P56067 (CYSM_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	cysM
Synonyms:	cysK
Ordered Locus Names:	HP_0107

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine synthase activity Inferred from electronic annotation. Source: EC transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Cysteine synthase		PRO_0000167110
Regions
	Region	180 – 184	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	76	1	Pyridoxal phosphate By similarity
	Binding site	267	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56067 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AE2FF7EE0373DA17	FASTA	306	32,942
	10 20 30 40 50 60 MMIITTMQDA IGRTPVFKFT NKDYPIPLNS AIYAKLEHLN PGGSVKDRLG QYLIGEGFKT 70 80 90 100 110 120 GKITSKTTII EPTAGNTGIA LALVAIKHHL KTIFVVPEKF STEKQQIMRA LGALVINTPT 130 140 150 160 170 180 SEGISGAIKK SKELAESIPD SYLPLQFENP DNPAAYYHTL APEIVQELGT NLTSFVAGIG 190 200 210 220 230 240 SGGTFAGTAR YLKERIPAIR LIGVEPEGSI LNGGEPGPHE IEGIGVEFIP PFFENLDIDG 250 260 270 280 290 300 FETISDEEGF SYTRKLAKKN GLLVGSSSGA AFVAALKEAQ RLPEGSQVLT IFPDVADRYL SKGIYL

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07177.1.
PIR	C64533.
RefSeq	NP_206907.1. NC_000915.1.
3D structure databases
ProteinModelPortal	P56067.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3738N.
MINT	MINT-168918.
STRING	85962.HP0107.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07177; AAD07177; HP_0107.
GeneID	899978.
KEGG	hpy:HP0107.
PATRIC	20591429. VBIHelPyl33062_0114.
Phylogenomic databases
eggNOG	COG0031.
KO	K01738.
OMA	VQELGTN.
ProtClustDB	CLSK495864.
Enzyme and pathway databases
UniPathway	UPA00136; UER00200.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_HELPY

Accession

Primary (citable) accession number: P56067

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program