Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cystathionine gamma-synthase

Gene

metB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

pyridoxal 5'-phosphateBy similarityNote: Binds 1 pyridoxal phosphate per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciHPY:HP0106-MONOMER.
RETL1328306-WGS:GSTH-4654-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase (EC:2.5.1.48)
Short name:
CGS
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Name:metB
Ordered Locus Names:HP_0106
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Cystathionine gamma-synthasePRO_0000114758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei195 – 1951N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiP56069.
PRIDEiP56069.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP56069. 1 interaction.
STRINGi85962.HP0106.

Structurei

Secondary structure

1
380
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97Combined sources
Beta strandi10 – 123Combined sources
Turni16 – 183Combined sources
Turni45 – 473Combined sources
Helixi50 – 6314Combined sources
Beta strandi66 – 738Combined sources
Helixi74 – 829Combined sources
Beta strandi90 – 945Combined sources
Helixi99 – 1079Combined sources
Helixi110 – 1123Combined sources
Beta strandi115 – 1195Combined sources
Helixi124 – 1307Combined sources
Beta strandi135 – 1439Combined sources
Turni145 – 1473Combined sources
Helixi153 – 1619Combined sources
Turni162 – 1643Combined sources
Beta strandi166 – 1705Combined sources
Beta strandi172 – 1743Combined sources
Turni176 – 1783Combined sources
Helixi181 – 1844Combined sources
Beta strandi187 – 1926Combined sources
Turni193 – 1986Combined sources
Beta strandi206 – 2116Combined sources
Helixi213 – 22614Combined sources
Helixi232 – 24211Combined sources
Helixi245 – 26420Combined sources
Beta strandi267 – 2737Combined sources
Helixi283 – 2897Combined sources
Beta strandi295 – 3039Combined sources
Helixi306 – 3127Combined sources
Beta strandi315 – 3195Combined sources
Beta strandi324 – 3274Combined sources
Beta strandi329 – 3313Combined sources
Turni333 – 3408Combined sources
Helixi343 – 3486Combined sources
Beta strandi355 – 3595Combined sources
Helixi365 – 37713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L0OX-ray2.76A/C/E/G/H/K/M/O1-380[»]
ProteinModelPortaliP56069.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
KOiK01760.
OMAiTHACIPK.
OrthoDBiEOG67DPN3.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMQTKLIHG GISEDATTGA VSVPIYQTST YRQDAIGRHK GYEYSRSGNP
60 70 80 90 100
TRFALEELIA DLEGGVKGFA FASGLAGIHA VFSLLQSGDH VLLGDDVYGG
110 120 130 140 150
TFRLFNQVLV KNGLSCTIID TSDISQIKKA IKPNTKALYL ETPSNPLLKI
160 170 180 190 200
TDLAQCASVA KDHGLLTIVD NTFATPYYQN PLLLGADIVA HSGTKYLGGH
210 220 230 240 250
SDVVAGLVTT NNEALAQEIA FFQNAIGGVL GPQDSWLLQR GIKTLGLRME
260 270 280 290 300
AHQKNALCVA EFLEKHPKVE RVYYPGLPTH PNYELAKKQM RGFSGMLSFT
310 320 330 340 350
LKNDSEAVAF VESLKLFILG ESLGGVESLV GIPAFMTHAC IPKTQREAAG
360 370 380
IRDGLVRLSV GIEHEQDLLE DLEQAFAKIG
Length:380
Mass (Da):41,118
Last modified:November 1, 1997 - v1
Checksum:i58A85489B05257ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07176.1.
PIRiB64533.
RefSeqiNP_206906.1. NC_000915.1.
WP_001242837.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07176; AAD07176; HP_0106.
GeneIDi898970.
KEGGiheo:C694_00525.
hpy:HP0106.
PATRICi20591427. VBIHelPyl33062_0113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07176.1.
PIRiB64533.
RefSeqiNP_206906.1. NC_000915.1.
WP_001242837.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L0OX-ray2.76A/C/E/G/H/K/M/O1-380[»]
ProteinModelPortaliP56069.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56069. 1 interaction.
STRINGi85962.HP0106.

Proteomic databases

PaxDbiP56069.
PRIDEiP56069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07176; AAD07176; HP_0106.
GeneIDi898970.
KEGGiheo:C694_00525.
hpy:HP0106.
PATRICi20591427. VBIHelPyl33062_0113.

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
KOiK01760.
OMAiTHACIPK.
OrthoDBiEOG67DPN3.

Enzyme and pathway databases

BioCyciHPY:HP0106-MONOMER.
RETL1328306-WGS:GSTH-4654-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiMETB_HELPY
AccessioniPrimary (citable) accession number: P56069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 11, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.