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P56064

- DNAG_HELPY

UniProt

P56064 - DNAG_HELPY

Protein

DNA primase

Gene

dnaG

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

    Cofactori

    Binds 1 zinc ion per monomer.UniRule annotation
    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi252 – 2521Magnesium 1; catalyticUniRule annotation
    Metal bindingi296 – 2961Magnesium 1; catalyticUniRule annotation
    Metal bindingi296 – 2961Magnesium 2UniRule annotation
    Metal bindingi298 – 2981Magnesium 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri37 – 6125CHC2-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA primase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Transcription

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciHPY:HP0012-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
    Gene namesi
    Name:dnaGUniRule annotation
    Ordered Locus Names:HP_0012
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 559559DNA primasePRO_0000180495Add
    BLAST

    Proteomic databases

    PRIDEiP56064.

    Interactioni

    Subunit structurei

    Monomer. Interacts with DnaB.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-3301N.
    IntActiP56064. 9 interactions.
    MINTiMINT-181460.
    STRINGi85962.HP0012.

    Structurei

    Secondary structure

    1
    559
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi442 – 45514
    Helixi457 – 4637
    Turni464 – 4663
    Helixi469 – 4713
    Helixi476 – 4838
    Helixi490 – 4978
    Helixi508 – 52922
    Helixi534 – 55219

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EHSX-ray1.78A/B413-559[»]
    ProteinModelPortaliP56064.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini246 – 32782ToprimUniRule annotationAdd
    BLAST

    Domaini

    Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaG primase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri37 – 6125CHC2-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0358.
    KOiK02316.
    OMAiEYDKGAY.
    OrthoDBiEOG6XDGTR.

    Family and domain databases

    Gene3Di3.90.580.10. 1 hit.
    3.90.980.10. 1 hit.
    HAMAPiMF_00974. DNA_primase_DnaG.
    InterProiIPR013264. DNA_primase_core_N.
    IPR006295. DNA_primase_DnaG.
    IPR006171. Toprim_domain.
    IPR002694. Znf_CHC2.
    [Graphical view]
    PfamiPF13662. Toprim_4. 1 hit.
    PF08275. Toprim_N. 1 hit.
    PF01807. zf-CHC2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002811. DnaG. 1 hit.
    SMARTiSM00493. TOPRIM. 1 hit.
    SM00400. ZnF_CHCC. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01391. dnaG. 1 hit.
    PROSITEiPS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56064-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILKSSIDRL LQTIDIVEVI SSYVNLRKSG SSYMACCPFH EERSASFSVN    50
    QIKGFYHCFG CGASGDSIKF VMAFEKLSFV EALEKLAHRF NIVLEYDKGV 100
    YYDHKEDYHL LEMVSSLYQE ELFNAPFFLN YLQKRGLSLE SIKAFKLGLC 150
    TNRIDYGIEN KGLNKDKLIE LGVLGKSDND QKTYLRFLDR IMFPIYSPSA 200
    QVVGFGGRTL KEKAAKYINS PQSKLFDKSS LLYGYHLAKE HIYKQKQVIV 250
    TEGYLDVILL HQAGFKNAIA TLGTALTPSH LPLLKKGDPE ILLSYDGDKA 300
    GRNAAYKASL MLAKEQRRGG VILFENNLDP ADMIANGQIE TLKNWLSHPM 350
    AFIEFVLRRM ADSYLLDDPL EKDKALKEML GFLKNFSLLL QSEYKPLIAT 400
    LLQAPLHVLG IRERVSFQPF YPKTEKPNRP QRFAHVSSAP SLEFLEKLVI 450
    RYLLEDRSLL DLAVGYIHSG VFLHKKQEFD ALCQEKLDDP KLVALLLDAN 500
    LPLKKGGFEK ELRLLILRYF ERQLKEIPKS SLPFSEKMIC LKKARQAIMK 550
    LKQGELVAI 559
    Length:559
    Mass (Da):63,723
    Last modified:November 1, 1997 - v1
    Checksum:iE5EB7F2F0BB38170
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07082.1.
    PIRiD64521.
    RefSeqiNP_206814.1. NC_000915.1.
    YP_006933935.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07082; AAD07082; HP_0012.
    GeneIDi13869189.
    899091.
    KEGGiheo:C694_00055.
    hpy:HP0012.
    PATRICi20591217. VBIHelPyl33062_0010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07082.1 .
    PIRi D64521.
    RefSeqi NP_206814.1. NC_000915.1.
    YP_006933935.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EHS X-ray 1.78 A/B 413-559 [» ]
    ProteinModelPortali P56064.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3301N.
    IntActi P56064. 9 interactions.
    MINTi MINT-181460.
    STRINGi 85962.HP0012.

    Proteomic databases

    PRIDEi P56064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07082 ; AAD07082 ; HP_0012 .
    GeneIDi 13869189.
    899091.
    KEGGi heo:C694_00055.
    hpy:HP0012.
    PATRICi 20591217. VBIHelPyl33062_0010.

    Phylogenomic databases

    eggNOGi COG0358.
    KOi K02316.
    OMAi EYDKGAY.
    OrthoDBi EOG6XDGTR.

    Enzyme and pathway databases

    BioCyci HPY:HP0012-MONOMER.

    Family and domain databases

    Gene3Di 3.90.580.10. 1 hit.
    3.90.980.10. 1 hit.
    HAMAPi MF_00974. DNA_primase_DnaG.
    InterProi IPR013264. DNA_primase_core_N.
    IPR006295. DNA_primase_DnaG.
    IPR006171. Toprim_domain.
    IPR002694. Znf_CHC2.
    [Graphical view ]
    Pfami PF13662. Toprim_4. 1 hit.
    PF08275. Toprim_N. 1 hit.
    PF01807. zf-CHC2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002811. DnaG. 1 hit.
    SMARTi SM00493. TOPRIM. 1 hit.
    SM00400. ZnF_CHCC. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01391. dnaG. 1 hit.
    PROSITEi PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    2. "Crystal structure and mode of helicase binding of the C-terminal domain of primase from Helicobacter pylori."
      Abdul Rehman S.A., Verma V., Mazumder M., Dhar S.K., Gourinath S.
      J. Bacteriol. 195:2826-2838(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 413-559.

    Entry informationi

    Entry nameiDNAG_HELPY
    AccessioniPrimary (citable) accession number: P56064
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3