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P56064 (DNAG_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA primase

EC=2.7.7.-
Gene names
Name:dnaG
Ordered Locus Names:HP_0012
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication By similarity. HAMAP-Rule MF_00974

Cofactor

Binds 1 zinc ion per monomer By similarity. HAMAP-Rule MF_00974

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00974

Subunit structure

Monomer By similarity. Interacts with DnaB By similarity. HAMAP-Rule MF_00974

Domain

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain By similarity. HAMAP-Rule MF_00974

Sequence similarities

Belongs to the DnaG primase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559DNA primase HAMAP-Rule MF_00974
PRO_0000180495

Regions

Domain246 – 32782Toprim
Zinc finger37 – 6125CHC2-type By similarity

Sites

Metal binding2521Magnesium 1; catalytic By similarity
Metal binding2961Magnesium 1; catalytic By similarity
Metal binding2961Magnesium 2 By similarity
Metal binding2981Magnesium 2 By similarity

Secondary structure

................ 559
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56064 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: E5EB7F2F0BB38170

FASTA55963,723
        10         20         30         40         50         60 
MILKSSIDRL LQTIDIVEVI SSYVNLRKSG SSYMACCPFH EERSASFSVN QIKGFYHCFG 

        70         80         90        100        110        120 
CGASGDSIKF VMAFEKLSFV EALEKLAHRF NIVLEYDKGV YYDHKEDYHL LEMVSSLYQE 

       130        140        150        160        170        180 
ELFNAPFFLN YLQKRGLSLE SIKAFKLGLC TNRIDYGIEN KGLNKDKLIE LGVLGKSDND 

       190        200        210        220        230        240 
QKTYLRFLDR IMFPIYSPSA QVVGFGGRTL KEKAAKYINS PQSKLFDKSS LLYGYHLAKE 

       250        260        270        280        290        300 
HIYKQKQVIV TEGYLDVILL HQAGFKNAIA TLGTALTPSH LPLLKKGDPE ILLSYDGDKA 

       310        320        330        340        350        360 
GRNAAYKASL MLAKEQRRGG VILFENNLDP ADMIANGQIE TLKNWLSHPM AFIEFVLRRM 

       370        380        390        400        410        420 
ADSYLLDDPL EKDKALKEML GFLKNFSLLL QSEYKPLIAT LLQAPLHVLG IRERVSFQPF 

       430        440        450        460        470        480 
YPKTEKPNRP QRFAHVSSAP SLEFLEKLVI RYLLEDRSLL DLAVGYIHSG VFLHKKQEFD 

       490        500        510        520        530        540 
ALCQEKLDDP KLVALLLDAN LPLKKGGFEK ELRLLILRYF ERQLKEIPKS SLPFSEKMIC 

       550 
LKKARQAIMK LKQGELVAI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07082.1.
PIRD64521.
RefSeqNP_206814.1. NC_000915.1.
YP_006933935.1. NC_018939.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EHSX-ray1.78A/B413-559[»]
ProteinModelPortalP56064.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3301N.
IntActP56064. 9 interactions.
MINTMINT-181460.
STRING85962.HP0012.

Proteomic databases

PRIDEP56064.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07082; AAD07082; HP_0012.
GeneID899091.
KEGGheo:C694_00055.
hpy:HP0012.
PATRIC20591217. VBIHelPyl33062_0010.

Phylogenomic databases

eggNOGCOG0358.
KOK02316.
OMAEYDKGAY.
OrthoDBEOG6XDGTR.

Enzyme and pathway databases

BioCycHPY:HP0012-MONOMER.

Family and domain databases

Gene3D3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPMF_00974. DNA_primase_DnaG.
InterProIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamPF13662. Toprim_4. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
PIRSFPIRSF002811. DnaG. 1 hit.
SMARTSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsTIGR01391. dnaG. 1 hit.
PROSITEPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAG_HELPY
AccessionPrimary (citable) accession number: P56064
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names