Reviewed, UniProtKB/Swiss-Prot P56070 (PPSA_HELPY)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate synthase
      Short name=PEP synthase
    EC=2.7.9.2
Alternative name(s):
    Pyruvate, water dikinase

Gene names

Name:	ppsA
Ordered Locus Names:	HP_0121

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 812	812	Phosphoenolpyruvate synthase		PRO_0000147036
Sites
	Active site	430	1	Tele-phosphohistidine intermediate By similarity
	Active site	761	1	Proton donor By similarity
	Metal binding	690	1	Magnesium By similarity
	Metal binding	714	1	Magnesium By similarity
	Binding site	520	1	Substrate By similarity
	Binding site	588	1	Substrate By similarity
	Binding site	690	1	Substrate By similarity
	Binding site	711	1	Substrate; via carbonyl oxygen By similarity
	Binding site	712	1	Substrate; via amide nitrogen By similarity
	Binding site	713	1	Substrate By similarity
	Binding site	714	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56070-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 56D660703B134AA3	FASTA	812	91,273
	10 20 30 40 50 60 MRYIKFFKEL NNKNVNLVGG KNASIGEMFQ ELVPIGIKVP DGFAITSEAY WYLLEQGGAK 70 80 90 100 110 120 QKIIELLENV DATEIDVLKI RSKQIRELIF GTPFPSDLRD EIFQAYEILS QQYHMKEADV 130 140 150 160 170 180 AVRSSATAED LPDASFAGQQ DTYLNIKGKT ELIHYIKSCL ASLFTDRAIS YRASRGFDHL 190 200 210 220 230 240 KVALSVGVQK MVRADKGSAG VMFSIDTETG FKDAVFITSA WGLGENVVGG TINPDEFYVF 250 260 270 280 290 300 KPTLEQNKRP IIKRQLGNKT QKMVYAPRGS EHPTRNIKTT KKEWQSFSLS DEDVLILAKY 310 320 330 340 350 360 AIEIEKHYSK EAKQYRPMDI EWAKDGESGE IFIVQARPET VQSQKSKEES QVFEKFKFKN 370 380 390 400 410 420 PNEKKEIILQ GRAIGSKIGS GKVRIINDLE HMNSFKEGEI LVTDNTDPDW EPCMKKASAV 430 440 450 460 470 480 ITNRGGRTCH AAIVAREIGV PAIVGVSGAT DSLYTGMEIT VSCAEGEEGY VYAGIYEHEI 490 500 510 520 530 540 ERVELSNMQE TQTKIYINIG NPEKAFGFSQ LPNHGVGLAR MEMIILNQIK AHPLALVDLH 550 560 570 580 590 600 HKKSVKEKNE IENLMAGYAN PKDFFVKKIA EGIGMISAAF YPKPVIVRTS DFKSNEYMRM 610 620 630 640 650 660 LGGSSYEPNE ENPMLGYRGA SRYYSESYNE AFSWECEALA LVREEMGLTN MKVMIPFLRT 670 680 690 700 710 720 IEEGKKVLEI LRKNNLESGK NGLEIYIMCE LPVNVILADD FLSLFDGFSI GSNDLTQLTL 730 740 750 760 770 780 GVDRDSELVS HVFDERNEAM LKMFKKAIEA CKRHNKYCGI CGQAPSDYPE VTEFLVKEGI 790 800 810 TSISLNPDSV IPTWNAVAKL EKELKEHGLT EH

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07191.1.
PIR	A64535.
RefSeq	NP_206921.1.
3D structure databases
SMR	P56070. Positions 2-799.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3685N.
Genome annotation databases
GeneID	900337.
GenomeReviews	Gene locus HP_0121 in contig AE000511_GR.
KEGG	hpy:HP0121.
NMPDR	fig|85962.1.peg.119.
TIGR	HP_0121.
Phylogenomic databases
HOGENOM	HBG284863.
OMA	NKYVGIC.
Enzyme and pathway databases
BRENDA	2.7.9.2. 1131.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR008279. PEP-utiliz_enz_mobile_dom. IPR018274. PEP_mobile_CS. IPR006318. PEP_P_trans. IPR006319. PEP_synth. IPR000121. PEP_utilisers. IPR002192. PPDK_PEP_bd. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
Pfam	PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
PRINTS	PR01736. PHPHTRNFRASE.
TIGRFAMs	TIGR01418. PEP_synth. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_HELPY

Accession

Primary (citable) accession number: P56070

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)