Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P56029

- RL1_HELPY

UniProt

P56029 - RL1_HELPY

Protein

50S ribosomal protein L1

Gene

rplA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.UniRule annotation
    Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.UniRule annotation
    Peptides originating from the N-terminal end of L1 have antibacterial activity against bacteria such as E.coli and B.megaterium and modest antifungal activities. Has no effect on H.pylori itself. Peptides are not hemolytic against mammalian cells. These peptides may be released in the stomach during altruistic lysis to kill other fast growing bacteria.

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro
    3. tRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. regulation of translation Source: UniProtKB-KW
    3. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Repressor, Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Translation regulation

    Keywords - Ligandi

    RNA-binding, rRNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciHPY:HP1201-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L1UniRule annotation
    Gene namesi
    Name:rplAUniRule annotation
    Ordered Locus Names:HP_1201
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. large ribosomal subunit Source: InterPro

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi17 – 193QND → WNW: Increases antibacterial and antifungal activities 2-4 fold without an increase in hemolytic activies. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 23423450S ribosomal protein L1PRO_0000125667Add
    BLAST

    Proteomic databases

    PRIDEiP56029.

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit.

    Protein-protein interaction databases

    STRINGi85962.HP1201.

    Structurei

    Secondary structure

    1
    234
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OT0NMR-A2-18[»]
    1P0GNMR-A2-20[»]
    1P0JNMR-A2-18[»]
    1P0LNMR-A2-20[»]
    1P0ONMR-A2-20[»]
    1P5KNMR-A2-20[»]
    1P5LNMR-A7-20[»]
    ProteinModelPortaliP56029.
    SMRiP56029. Positions 7-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56029.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L1P family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0081.
    KOiK02863.
    OMAiAKITPIA.
    OrthoDBiEOG6FBX2G.

    Family and domain databases

    Gene3Di3.30.190.20. 2 hits.
    3.40.50.790. 1 hit.
    HAMAPiMF_01318_B. Ribosomal_L1_B.
    InterProiIPR005878. Ribosom_L1_bac-type.
    IPR002143. Ribosomal_L1.
    IPR023674. Ribosomal_L1-like.
    IPR028364. Ribosomal_L1/biogenesis.
    IPR016094. Ribosomal_L1_2-a/b-sand.
    IPR016095. Ribosomal_L1_3-a/b-sand.
    IPR023673. Ribosomal_L1_CS.
    [Graphical view]
    PfamiPF00687. Ribosomal_L1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002155. Ribosomal_L1. 1 hit.
    SUPFAMiSSF56808. SSF56808. 1 hit.
    TIGRFAMsiTIGR01169. rplA_bact. 1 hit.
    PROSITEiPS01199. RIBOSOMAL_L1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56029-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKKVFKRLE KLFSKIQNDK AYGVEQGVEV VKSLASAKFD ETVEVALRLG    50
    VDPRHADQMV RGAVVLPHGT GKKVRVAVFA KDIKQDEAKN AGADVVGGDD 100
    LAEEIKNGRI DFDMVIATPD MMAVVGKVGR ILGPKGLMPN PKTGTVTMDI 150
    AKAVSNAKSG QVNFRVDKKG NVHAPIGKAS FPEEKIKENM LELVKTINRL 200
    KPSSAKGKYI RNAALSLTMS PSVSLDAQEL MDIK 234
    Length:234
    Mass (Da):25,266
    Last modified:November 1, 1997 - v1
    Checksum:iBC7CECDB6B0B0EA8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti155 – 1551S → T in strain: Hp921023.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08247.1.
    U86609 Genomic DNA. Translation: AAB47277.1.
    PIRiA64670.
    RefSeqiNP_207992.1. NC_000915.1.
    YP_006935121.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD08247; AAD08247; HP_1201.
    GeneIDi13870401.
    899963.
    KEGGiheo:C694_06215.
    hpy:HP1201.
    PATRICi20593761. VBIHelPyl33062_1258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08247.1 .
    U86609 Genomic DNA. Translation: AAB47277.1 .
    PIRi A64670.
    RefSeqi NP_207992.1. NC_000915.1.
    YP_006935121.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OT0 NMR - A 2-18 [» ]
    1P0G NMR - A 2-20 [» ]
    1P0J NMR - A 2-18 [» ]
    1P0L NMR - A 2-20 [» ]
    1P0O NMR - A 2-20 [» ]
    1P5K NMR - A 2-20 [» ]
    1P5L NMR - A 7-20 [» ]
    ProteinModelPortali P56029.
    SMRi P56029. Positions 7-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 85962.HP1201.

    Proteomic databases

    PRIDEi P56029.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD08247 ; AAD08247 ; HP_1201 .
    GeneIDi 13870401.
    899963.
    KEGGi heo:C694_06215.
    hpy:HP1201.
    PATRICi 20593761. VBIHelPyl33062_1258.

    Phylogenomic databases

    eggNOGi COG0081.
    KOi K02863.
    OMAi AKITPIA.
    OrthoDBi EOG6FBX2G.

    Enzyme and pathway databases

    BioCyci HPY:HP1201-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P56029.

    Family and domain databases

    Gene3Di 3.30.190.20. 2 hits.
    3.40.50.790. 1 hit.
    HAMAPi MF_01318_B. Ribosomal_L1_B.
    InterProi IPR005878. Ribosom_L1_bac-type.
    IPR002143. Ribosomal_L1.
    IPR023674. Ribosomal_L1-like.
    IPR028364. Ribosomal_L1/biogenesis.
    IPR016094. Ribosomal_L1_2-a/b-sand.
    IPR016095. Ribosomal_L1_3-a/b-sand.
    IPR023673. Ribosomal_L1_CS.
    [Graphical view ]
    Pfami PF00687. Ribosomal_L1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002155. Ribosomal_L1. 1 hit.
    SUPFAMi SSF56808. SSF56808. 1 hit.
    TIGRFAMsi TIGR01169. rplA_bact. 1 hit.
    PROSITEi PS01199. RIBOSOMAL_L1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    2. "Isolation of recombinant protective Helicobacter pylori antigens."
      Hocking D., Webb E., Radcliff F., Rothel L., Taylor S., Pinczower G., Kapouleas C., Braley H., Lee A., Doidge C.
      Infect. Immun. 67:4713-4719(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-234.
      Strain: Hp921023.
    3. Cited for: SYNTHESIS, ANTIBACTERIAL ACTIVITY OF 2-20 AND 22-38.
    4. "Structure of antimicrobial peptide, HP (2-20) and its analogues derived from Helicobacter pylori, as determined by 1H NMR spectroscopy."
      Lee K.H., Lee D.G., Park Y., Hahm K.-S., Kim Y.
      Submitted (SEP-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 2-20.
    5. "Interactions between the plasma membrane and the antimicrobial peptide HP (2-20) and its analogues derived from Helicobacter pylori."
      Lee K.H., Lee D.G., Park Y., Kang D.-I., Shin S.Y., Hahm K.-S., Kim Y.
      Biochem. J. 394:105-114(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 2-20, ANTIFUNGAL ACTIVITY, LYSIS MECHANISM, MUTAGENESIS OF 17-GLN--ASP-19.

    Entry informationi

    Entry nameiRL1_HELPY
    AccessioniPrimary (citable) accession number: P56029
    Secondary accession number(s): P94849
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The antimicrobial peptide HP (2-20) forms a short alpha-helix. Increasing the amphiphilicity of the helix increases its antimicrobial activities.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Ribosomal proteins
      Ribosomal proteins families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3