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P56000 (SYV_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Valine--tRNA ligase
EC=6.1.1.9
Alternative name(s):
Valyl-tRNA synthetase
Short name=ValRS
Gene names
Name:valS
Ordered Locus Names:HP_1153
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874Valine--tRNA ligase HAMAP-Rule MF_02004
PRO_0000106227

Regions

Coiled coil813 – 87361 Potential
Motif51 – 6111"HIGH" region HAMAP-Rule MF_02004
Motif533 – 5375"KMSKS" region HAMAP-Rule MF_02004

Sites

Binding site5361ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P56000 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: BB5668E2ACD5AD28

FASTA874101,360
        10         20         30         40         50         60 
MIMKQEPTTY QPEEIEKKIY EICSHRGYFE IDGNEAIQEK NKRFCLMMPP PNVTGVLHIG 

        70         80         90        100        110        120 
HALTLSLQDI LARYKRMDGY KTLYQPGLDH AGIATQNVVE KQLLSQGIKK EDLGREEFIK 

       130        140        150        160        170        180 
KVWEWKEKSG GAILEQMKRL GVSAAFSRTR FTMDKGLQRA VKLAFLKWYE KGLIIQDNYM 

       190        200        210        220        230        240 
VNWCTKDGAL SDIEVEYEER KGALYYIRYY LENQKDYLVV ATTRPETLFG DSALMVNPND 

       250        260        270        280        290        300 
ERYKHLVGQK AILPLIHRTI PIIADEHVEM EFGTGCVKVT PGHDFNDYEV GKRHHLETIK 

       310        320        330        340        350        360 
IFDEKGILNA HCGEFENLER LEARDKVVER LKENALLEKI EEHTHQVGHC YRCHNVVEPY 

       370        380        390        400        410        420 
VSKQWFVKPE IAQSSIEKIQ QGLARFYPSN WINNYNAWMR ELRPWCISRQ LFWGHQIPVF 

       430        440        450        460        470        480 
TCENNHQFVS LDTPLSCPTC KSETLEQDKD VLDTWFSSGL WAFSTLGWGQ EKSGLFNESD 

       490        500        510        520        530        540 
LKDFYPNTTL ITGFDILFFW VARMLFCSES LLGELPFKDI YLHALVRDEK GEKMSKSKGN 

       550        560        570        580        590        600 
VIDPLEMIEK YGADSLRFTL ANLCATGRDI KLSTTHLENN KNFANKLFNA ASYLKLKQES 

       610        620        630        640        650        660 
FKDKERLNEY QTPLGRYAKS RLNSATKEAR NALDNYRFND ATTLLYRFLW GEFCDWFIEF 

       670        680        690        700        710        720 
SKVENEAIDE LGSVLKEALK LLHPFMPFIS ESLYHKLSNT ELENTESIMV MPYPKDLAQD 

       730        740        750        760        770        780 
EKLEHEFEVI KDCIVSLRRL KIMLETPPIV LKEASVGLRE AIENTERLQT YAQKLARLEK 

       790        800        810        820        830        840 
VSVISSKPLK SVSDVGEFCQ TYANLENLDL SPLVARLKKQ LEKLEKEKLK LNLHNENFVK 

       850        860        870 
NAPKSVLEKA KESLKTLLEK ESKIKQELDL LEQP

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000511 Genomic DNA. Translation: AAD08195.1.
PIRA64664.
RefSeqNP_207944.1. NC_000915.1.

3D structure databases

ProteinModelPortalP56000.
ModBaseSearch...

Protein-protein interaction databases

STRING85962.HP1153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08195; AAD08195; HP_1153.
GeneID899913.
KEGGhpy:HP1153.
PATRIC20593657. VBIHelPyl33062_1207.

Phylogenomic databases

eggNOGCOG0525.
KOK01873.
OMADPLEMIE.
ProtClustDBPRK05729.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYV_HELPY
AccessionPrimary (citable) accession number: P56000
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

SIMILARITY comments

Index of protein domains and families

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