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Protein

Cystathionine gamma-synthase

Gene

metB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

pyridoxal 5'-phosphateBy similarityNote: Binds 1 pyridoxal phosphate per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciHPY:HP0106-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase (EC:2.5.1.48)
Short name:
CGS
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Name:metB
Ordered Locus Names:HP_0106
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147581 – 380Cystathionine gamma-synthaseAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP56069.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP56069. 1 interactor.
STRINGi85962.HP0106.

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 9Combined sources7
Beta strandi10 – 12Combined sources3
Turni16 – 18Combined sources3
Turni45 – 47Combined sources3
Helixi50 – 63Combined sources14
Beta strandi66 – 73Combined sources8
Helixi74 – 82Combined sources9
Beta strandi90 – 94Combined sources5
Helixi99 – 107Combined sources9
Helixi110 – 112Combined sources3
Beta strandi115 – 119Combined sources5
Helixi124 – 130Combined sources7
Beta strandi135 – 143Combined sources9
Turni145 – 147Combined sources3
Helixi153 – 161Combined sources9
Turni162 – 164Combined sources3
Beta strandi166 – 170Combined sources5
Beta strandi172 – 174Combined sources3
Turni176 – 178Combined sources3
Helixi181 – 184Combined sources4
Beta strandi187 – 192Combined sources6
Turni193 – 198Combined sources6
Beta strandi206 – 211Combined sources6
Helixi213 – 226Combined sources14
Helixi232 – 242Combined sources11
Helixi245 – 264Combined sources20
Beta strandi267 – 273Combined sources7
Helixi283 – 289Combined sources7
Beta strandi295 – 303Combined sources9
Helixi306 – 312Combined sources7
Beta strandi315 – 319Combined sources5
Beta strandi324 – 327Combined sources4
Beta strandi329 – 331Combined sources3
Turni333 – 340Combined sources8
Helixi343 – 348Combined sources6
Beta strandi355 – 359Combined sources5
Helixi365 – 377Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4L0OX-ray2.76A/C/E/G/H/K/M/O1-380[»]
ProteinModelPortaliP56069.
SMRiP56069.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
KOiK01760.
OMAiTHACIPK.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMQTKLIHG GISEDATTGA VSVPIYQTST YRQDAIGRHK GYEYSRSGNP
60 70 80 90 100
TRFALEELIA DLEGGVKGFA FASGLAGIHA VFSLLQSGDH VLLGDDVYGG
110 120 130 140 150
TFRLFNQVLV KNGLSCTIID TSDISQIKKA IKPNTKALYL ETPSNPLLKI
160 170 180 190 200
TDLAQCASVA KDHGLLTIVD NTFATPYYQN PLLLGADIVA HSGTKYLGGH
210 220 230 240 250
SDVVAGLVTT NNEALAQEIA FFQNAIGGVL GPQDSWLLQR GIKTLGLRME
260 270 280 290 300
AHQKNALCVA EFLEKHPKVE RVYYPGLPTH PNYELAKKQM RGFSGMLSFT
310 320 330 340 350
LKNDSEAVAF VESLKLFILG ESLGGVESLV GIPAFMTHAC IPKTQREAAG
360 370 380
IRDGLVRLSV GIEHEQDLLE DLEQAFAKIG
Length:380
Mass (Da):41,118
Last modified:November 1, 1997 - v1
Checksum:i58A85489B05257ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07176.1.
PIRiB64533.
RefSeqiNP_206906.1. NC_000915.1.
WP_001242837.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07176; AAD07176; HP_0106.
GeneIDi898970.
KEGGiheo:C694_00525.
hpy:HP0106.
PATRICi20591427. VBIHelPyl33062_0113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07176.1.
PIRiB64533.
RefSeqiNP_206906.1. NC_000915.1.
WP_001242837.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4L0OX-ray2.76A/C/E/G/H/K/M/O1-380[»]
ProteinModelPortaliP56069.
SMRiP56069.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56069. 1 interactor.
STRINGi85962.HP0106.

Proteomic databases

PaxDbiP56069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07176; AAD07176; HP_0106.
GeneIDi898970.
KEGGiheo:C694_00525.
hpy:HP0106.
PATRICi20591427. VBIHelPyl33062_0113.

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
KOiK01760.
OMAiTHACIPK.

Enzyme and pathway databases

BioCyciHPY:HP0106-MONOMER.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETB_HELPY
AccessioniPrimary (citable) accession number: P56069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.