Reviewed, UniProtKB/Swiss-Prot P56069 (METB_HELPY)

Names and origin

Protein names

Recommended name:
    Cystathionine gamma-synthase
      Short name=CGS
    EC=2.5.1.48
Alternative name(s):
    O-succinylhomoserine (thiol)-lyase

Gene names

Name:	metB
Ordered Locus Names:	HP_0106

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Cystathionine gamma-synthase		PRO_0000114758
Amino acid modifications
	Modified residue	195	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56069-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 58A85489B05257ED	FASTA	380	41,118
	10 20 30 40 50 60 MRMQTKLIHG GISEDATTGA VSVPIYQTST YRQDAIGRHK GYEYSRSGNP TRFALEELIA 70 80 90 100 110 120 DLEGGVKGFA FASGLAGIHA VFSLLQSGDH VLLGDDVYGG TFRLFNQVLV KNGLSCTIID 130 140 150 160 170 180 TSDISQIKKA IKPNTKALYL ETPSNPLLKI TDLAQCASVA KDHGLLTIVD NTFATPYYQN 190 200 210 220 230 240 PLLLGADIVA HSGTKYLGGH SDVVAGLVTT NNEALAQEIA FFQNAIGGVL GPQDSWLLQR 250 260 270 280 290 300 GIKTLGLRME AHQKNALCVA EFLEKHPKVE RVYYPGLPTH PNYELAKKQM RGFSGMLSFT 310 320 330 340 350 360 LKNDSEAVAF VESLKLFILG ESLGGVESLV GIPAFMTHAC IPKTQREAAG IRDGLVRLSV 370 380 GIEHEQDLLE DLEQAFAKIG

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07176.1.
PIR	B64533.
RefSeq	NP_206906.1.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
ModBase	Search...
Genome annotation databases
GeneID	898970.
GenomeReviews	Gene locus HP_0106 in contig AE000511_GR.
KEGG	hpy:HP0106.
NMPDR	fig|85962.1.peg.104.
TIGR	HP_0106.
Phylogenomic databases
HOGENOM	P56069.
OMA	NQYEYSR.
Enzyme and pathway databases
BRENDA	2.5.1.48. 1131.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_HELPY

Accession

Primary (citable) accession number: P56069

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)