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Protein

Cysteine synthase

Gene

cysM

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.

Cofactori

Pathway: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Cysteine synthase (cysM)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761Pyridoxal phosphateBy similarity
Binding sitei267 – 2671Pyridoxal phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciHPY:HP0107-MONOMER.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase (EC:2.5.1.47)
Short name:
CSase
Alternative name(s):
O-acetylserine (thiol)-lyase
Short name:
OAS-TL
O-acetylserine sulfhydrylase
Gene namesi
Name:cysM
Synonyms:cysK
Ordered Locus Names:HP_0107
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Cysteine synthasePRO_0000167110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PRIDEiP56067.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

DIPiDIP-3738N.
IntActiP56067. 1 interaction.
MINTiMINT-168918.
STRINGi85962.HP0107.

Structurei

Secondary structure

1
306
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Helixi7 – 104Combined sources
Beta strandi16 – 194Combined sources
Turni21 – 233Combined sources
Beta strandi31 – 366Combined sources
Helixi37 – 393Combined sources
Helixi46 – 6015Combined sources
Beta strandi68 – 725Combined sources
Helixi76 – 8510Combined sources
Turni86 – 894Combined sources
Beta strandi91 – 977Combined sources
Helixi102 – 1109Combined sources
Beta strandi114 – 1185Combined sources
Helixi123 – 13715Combined sources
Turni146 – 1483Combined sources
Helixi151 – 1588Combined sources
Helixi160 – 1689Combined sources
Beta strandi174 – 1785Combined sources
Beta strandi180 – 1823Combined sources
Helixi183 – 19513Combined sources
Beta strandi200 – 2067Combined sources
Beta strandi240 – 2445Combined sources
Helixi246 – 26015Combined sources
Helixi266 – 28116Combined sources
Beta strandi287 – 2926Combined sources
Helixi296 – 2994Combined sources
Turni300 – 3056Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R2VX-ray1.90A/B1-306[»]
ProteinModelPortaliP56067.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 1845Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0031.
KOiK01738.
OMAiMSKEIFN.
OrthoDBiEOG6Q2SP8.

Family and domain databases

InterProiIPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMIITTMQDA IGRTPVFKFT NKDYPIPLNS AIYAKLEHLN PGGSVKDRLG
60 70 80 90 100
QYLIGEGFKT GKITSKTTII EPTAGNTGIA LALVAIKHHL KTIFVVPEKF
110 120 130 140 150
STEKQQIMRA LGALVINTPT SEGISGAIKK SKELAESIPD SYLPLQFENP
160 170 180 190 200
DNPAAYYHTL APEIVQELGT NLTSFVAGIG SGGTFAGTAR YLKERIPAIR
210 220 230 240 250
LIGVEPEGSI LNGGEPGPHE IEGIGVEFIP PFFENLDIDG FETISDEEGF
260 270 280 290 300
SYTRKLAKKN GLLVGSSSGA AFVAALKEAQ RLPEGSQVLT IFPDVADRYL

SKGIYL
Length:306
Mass (Da):32,942
Last modified:November 1, 1997 - v1
Checksum:iAE2FF7EE0373DA17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07177.1.
PIRiC64533.
RefSeqiNP_206907.1. NC_000915.1.
WP_000972731.1. NC_000915.1.

Genome annotation databases

EnsemblBacteriaiAAD07177; AAD07177; HP_0107.
GeneIDi899978.
KEGGihpy:HP0107.
PATRICi20591429. VBIHelPyl33062_0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07177.1.
PIRiC64533.
RefSeqiNP_206907.1. NC_000915.1.
WP_000972731.1. NC_000915.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R2VX-ray1.90A/B1-306[»]
ProteinModelPortaliP56067.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3738N.
IntActiP56067. 1 interaction.
MINTiMINT-168918.
STRINGi85962.HP0107.

Proteomic databases

PRIDEiP56067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07177; AAD07177; HP_0107.
GeneIDi899978.
KEGGihpy:HP0107.
PATRICi20591429. VBIHelPyl33062_0114.

Phylogenomic databases

eggNOGiCOG0031.
KOiK01738.
OMAiMSKEIFN.
OrthoDBiEOG6Q2SP8.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BioCyciHPY:HP0107-MONOMER.

Family and domain databases

InterProiIPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiCYSM_HELPY
AccessioniPrimary (citable) accession number: P56067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.