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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciHPY:HP1293-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:HP_1293
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344DNA-directed RNA polymerase subunit alphaPRO_0000175316Add
BLAST

Proteomic databases

PRIDEiP56001.

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3396N.
IntActiP56001. 2 interactions.
MINTiMINT-172290.
STRINGi85962.HP1293.

Structurei

3D structure databases

ProteinModelPortaliP56001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 238238Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd
BLAST
Regioni254 – 34491Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd
BLAST

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0202.
KOiK03040.
OMAiRCFNCLD.
OrthoDBiEOG68WR84.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.

Sequencei

Sequence statusi: Complete.

P56001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIKTAPLI PSEIKVLEKE GNRVKISLAP FEFGYAVTLA HPIRRLLLLS
60 70 80 90 100
SVGYAPVGLK IEGVHHEFDS LRGVTEDVSL FIMNLKNIRF IAKALVGQDS
110 120 130 140 150
SLENQSVVVD YSFKGPMELR ARDLNSEQIE IVNPEMPLAT INEDAQLNFS
160 170 180 190 200
LIIYKGMGYV PSENTRELMP EGYMPLDGSF TPIKKVVYEI ENVLVEGDPN
210 220 230 240 250
YEKIIFDIET DGQIDPYKAF LSAVKVMSKQ LGVFGERPIA NTEYSGDYAQ
260 270 280 290 300
RDDAKDLSAK IESMNLSARC FNCLDKIGIK YVGELVLMSE EELKGVKNMG
310 320 330 340
KKSYDEIAEK LNDLGYPVGT ELSPEQRESL KKRLEKLEDK GGND
Length:344
Mass (Da):38,499
Last modified:November 1, 1997 - v1
Checksum:i0F812B0020DF7414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08336.1.
PIRiE64681.
RefSeqiNP_208085.1. NC_000915.1.
YP_006935214.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08336; AAD08336; HP_1293.
GeneIDi13870501.
898788.
KEGGiheo:C694_06680.
hpy:HP1293.
PATRICi20593967. VBIHelPyl33062_1354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08336.1.
PIRiE64681.
RefSeqiNP_208085.1. NC_000915.1.
YP_006935214.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3396N.
IntActiP56001. 2 interactions.
MINTiMINT-172290.
STRINGi85962.HP1293.

Proteomic databases

PRIDEiP56001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08336; AAD08336; HP_1293.
GeneIDi13870501.
898788.
KEGGiheo:C694_06680.
hpy:HP1293.
PATRICi20593967. VBIHelPyl33062_1354.

Phylogenomic databases

eggNOGiCOG0202.
KOiK03040.
OMAiRCFNCLD.
OrthoDBiEOG68WR84.

Enzyme and pathway databases

BioCyciHPY:HP1293-MONOMER.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiRPOA_HELPY
AccessioniPrimary (citable) accession number: P56001
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.