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P56073 (AROK_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71
Gene names
Name:aroK
Ordered Locus Names:HP_0157
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length162 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. Ref.3

Catalytic activity

ATP + shikimate = ADP + shikimate 3-phosphate. Ref.3

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP-Rule MF_00109

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the shikimate kinase family.

Biophysicochemical properties

Kinetic parameters:

KM=60 µM for shikimate Ref.3

KM=101 µM for MgATP

Vmax=22 µmol/min/mg enzyme toward shikimate

Vmax=26 µmol/min/mg enzyme toward MgATP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 162162Shikimate kinase HAMAP-Rule MF_00109
PRO_0000192387

Regions

Nucleotide binding11 – 166ATP By similarity
Region109 – 12315LID domain HAMAP-Rule MF_00109

Sites

Metal binding151Magnesium By similarity
Binding site331Substrate
Binding site571Substrate
Binding site801Substrate; via amide nitrogen
Binding site1161ATP By similarity
Binding site1321Substrate

Secondary structure

1 ................................. 162
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56073 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 3D4B5F74E1C958FE

FASTA16218,411
        10         20         30         40         50         60 
MQHLVLIGFM GSGKSSLAQE LGLALKLEVL DTDMIISERV GLSVREIFEE LGEDNFRMFE 

        70         80         90        100        110        120 
KNLIDELKTL KTPHVISTGG GIVMHENLKG LGTTFYLKMD FETLIKRLNQ KEREKRPLLN 

       130        140        150        160 
NLTQAKELFE KRQALYEKNA SFIIDARGGL NNSLKQVLQF IA

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. expand/collapse author list , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.
Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.
[2]"Discovery of Helicobacter pylori shikimate kinase inhibitors: bioassay and molecular modeling."
Han C., Zhang J., Chen L., Chen K., Shen X., Jiang H.
Bioorg. Med. Chem. 15:656-662(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INHIBITORS.
[3]"Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase."
Cheng W.-C., Chang Y.-N., Wang W.-C.
J. Bacteriol. 187:8156-8163(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF APOENYZME AND IN COMPLEX WITH SHIKIMATE, FUNCTION, CATALYTIC ACTIVITY, KINETIC PARAMETERS.
Strain: ATCC 700392 / 26695.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000511 Genomic DNA. Translation: AAD07220.1.
PIRE64539.
RefSeqNP_206956.1. NC_000915.1.
YP_006934081.1. NC_018939.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUHX-ray1.80A1-162[»]
1ZUIX-ray2.30A1-162[»]
3HR7X-ray1.80A/B1-162[»]
3MRSX-ray2.40A1-162[»]
3MUFX-ray2.30A1-162[»]
3N2EX-ray2.53A/B/C1-162[»]
ProteinModelPortalP56073.
SMRP56073. Positions 2-161.
ModBaseSearch...

Protein-protein interaction databases

STRING85962.HP0157.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07220; AAD07220; HP_0157.
GeneID13869337.
898985.
KEGGheo:C694_00785.
hpy:HP0157.
PATRIC20591535. VBIHelPyl33062_0167.

Phylogenomic databases

eggNOGCOG0703.
KOK00891.
OMANNLTQAK.
ProtClustDBPRK00131.

Enzyme and pathway databases

SABIO-RKP56073.
UniPathwayUPA00053; UER00088.

Family and domain databases

HAMAPMF_00109. Shikimate_kinase.
InterProIPR000623. Shikimate_kinase.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamPF01202. SKI. 1 hit.
[Graphical view]
PRINTSPR01100. SHIKIMTKNASE.
PROSITEPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL1075089.
EvolutionaryTraceP56073.

Entry information

Entry nameAROK_HELPY
AccessionPrimary (citable) accession number: P56073
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents