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Protein

50S ribosomal protein L1

Gene

rplA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.UniRule annotation
Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.UniRule annotation
Peptides originating from the N-terminal end of L1 have antibacterial activity against bacteria such as E.coli and B.megaterium and modest antifungal activities. Has no effect on H.pylori itself. Peptides are not hemolytic against mammalian cells. These peptides may be released in the stomach during altruistic lysis to kill other fast growing bacteria.

Miscellaneous

The antimicrobial peptide HP (2-20) forms a short alpha-helix. Increasing the amphiphilicity of the helix increases its antimicrobial activities.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Repressor, Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding
Biological processTranslation regulation

Enzyme and pathway databases

BioCyciHPY:HP1201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L1UniRule annotation
Gene namesi
Name:rplAUniRule annotation
Ordered Locus Names:HP_1201
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17 – 19QND → WNW: Increases antibacterial and antifungal activities 2-4 fold without an increase in hemolytic activies. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001256671 – 23450S ribosomal protein L1Add BLAST234

Proteomic databases

PaxDbiP56029.

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit.

Protein-protein interaction databases

STRINGi85962.HP1201.

Structurei

Secondary structure

1234
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 18Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OT0NMR-A2-18[»]
1P0GNMR-A2-20[»]
1P0JNMR-A2-18[»]
1P0LNMR-A2-20[»]
1P0ONMR-A2-20[»]
1P5KNMR-A2-20[»]
1P5LNMR-A7-20[»]
ProteinModelPortaliP56029.
SMRiP56029.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56029.

Family & Domainsi

Sequence similaritiesi

Belongs to the universal ribosomal protein uL1 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C64. Bacteria.
COG0081. LUCA.
KOiK02863.
OMAiGWTDVDV.

Family and domain databases

CDDicd00403. Ribosomal_L1. 1 hit.
Gene3Di3.40.50.790. 1 hit.
HAMAPiMF_01318_B. Ribosomal_L1_B. 1 hit.
InterProiView protein in InterPro
IPR005878. Ribosom_L1_bac-type.
IPR002143. Ribosomal_L1.
IPR023674. Ribosomal_L1-like.
IPR028364. Ribosomal_L1/biogenesis.
IPR016095. Ribosomal_L1_3-a/b-sand.
IPR023673. Ribosomal_L1_CS.
PfamiView protein in Pfam
PF00687. Ribosomal_L1. 1 hit.
PIRSFiPIRSF002155. Ribosomal_L1. 1 hit.
SUPFAMiSSF56808. SSF56808. 1 hit.
TIGRFAMsiTIGR01169. rplA_bact. 1 hit.
PROSITEiView protein in PROSITE
PS01199. RIBOSOMAL_L1. 1 hit.

Sequencei

Sequence statusi: Complete.

P56029-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVFKRLE KLFSKIQNDK AYGVEQGVEV VKSLASAKFD ETVEVALRLG
60 70 80 90 100
VDPRHADQMV RGAVVLPHGT GKKVRVAVFA KDIKQDEAKN AGADVVGGDD
110 120 130 140 150
LAEEIKNGRI DFDMVIATPD MMAVVGKVGR ILGPKGLMPN PKTGTVTMDI
160 170 180 190 200
AKAVSNAKSG QVNFRVDKKG NVHAPIGKAS FPEEKIKENM LELVKTINRL
210 220 230
KPSSAKGKYI RNAALSLTMS PSVSLDAQEL MDIK
Length:234
Mass (Da):25,266
Last modified:November 1, 1997 - v1
Checksum:iBC7CECDB6B0B0EA8
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti155S → T in strain: Hp921023. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08247.1.
U86609 Genomic DNA. Translation: AAB47277.1.
PIRiA64670.
RefSeqiNP_207992.1. NC_000915.1.
WP_001085839.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08247; AAD08247; HP_1201.
GeneIDi899963.
KEGGiheo:C694_06215.
hpy:HP1201.
PATRICifig|85962.47.peg.1291.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRL1_HELPY
AccessioniPrimary (citable) accession number: P56029
Secondary accession number(s): P94849
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 7, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families