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P56001 (RPOA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit alpha

Short name=RNAP subunit alpha
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha
Gene names
Name:rpoA
Ordered Locus Names:HP_1293
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_00059

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059

Subunit structure

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Domain

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. HAMAP-Rule MF_00059

Sequence similarities

Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344DNA-directed RNA polymerase subunit alpha HAMAP-Rule MF_00059
PRO_0000175316

Regions

Region1 – 238238Alpha N-terminal domain (alpha-NTD) By similarity
Region254 – 34491Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

Sequence LengthMass (Da)Tools
P56001 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 0F812B0020DF7414

FASTA34438,499
        10         20         30         40         50         60 
MKVIKTAPLI PSEIKVLEKE GNRVKISLAP FEFGYAVTLA HPIRRLLLLS SVGYAPVGLK 

        70         80         90        100        110        120 
IEGVHHEFDS LRGVTEDVSL FIMNLKNIRF IAKALVGQDS SLENQSVVVD YSFKGPMELR 

       130        140        150        160        170        180 
ARDLNSEQIE IVNPEMPLAT INEDAQLNFS LIIYKGMGYV PSENTRELMP EGYMPLDGSF 

       190        200        210        220        230        240 
TPIKKVVYEI ENVLVEGDPN YEKIIFDIET DGQIDPYKAF LSAVKVMSKQ LGVFGERPIA 

       250        260        270        280        290        300 
NTEYSGDYAQ RDDAKDLSAK IESMNLSARC FNCLDKIGIK YVGELVLMSE EELKGVKNMG 

       310        320        330        340 
KKSYDEIAEK LNDLGYPVGT ELSPEQRESL KKRLEKLEDK GGND 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08336.1.
PIRE64681.
RefSeqNP_208085.1. NC_000915.1.
YP_006935214.1. NC_018939.1.

3D structure databases

ProteinModelPortalP56001.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3396N.
IntActP56001. 2 interactions.
MINTMINT-172290.
STRING85962.HP1293.

Proteomic databases

PRIDEP56001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08336; AAD08336; HP_1293.
GeneID898788.
KEGGheo:C694_06680.
hpy:HP1293.
PATRIC20593967. VBIHelPyl33062_1354.

Phylogenomic databases

eggNOGCOG0202.
KOK03040.
OMADDGLEFN.
OrthoDBEOG68WR84.

Enzyme and pathway databases

BioCycHPY:HP1293-MONOMER.

Family and domain databases

Gene3D2.170.120.12. 1 hit.
HAMAPMF_00059. RNApol_bact_RpoA.
InterProIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsTIGR02027. rpoA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRPOA_HELPY
AccessionPrimary (citable) accession number: P56001
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names