P55999 (RF2_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 2
Short name=RF-2

Gene names

Name:	prfB
Ordered Locus Names:	HP_0171

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA By similarity. HAMAP MF_00094
Subcellular location	Cytoplasm HAMAP MF_00094.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF2 By similarity. HAMAP MF_00094
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation release factor activity, codon specific Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Peptide chain release factor 2 HAMAP MF_00094		PRO_0000166821
Amino acid modifications
	Modified residue	251	1	N5-methylglutamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55999 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 7DE21BC84D69DA9E	FASTA	363	41,317
	10 20 30 40 50 60 MDNYTYSELL KSLQNKCDNI ALIIKPEKIK QELERIEKEQ EDPNFWQDVL KARDTNKEKV 70 80 90 100 110 120 RLNRLLETYQ KTKNSLDESV ELFELAQNDN DEVTLSLLYE EAPILENSVQ KVEIEIMLSG 130 140 150 160 170 180 ENDASNAIIT IQPGAGGTES QDWASILYRM YLRWAERRGF KSEILDYQDG EEAGIKGVAF 190 200 210 220 230 240 IIKGENAYGY LKNENGVHRL VRISPFDANA KRHTSFASVQ ISPELDDDID IEIDEKDVRY 250 260 270 280 290 300 DYYRSSGAGG QHVNKTESAV RITHFPTGIV VQCQNDRSQH KNKASALKML KSKLYELELE 310 320 330 340 350 360 KQQSSAKNEE KSEIGWGHQI RSYVLAPYQQ VKDARSNTAY SNVEAILDGD IDAILEGVLI AKA

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07236.1.
PIR	C64541.
RefSeq	NP_206970.1. NC_000915.1.
3D structure databases
ProteinModelPortal	P55999.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	899148.
GenomeReviews	Gene locus HP_0171 in contig AE000511_GR.
KEGG	hpy:HP0171.
NMPDR	fig|85962.1.peg.168.
PATRIC	20591563. VBIHelPyl33062_0181.
TIGR	HP_0171.
Phylogenomic databases
HOGENOM	HBG629764.
OMA	FELAQND.
ProtClustDB	PRK00578.
Family and domain databases
HAMAP	MF_00094. Rel_fac_2. [Tree]
InterPro	IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR020853. Peptide_chain_release_fac2_bac. IPR004374. PrfB. [Graphical view]
KO	K02836.
PANTHER	PTHR11075:SF6. PrfB. 1 hit.
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00020. PrfB. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF2_HELPY

Accession

Primary (citable) accession number: P55999

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program