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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation1 Publication

Kineticsi

Kcat is 88000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at pH 7.5 and 25 degrees Celsius).1 Publication

Manual assertion based on experiment ini

  1. KM=3.46 µM for D-glyceraldehyde 3-phosphate (at pH 7.5 and 25 degrees Celsius)1 Publication

    Pathwayi: gluconeogenesis

    This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

    Pathwayi: glycolysis

    This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Triosephosphate isomerase (tpiA)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei90ElectrophileUniRule annotation1
    Active sitei159Proton acceptorUniRule annotation1
    Binding sitei165Substrate; via amide nitrogenUniRule annotation1 Publication1
    Binding sitei197SubstrateUniRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Gluconeogenesis, Glycolysis, Pentose shunt

    Enzyme and pathway databases

    BioCyciHPY:HP0194-MONOMER.
    BRENDAi5.3.1.1. 2604.
    SABIO-RKP56076.
    UniPathwayiUPA00109; UER00189.
    UPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triosephosphate isomerase1 PublicationUniRule annotation (EC:5.3.1.1UniRule annotation1 Publication)
    Short name:
    TIM1 PublicationUniRule annotation
    Short name:
    TPIUniRule annotation
    Alternative name(s):
    Triose-phosphate isomerase1 PublicationUniRule annotation
    Gene namesi
    Name:tpiAUniRule annotation
    Synonyms:tpi
    Ordered Locus Names:HP_0194
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    Proteomesi
    • UP000000429 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi183K → A: 3-fold decrease of the affinity and slight decrease of the catalytic efficiency. 1 Publication1
    Mutagenesisi183K → S: The affinity and the catalytic efficiency are comparable to the wild-type. 1 Publication1
    Mutagenesisi213D → A: The affinity and the catalytic efficiency are comparable to the wild-type. 1 Publication1
    Mutagenesisi213D → Q: The affinity and the catalytic efficiency are comparable to the wild-type. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000902281 – 234Triosephosphate isomeraseAdd BLAST234

    Proteomic databases

    PaxDbiP56076.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    DIPiDIP-3273N.
    IntActiP56076. 5 interactors.
    MINTiMINT-166065.
    STRINGi85962.HP0194.

    Structurei

    Secondary structure

    1234
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 8Combined sources5
    Helixi15 – 28Combined sources14
    Helixi31 – 33Combined sources3
    Turni34 – 36Combined sources3
    Beta strandi37 – 40Combined sources4
    Turni43 – 45Combined sources3
    Beta strandi52 – 56Combined sources5
    Beta strandi62 – 68Combined sources7
    Helixi75 – 80Combined sources6
    Beta strandi85 – 88Combined sources4
    Helixi91 – 95Combined sources5
    Helixi101 – 113Combined sources13
    Beta strandi117 – 122Combined sources6
    Helixi126 – 131Combined sources6
    Helixi133 – 144Combined sources12
    Beta strandi154 – 158Combined sources5
    Helixi161 – 163Combined sources3
    Helixi172 – 185Combined sources14
    Beta strandi192 – 197Combined sources6
    Turni200 – 202Combined sources3
    Helixi203 – 207Combined sources5
    Beta strandi214 – 218Combined sources5
    Helixi219 – 222Combined sources4
    Helixi224 – 231Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2JGQX-ray2.30A/B2-234[»]
    ProteinModelPortaliP56076.
    SMRiP56076.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56076.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni8 – 10Substrate bindingUniRule annotation1 Publication3
    Regioni218 – 219Substrate bindingUniRule annotation1 Publication2

    Sequence similaritiesi

    Belongs to the triosephosphate isomerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105CP7. Bacteria.
    COG0149. LUCA.
    KOiK01803.
    OMAiPNSFLHF.

    Family and domain databases

    CDDicd00311. TIM. 1 hit.
    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00147_B. TIM_B. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR022896. TrioseP_Isoase_bac/euk.
    IPR000652. Triosephosphate_isomerase.
    IPR020861. Triosephosphate_isomerase_AS.
    [Graphical view]
    PANTHERiPTHR21139. PTHR21139. 1 hit.
    PfamiPF00121. TIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF51351. SSF51351. 1 hit.
    PROSITEiPS00171. TIM_1. 1 hit.
    PS51440. TIM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56076-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTKIAMANFK SAMPIFKSHA YLKELEKTLK PQHFDRVFVF PDFFGLLPNS
    60 70 80 90 100
    FLHFTLGVQN AYPRDCGAFT GEITSKHLEE LKIHTLLIGH SERRTLLKES
    110 120 130 140 150
    PSFLKEKFDF FKSKNFKIVY CIGEELTTRE KGFKAVKEFL SEQLENIDLN
    160 170 180 190 200
    YPNLVVAYEP IWAIGTKKSA SLEDIYLTHG FLKQILNQKT PLLYGGSVNT
    210 220 230
    QNAKEILGID SVDGLLIGSA SWELENFKTI ISFL
    Length:234
    Mass (Da):26,708
    Last modified:November 1, 1997 - v1
    Checksum:iAFEB4FF92D5BC4D3
    GO

    Mass spectrometryi

    Molecular mass is 28108 Da from positions 1 - 234. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07261.1.
    PIRiB64544.
    RefSeqiNP_206993.1. NC_000915.1.
    WP_000160988.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07261; AAD07261; HP_0194.
    GeneIDi899983.
    KEGGiheo:C694_00965.
    hpy:HP0194.
    PATRICi20591609. VBIHelPyl33062_0204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07261.1.
    PIRiB64544.
    RefSeqiNP_206993.1. NC_000915.1.
    WP_000160988.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2JGQX-ray2.30A/B2-234[»]
    ProteinModelPortaliP56076.
    SMRiP56076.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-3273N.
    IntActiP56076. 5 interactors.
    MINTiMINT-166065.
    STRINGi85962.HP0194.

    Proteomic databases

    PaxDbiP56076.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD07261; AAD07261; HP_0194.
    GeneIDi899983.
    KEGGiheo:C694_00965.
    hpy:HP0194.
    PATRICi20591609. VBIHelPyl33062_0204.

    Phylogenomic databases

    eggNOGiENOG4105CP7. Bacteria.
    COG0149. LUCA.
    KOiK01803.
    OMAiPNSFLHF.

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00189.
    UPA00138.
    BioCyciHPY:HP0194-MONOMER.
    BRENDAi5.3.1.1. 2604.
    SABIO-RKP56076.

    Miscellaneous databases

    EvolutionaryTraceiP56076.

    Family and domain databases

    CDDicd00311. TIM. 1 hit.
    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00147_B. TIM_B. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR022896. TrioseP_Isoase_bac/euk.
    IPR000652. Triosephosphate_isomerase.
    IPR020861. Triosephosphate_isomerase_AS.
    [Graphical view]
    PANTHERiPTHR21139. PTHR21139. 1 hit.
    PfamiPF00121. TIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF51351. SSF51351. 1 hit.
    PROSITEiPS00171. TIM_1. 1 hit.
    PS51440. TIM_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTPIS_HELPY
    AccessioniPrimary (citable) accession number: P56076
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: November 2, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.