P56064 (PRIM_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase
EC=2.7.7.-

Gene names

Name:	dnaG
Ordered Locus Names:	HP_0012

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA primase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	DNA primase		PRO_0000180495
Regions
	Domain	246 – 327	82	Toprim
	Zinc finger	37 – 61	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56064 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E5EB7F2F0BB38170	FASTA	559	63,723
	10 20 30 40 50 60 MILKSSIDRL LQTIDIVEVI SSYVNLRKSG SSYMACCPFH EERSASFSVN QIKGFYHCFG 70 80 90 100 110 120 CGASGDSIKF VMAFEKLSFV EALEKLAHRF NIVLEYDKGV YYDHKEDYHL LEMVSSLYQE 130 140 150 160 170 180 ELFNAPFFLN YLQKRGLSLE SIKAFKLGLC TNRIDYGIEN KGLNKDKLIE LGVLGKSDND 190 200 210 220 230 240 QKTYLRFLDR IMFPIYSPSA QVVGFGGRTL KEKAAKYINS PQSKLFDKSS LLYGYHLAKE 250 260 270 280 290 300 HIYKQKQVIV TEGYLDVILL HQAGFKNAIA TLGTALTPSH LPLLKKGDPE ILLSYDGDKA 310 320 330 340 350 360 GRNAAYKASL MLAKEQRRGG VILFENNLDP ADMIANGQIE TLKNWLSHPM AFIEFVLRRM 370 380 390 400 410 420 ADSYLLDDPL EKDKALKEML GFLKNFSLLL QSEYKPLIAT LLQAPLHVLG IRERVSFQPF 430 440 450 460 470 480 YPKTEKPNRP QRFAHVSSAP SLEFLEKLVI RYLLEDRSLL DLAVGYIHSG VFLHKKQEFD 490 500 510 520 530 540 ALCQEKLDDP KLVALLLDAN LPLKKGGFEK ELRLLILRYF ERQLKEIPKS SLPFSEKMIC 550 LKKARQAIMK LKQGELVAI

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07082.1.
PIR	D64521.
RefSeq	NP_206814.1. NC_000915.1. YP_006933935.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56064.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3301N.
MINT	MINT-181460.
STRING	85962.HP0012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07082; AAD07082; HP_0012.
GeneID	13869189. 899091.
KEGG	heo:C694_00055. hpy:HP0012.
PATRIC	20591217. VBIHelPyl33062_0010.
Phylogenomic databases
eggNOG	COG0358.
KO	K02316.
OMA	ASFQPFY.
ProtClustDB	PRK05667.
Family and domain databases
Gene3D	3.90.580.10. 1 hit. 3.90.980.10. 1 hit.
InterPro	IPR013264. DNA_primase_core_N. IPR006295. DNA_primase_DnaG. IPR006171. Toprim_domain. IPR002694. Znf_CHC2. [Graphical view]
Pfam	PF01751. Toprim. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
PIRSF	PIRSF002811. DnaG. 1 hit.
SMART	SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. dnaG. 1 hit.
PROSITE	PS50880. TOPRIM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIM_HELPY

Accession

Primary (citable) accession number: P56064

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program