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Protein

Shikimate kinase

Gene

aroK

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.1 Publication

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Kineticsi

  1. KM=60 µM for shikimate1 Publication
  2. KM=101 µM for MgATP1 Publication
  1. Vmax=22 µmol/min/mg enzyme toward shikimate1 Publication
  2. Vmax=26 µmol/min/mg enzyme toward MgATP1 Publication

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151MagnesiumBy similarity
Binding sitei33 – 331Substrate
Binding sitei57 – 571Substrate
Binding sitei80 – 801Substrate; via amide nitrogen
Binding sitei116 – 1161ATPBy similarity
Binding sitei132 – 1321Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP0157-MONOMER.
BRENDAi2.7.1.71. 2604.
SABIO-RKP56073.
UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinase (EC:2.7.1.71)
Short name:
SK
Gene namesi
Name:aroK
Ordered Locus Names:HP_0157
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075089.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162Shikimate kinasePRO_0000192387Add
BLAST

Proteomic databases

PaxDbiP56073.
PRIDEiP56073.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi85962.HP0157.

Chemistry

BindingDBiP56073.

Structurei

Secondary structure

1
162
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi14 – 2512Combined sources
Beta strandi29 – 313Combined sources
Helixi32 – 409Combined sources
Helixi44 – 507Combined sources
Helixi53 – 6816Combined sources
Beta strandi75 – 773Combined sources
Helixi80 – 845Combined sources
Helixi86 – 883Combined sources
Beta strandi91 – 988Combined sources
Helixi101 – 1088Combined sources
Helixi110 – 1145Combined sources
Helixi117 – 1204Combined sources
Helixi123 – 13816Combined sources
Beta strandi141 – 1455Combined sources
Helixi146 – 1483Combined sources
Helixi150 – 15910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUHX-ray1.80A1-162[»]
1ZUIX-ray2.30A1-162[»]
3HR7X-ray1.80A/B1-162[»]
3MRSX-ray2.40A1-162[»]
3MUFX-ray2.30A1-162[»]
3N2EX-ray2.53A/B/C1-162[»]
ProteinModelPortaliP56073.
SMRiP56073. Positions 2-161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56073.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 12315LID domainAdd
BLAST

Sequence similaritiesi

Belongs to the shikimate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
KOiK00891.
OMAiLKTPHII.
OrthoDBiEOG6SJJGD.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHLVLIGFM GSGKSSLAQE LGLALKLEVL DTDMIISERV GLSVREIFEE
60 70 80 90 100
LGEDNFRMFE KNLIDELKTL KTPHVISTGG GIVMHENLKG LGTTFYLKMD
110 120 130 140 150
FETLIKRLNQ KEREKRPLLN NLTQAKELFE KRQALYEKNA SFIIDARGGL
160
NNSLKQVLQF IA
Length:162
Mass (Da):18,411
Last modified:November 1, 1997 - v1
Checksum:i3D4B5F74E1C958FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07220.1.
PIRiE64539.
RefSeqiNP_206956.1. NC_000915.1.
WP_001164290.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07220; AAD07220; HP_0157.
GeneIDi898985.
KEGGiheo:C694_00785.
hpy:HP0157.
PATRICi20591535. VBIHelPyl33062_0167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07220.1.
PIRiE64539.
RefSeqiNP_206956.1. NC_000915.1.
WP_001164290.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUHX-ray1.80A1-162[»]
1ZUIX-ray2.30A1-162[»]
3HR7X-ray1.80A/B1-162[»]
3MRSX-ray2.40A1-162[»]
3MUFX-ray2.30A1-162[»]
3N2EX-ray2.53A/B/C1-162[»]
ProteinModelPortaliP56073.
SMRiP56073. Positions 2-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0157.

Chemistry

BindingDBiP56073.
ChEMBLiCHEMBL1075089.

Proteomic databases

PaxDbiP56073.
PRIDEiP56073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07220; AAD07220; HP_0157.
GeneIDi898985.
KEGGiheo:C694_00785.
hpy:HP0157.
PATRICi20591535. VBIHelPyl33062_0167.

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
KOiK00891.
OMAiLKTPHII.
OrthoDBiEOG6SJJGD.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.
BioCyciHPY:HP0157-MONOMER.
BRENDAi2.7.1.71. 2604.
SABIO-RKP56073.

Miscellaneous databases

EvolutionaryTraceiP56073.
PROiP56073.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "Discovery of Helicobacter pylori shikimate kinase inhibitors: bioassay and molecular modeling."
    Han C., Zhang J., Chen L., Chen K., Shen X., Jiang H.
    Bioorg. Med. Chem. 15:656-662(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITORS.
  3. "Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase."
    Cheng W.-C., Chang Y.-N., Wang W.-C.
    J. Bacteriol. 187:8156-8163(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF APOENYZME AND IN COMPLEX WITH SHIKIMATE, FUNCTION, CATALYTIC ACTIVITY, KINETIC PARAMETERS.
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiAROK_HELPY
AccessioniPrimary (citable) accession number: P56073
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 11, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.