Reviewed, UniProtKB/Swiss-Prot P56074 (HEM2_HELPY)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	HP_0163

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Zinc By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	porphobilinogen synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Delta-aminolevulinic acid dehydratase		PRO_0000140502
Regions
	Region	115 – 132	18	Zinc-binding By similarity
Sites
	Active site	246	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56074-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: ADB784A450856CA2	FASTA	323	36,203
	10 20 30 40 50 60 MFKRLRRLRS SENLRAMLRE TRLNIDDFIA PLFVIESDSC IKNEISSMPG VYQMSMEPLL 70 80 90 100 110 120 KECEELVGLG VKAVLLFGIP KHKDATGSHA LNKDHIVAKA AKEIKKRFKD LIVIADLCFC 130 140 150 160 170 180 EYTDHGHCGI LENASVSNDK TLEILNLQGL ILAESGVDIL APSNMMDGNV LSLRKTLDNA 190 200 210 220 230 240 GYTHTPIMSY STKFASSYYG PFRDVANSAP SFGDRKSYQM DYANQKEALL ESLEDEKQGA 250 260 270 280 290 300 DILMVKPALA YLDIVKEIRD HTLLPLALYN VSGEYAMLKL AQKHNLINYE SVLLETMTCF 310 320 KRAGADMIIS YHAKEVANLL QRN

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07232.1.
PIR	C64540.
RefSeq	NP_206962.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	899142.
GenomeReviews	Gene locus HP_0163 in contig AE000511_GR.
KEGG	hpy:HP0163.
NMPDR	fig|85962.1.peg.160.
TIGR	HP_0163.
Phylogenomic databases
HOGENOM	P56074.
OMA	ITYFAKE
Enzyme and pathway databases
BRENDA	4.2.1.24. 1131.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_HELPY

Accession

Primary (citable) accession number: P56074

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)