Reviewed, UniProtKB/Swiss-Prot P56063 (IDH_HELPY)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:	icd
Ordered Locus Names:	HP_0027

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Isocitrate dehydrogenase [NADP]		PRO_0000083552
Regions
	Nucleotide binding	348 – 354	7	NADP By similarity
Sites
	Metal binding	316	1	Magnesium or manganese By similarity
	Binding site	114	1	NADP By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	361	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	400	1	NADP By similarity
	Binding site	404	1	NADP By similarity
	Site	169	1	Critical for catalysis By similarity
	Site	239	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	123	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56063-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3741722B8818DC96	FASTA	425	47,531
	10 20 30 40 50 60 MAYNPKILQK PKEGEEITIK DNKLHVPNHP IIPFIEGDGI GSDITPAMIK VVDSAVQKAY 70 80 90 100 110 120 KGEKKIAWYE VFVGEKCYQK FKDYKELSPE EQWLLPDTIE AINHYKVSIK GPLTTPIGEG 130 140 150 160 170 180 FRSLNVALRQ KMDLYVCLRP VRWYGSPSPV KEPQKVDMVI FRENSEDIYA GIEWQEGSAE 190 200 210 220 230 240 AKKLIHFLQN ELKVKKIRFP ESSGIGVKPI SKEGTERLVR KAIEYAIDND KPSVTFVHKG 250 260 270 280 290 300 NIMKYTEGAF MKWGYALAQK EFNAQVIDKG PWCSLKNPKN GKEIIIKDMI ADAFLQQILL 310 320 330 340 350 360 RPSEYSVIAT MNLNGDYISD ALAAMVGGIG IAPGANLNDT VGMFEATHGT APKYAGLDKV 370 380 390 400 410 420 NPGSIILSAE MMLRHMGWVE AADLIVSAME KAIKSKKVTY DFARLMDGAK EVKCSEFASV MIENM

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07098.1.
PIR	C64523.
RefSeq	NP_206829.1.
3D structure databases
HSSP	HSSP built from PDB template 1PB1 based on UniProtKB P08200.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3724N.
Genome annotation databases
GeneID	899116.
GenomeReviews	Gene locus HP_0027 in contig AE000511_GR.
KEGG	hpy:HP0027.
NMPDR	fig|85962.1.peg.27.
TIGR	HP_0027.
Phylogenomic databases
HOGENOM	P56063.
Family and domain databases
InterPro	IPR004439. IsoCit_DHase_NADP_prok. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF1. NADP_IDH_prok. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00183. prok_nadp_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_HELPY

Accession

Primary (citable) accession number: P56063

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)