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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation1 Publication

Kineticsi

Kcat is 88000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at pH 7.5 and 25 degrees Celsius).1 Publication
  1. KM=3.46 µM for D-glyceraldehyde 3-phosphate (at pH 7.5 and 25 degrees Celsius)1 Publication

    Pathwayi: gluconeogenesis

    This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

    Pathwayi: glycolysis

    This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Triosephosphate isomerase (tpiA)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei90ElectrophileUniRule annotation1
    Active sitei159Proton acceptorUniRule annotation1
    Binding sitei165Substrate; via amide nitrogenUniRule annotation1 Publication1
    Binding sitei197SubstrateUniRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processGluconeogenesis, Glycolysis

    Enzyme and pathway databases

    BioCyciHPY:HP0194-MONOMER
    BRENDAi5.3.1.1 2604
    SABIO-RKiP56076
    UniPathwayiUPA00109; UER00189
    UPA00138

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triosephosphate isomerase1 PublicationUniRule annotation (EC:5.3.1.1UniRule annotation1 Publication)
    Short name:
    TIM1 PublicationUniRule annotation
    Short name:
    TPIUniRule annotation
    Alternative name(s):
    Triose-phosphate isomerase1 PublicationUniRule annotation
    Gene namesi
    Name:tpiAUniRule annotation
    Synonyms:tpi
    Ordered Locus Names:HP_0194
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    Proteomesi
    • UP000000429 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi183K → A: 3-fold decrease of the affinity and slight decrease of the catalytic efficiency. 1 Publication1
    Mutagenesisi183K → S: The affinity and the catalytic efficiency are comparable to the wild-type. 1 Publication1
    Mutagenesisi213D → A: The affinity and the catalytic efficiency are comparable to the wild-type. 1 Publication1
    Mutagenesisi213D → Q: The affinity and the catalytic efficiency are comparable to the wild-type. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000902281 – 234Triosephosphate isomeraseAdd BLAST234

    Proteomic databases

    PaxDbiP56076

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    DIPiDIP-3273N
    IntActiP56076, 5 interactors
    MINTiP56076
    STRINGi85962.HP0194

    Structurei

    Secondary structure

    1234
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 8Combined sources5
    Helixi15 – 28Combined sources14
    Helixi31 – 33Combined sources3
    Turni34 – 36Combined sources3
    Beta strandi37 – 40Combined sources4
    Turni43 – 45Combined sources3
    Beta strandi52 – 56Combined sources5
    Beta strandi62 – 68Combined sources7
    Helixi75 – 80Combined sources6
    Beta strandi85 – 88Combined sources4
    Helixi91 – 95Combined sources5
    Helixi101 – 113Combined sources13
    Beta strandi117 – 122Combined sources6
    Helixi126 – 131Combined sources6
    Helixi133 – 144Combined sources12
    Beta strandi154 – 158Combined sources5
    Helixi161 – 163Combined sources3
    Helixi172 – 185Combined sources14
    Beta strandi192 – 197Combined sources6
    Turni200 – 202Combined sources3
    Helixi203 – 207Combined sources5
    Beta strandi214 – 218Combined sources5
    Helixi219 – 222Combined sources4
    Helixi224 – 231Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2JGQX-ray2.30A/B2-234[»]
    ProteinModelPortaliP56076
    SMRiP56076
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56076

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni8 – 10Substrate bindingUniRule annotation1 Publication3
    Regioni218 – 219Substrate bindingUniRule annotation1 Publication2

    Sequence similaritiesi

    Belongs to the triosephosphate isomerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105CP7 Bacteria
    COG0149 LUCA
    KOiK01803
    OMAiLCVGEGL

    Family and domain databases

    CDDicd00311 TIM, 1 hit
    Gene3Di3.20.20.70, 1 hit
    HAMAPiMF_00147_B TIM_B, 1 hit
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR035990 TIM_sf
    IPR022896 TrioseP_Isoase_bac/euk
    IPR000652 Triosephosphate_isomerase
    IPR020861 Triosephosphate_isomerase_AS
    PANTHERiPTHR21139 PTHR21139, 1 hit
    PfamiView protein in Pfam
    PF00121 TIM, 1 hit
    SUPFAMiSSF51351 SSF51351, 1 hit
    PROSITEiView protein in PROSITE
    PS00171 TIM_1, 1 hit
    PS51440 TIM_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P56076-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTKIAMANFK SAMPIFKSHA YLKELEKTLK PQHFDRVFVF PDFFGLLPNS
    60 70 80 90 100
    FLHFTLGVQN AYPRDCGAFT GEITSKHLEE LKIHTLLIGH SERRTLLKES
    110 120 130 140 150
    PSFLKEKFDF FKSKNFKIVY CIGEELTTRE KGFKAVKEFL SEQLENIDLN
    160 170 180 190 200
    YPNLVVAYEP IWAIGTKKSA SLEDIYLTHG FLKQILNQKT PLLYGGSVNT
    210 220 230
    QNAKEILGID SVDGLLIGSA SWELENFKTI ISFL
    Length:234
    Mass (Da):26,708
    Last modified:November 1, 1997 - v1
    Checksum:iAFEB4FF92D5BC4D3
    GO

    Mass spectrometryi

    Molecular mass is 28108 Da from positions 1 - 234. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA Translation: AAD07261.1
    PIRiB64544
    RefSeqiNP_206993.1, NC_000915.1
    WP_000160988.1, NC_018939.1

    Genome annotation databases

    EnsemblBacteriaiAAD07261; AAD07261; HP_0194
    GeneIDi899983
    KEGGiheo:C694_00965
    hpy:HP0194
    PATRICifig|85962.47.peg.209

    Similar proteinsi

    Entry informationi

    Entry nameiTPIS_HELPY
    AccessioniPrimary (citable) accession number: P56076
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: April 25, 2018
    This is version 114 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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