 24010Combined sources
Turni241 – 2433Combined sources
Helixi248 – 26518Combined sources
Helixi271 – 28313Combined sources
Beta strandi320 – 3223Combined sources
Helixi332 – 3387Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CLHX-ray2.40A/B1-343[»]
ProteinModelPortaliP56081.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56081.

Family & Domainsi

Sequence similaritiesi

Belongs to the dehydroquinate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105D49. Bacteria.
COG0337. LUCA.
KOiK01735.
OMAiVAIGMNM.
OrthoDBiEOG6SJJGD.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase.
InterProiIPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
[Graphical view]
PfamiPF01761. DHQ_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.

Sequencei

Sequence statusi: Complete.

P56081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEILIPLKE KNYKVFLGEL PEIKLKQKAL IISDSIVAGL HLPYLLERLK
60 70 80 90 100
ALEVRVCVIE SGEKYKNFHS LERILNNAFE MQLNRHSLMI ALGGGVISDM
110 120 130 140 150
VGFASSIYFR GIDFINIPTT LLAQVDASVG GKTGINTPYG KNLIGSFHQP
160 170 180 190 200
KAVYMDLAFL KTLEKREFQA GVAEIIKMAV CFDKNLVERL ETKDLKDCLE
210 220 230 240 250
EVIFQSVNIK AQVVVQDEKE QNIRAGLNYG HTFGHAIEKE TDYERFLHGE
260 270 280 290 300
AIAIGMRMAN DLALSLGMLT LKEYERIENL LKKFDLIFHY KILDLQKFYE
310 320 330 340
RLFLDKKSEN KTIKFILPKG VGAFEVASHI PKETIIKVLE KWH
Length:343
Mass (Da):39,120
Last modified:November 1, 1997 - v1
Checksum:i5AEAC2F4DE816D13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07351.1.
PIRiC64555.
RefSeqiNP_207081.1. NC_000915.1.
WP_001156090.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07351; AAD07351; HP_0283.
GeneIDi900113.
KEGGiheo:C694_01430.
hpy:HP0283.
PATRICi20591793. VBIHelPyl33062_0294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07351.1.
PIRiC64555.
RefSeqiNP_207081.1. NC_000915.1.
WP_001156090.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CLHX-ray2.40A/B1-343[»]
ProteinModelPortaliP56081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56081. 1 interaction.
STRINGi85962.HP0283.

Proteomic databases

PaxDbiP56081.
PRIDEiP56081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07351; AAD07351; HP_0283.
GeneIDi900113.
KEGGiheo:C694_01430.
hpy:HP0283.
PATRICi20591793. VBIHelPyl33062_0294.

Phylogenomic databases

eggNOGiENOG4105D49. Bacteria.
COG0337. LUCA.
KOiK01735.
OMAiVAIGMNM.
OrthoDBiEOG6SJJGD.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00085.
BioCyciHPY:HP0283-MONOMER.
BRENDAi4.2.3.4. 2604.

Miscellaneous databases

EvolutionaryTraceiP56081.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase.
InterProiIPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
[Graphical view]
PfamiPF01761. DHQ_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiAROB_HELPY
AccessioniPrimary (citable) accession number: P56081
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 11, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.