P56063 (IDH_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP]
Short name=IDH
EC=1.1.1.42

Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase

Gene names

Name:	icd
Ordered Locus Names:	HP_0027

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Isocitrate dehydrogenase [NADP]		PRO_0000083552
Regions
	Nucleotide binding	348 – 354	7	NADP By similarity
Sites
	Metal binding	316	1	Magnesium or manganese By similarity
	Binding site	114	1	NADP By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	361	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	400	1	NADP By similarity
	Binding site	404	1	NADP By similarity
	Site	169	1	Critical for catalysis By similarity
	Site	239	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	123	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56063 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3741722B8818DC96	FASTA	425	47,531
	10 20 30 40 50 60 MAYNPKILQK PKEGEEITIK DNKLHVPNHP IIPFIEGDGI GSDITPAMIK VVDSAVQKAY 70 80 90 100 110 120 KGEKKIAWYE VFVGEKCYQK FKDYKELSPE EQWLLPDTIE AINHYKVSIK GPLTTPIGEG 130 140 150 160 170 180 FRSLNVALRQ KMDLYVCLRP VRWYGSPSPV KEPQKVDMVI FRENSEDIYA GIEWQEGSAE 190 200 210 220 230 240 AKKLIHFLQN ELKVKKIRFP ESSGIGVKPI SKEGTERLVR KAIEYAIDND KPSVTFVHKG 250 260 270 280 290 300 NIMKYTEGAF MKWGYALAQK EFNAQVIDKG PWCSLKNPKN GKEIIIKDMI ADAFLQQILL 310 320 330 340 350 360 RPSEYSVIAT MNLNGDYISD ALAAMVGGIG IAPGANLNDT VGMFEATHGT APKYAGLDKV 370 380 390 400 410 420 NPGSIILSAE MMLRHMGWVE AADLIVSAME KAIKSKKVTY DFARLMDGAK EVKCSEFASV MIENM

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07098.1.
PIR	C64523.
RefSeq	NP_206829.1. NC_000915.1. YP_006933949.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56063.
SMR	P56063. Positions 11-425.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3724N.
MINT	MINT-180352.
STRING	85962.HP0027.
Proteomic databases
PRIDE	P56063.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07098; AAD07098; HP_0027.
GeneID	13869203. 899116.
KEGG	heo:C694_00125. hpy:HP0027.
PATRIC	20591251. VBIHelPyl33062_0027.
Phylogenomic databases
eggNOG	COG0538.
KO	K00031.
OMA	FRDWGYE.
ProtClustDB	CLSK495824.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004439. Isocitrate_DH_NADP_dimer_prok. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11835. PTHR11835. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00183. prok_nadp_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_HELPY

Accession

Primary (citable) accession number: P56063

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program