Reviewed, UniProtKB/Swiss-Prot P56076 (TPIS_HELPY)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Synonyms:	tpi
Ordered Locus Names:	HP_0194

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 234

234

Triosephosphate isomerase HAMAP MF_00147

PRO_0000090228

Sites

Active site

90

1

Electrophile By similarity

Active site

159

1

Proton acceptor By similarity

Binding site

8

1

Substrate By similarity

Binding site

10

1

Substrate By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

234

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

15 – 28

14

Helix

31 – 33

3

Turn

34 – 36

3

Beta strand

37 – 40

4

Turn

43 – 45

3

Beta strand

52 – 56

5

Beta strand

62 – 68

7

Helix

75 – 80

6

Beta strand

85 – 88

4

Helix

91 – 95

5

Helix

101 – 113

13

Beta strand

117 – 122

6

Helix

126 – 131

6

Helix

133 – 144

12

Beta strand

154 – 158

5

Helix

161 – 163

3

Helix

172 – 185

14

Beta strand

192 – 197

6

Turn

200 – 202

3

Helix

203 – 207

5

Beta strand

214 – 218

5

Helix

219 – 222

4

Helix

224 – 231

8

Sequences

	Sequence		Length	Mass (Da)
	P56076-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AFEB4FF92D5BC4D3	FASTA	234	26,708
	10 20 30 40 50 60 MTKIAMANFK SAMPIFKSHA YLKELEKTLK PQHFDRVFVF PDFFGLLPNS FLHFTLGVQN 70 80 90 100 110 120 AYPRDCGAFT GEITSKHLEE LKIHTLLIGH SERRTLLKES PSFLKEKFDF FKSKNFKIVY 130 140 150 160 170 180 CIGEELTTRE KGFKAVKEFL SEQLENIDLN YPNLVVAYEP IWAIGTKKSA SLEDIYLTHG 190 200 210 220 230 FLKQILNQKT PLLYGGSVNT QNAKEILGID SVDGLLIGSA SWELENFKTI ISFL

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000511 Genomic DNA. Translation: AAD07261.1.

PIR

B64544.

RefSeq

NP_206993.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JGQ	X-ray	2.30	A/B	2-234	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:3273N.

Genome annotation databases

GeneID

899983.

GenomeReviews

Gene locus HP_0194 in contig AE000511_GR.

KEGG

hpy:HP0194.

NMPDR

fig|85962.1.peg.191.

TIGR

HP_0194.

Phylogenomic databases

HOGENOM

P56076.

OMA

DIRSVQT

Enzyme and pathway databases

BRENDA

5.3.1.1. 1131.

Family and domain databases

HAMAP

MF_00147.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR21139. Triophos_ismrse. 1 hit.

Pfam

PF00121. TIM. 1 hit.
[Graphical view]

PROSITE

PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPIS_HELPY

Accession

Primary (citable) accession number: P56076

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)