Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

BioCyciHPY:HP1293-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:HP_1293
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001753161 – 344DNA-directed RNA polymerase subunit alphaAdd BLAST344

Proteomic databases

PaxDbiP56001

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-3396N
IntActiP56001, 2 interactors
MINTiP56001
STRINGi85962.HP1293

Structurei

3D structure databases

ProteinModelPortaliP56001
SMRiP56001
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 238Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST238
Regioni254 – 344Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST91

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTF Bacteria
COG0202 LUCA
KOiK03040
OMAiLMKFRNF

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit

Sequencei

Sequence statusi: Complete.

P56001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIKTAPLI PSEIKVLEKE GNRVKISLAP FEFGYAVTLA HPIRRLLLLS
60 70 80 90 100
SVGYAPVGLK IEGVHHEFDS LRGVTEDVSL FIMNLKNIRF IAKALVGQDS
110 120 130 140 150
SLENQSVVVD YSFKGPMELR ARDLNSEQIE IVNPEMPLAT INEDAQLNFS
160 170 180 190 200
LIIYKGMGYV PSENTRELMP EGYMPLDGSF TPIKKVVYEI ENVLVEGDPN
210 220 230 240 250
YEKIIFDIET DGQIDPYKAF LSAVKVMSKQ LGVFGERPIA NTEYSGDYAQ
260 270 280 290 300
RDDAKDLSAK IESMNLSARC FNCLDKIGIK YVGELVLMSE EELKGVKNMG
310 320 330 340
KKSYDEIAEK LNDLGYPVGT ELSPEQRESL KKRLEKLEDK GGND
Length:344
Mass (Da):38,499
Last modified:November 1, 1997 - v1
Checksum:i0F812B0020DF7414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD08336.1
PIRiE64681
RefSeqiNP_208085.1, NC_000915.1
WP_000864547.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD08336; AAD08336; HP_1293
GeneIDi898788
KEGGiheo:C694_06680
hpy:HP1293
PATRICifig|85962.47.peg.1387

Similar proteinsi

Entry informationi

Entry nameiRPOA_HELPY
AccessioniPrimary (citable) accession number: P56001
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health