P56001 (RPOA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit alpha
Short name=RNAP subunit alpha
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha

Gene names

Name:	rpoA
Ordered Locus Names:	HP_1293

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_00059
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059
Subunit structure	Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Domain	The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. HAMAP-Rule MF_00059
Sequence similarities	Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	DNA-directed RNA polymerase subunit alpha HAMAP-Rule MF_00059		PRO_0000175316
Regions
	Region	1 – 238	238	Alpha N-terminal domain (alpha-NTD) By similarity
	Region	254 – 344	91	Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56001 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0F812B0020DF7414	FASTA	344	38,499
	10 20 30 40 50 60 MKVIKTAPLI PSEIKVLEKE GNRVKISLAP FEFGYAVTLA HPIRRLLLLS SVGYAPVGLK 70 80 90 100 110 120 IEGVHHEFDS LRGVTEDVSL FIMNLKNIRF IAKALVGQDS SLENQSVVVD YSFKGPMELR 130 140 150 160 170 180 ARDLNSEQIE IVNPEMPLAT INEDAQLNFS LIIYKGMGYV PSENTRELMP EGYMPLDGSF 190 200 210 220 230 240 TPIKKVVYEI ENVLVEGDPN YEKIIFDIET DGQIDPYKAF LSAVKVMSKQ LGVFGERPIA 250 260 270 280 290 300 NTEYSGDYAQ RDDAKDLSAK IESMNLSARC FNCLDKIGIK YVGELVLMSE EELKGVKNMG 310 320 330 340 KKSYDEIAEK LNDLGYPVGT ELSPEQRESL KKRLEKLEDK GGND

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08336.1.
PIR	E64681.
RefSeq	NP_208085.1. NC_000915.1. YP_006935214.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56001.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3396N.
MINT	MINT-172290.
STRING	85962.HP1293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08336; AAD08336; HP_1293.
GeneID	13870501. 898788.
KEGG	heo:C694_06680. hpy:HP1293.
PATRIC	20593967. VBIHelPyl33062_1354.
Phylogenomic databases
eggNOG	COG0202.
KO	K03040.
OMA	GKKSYDE.
ProtClustDB	PRK05182.
Family and domain databases
Gene3D	2.170.120.12. 1 hit.
HAMAP	MF_00059. RNApol_bact_RpoA.
InterPro	IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011773. DNA-dir_RpoA. IPR011260. RNAP_asu_C. [Graphical view]
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
ProDom	PD001179. RNAP_asu_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
SUPFAM	SSF47789. RNAP_alpha_C. 1 hit. SSF56553. RNAP_insert. 1 hit. SSF55257. RNAP_RBP11-like. 1 hit.
TIGRFAMs	TIGR02027. rpoA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA_HELPY

Accession

Primary (citable) accession number: P56001

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program