P55995 (LON_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	HP_1379

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	835 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 835	835	Lon protease		PRO_0000076137
Regions
	Domain	9 – 197	189	Lon
	Nucleotide binding	367 – 374	8	ATP By similarity
	Compositional bias	712 – 716	5	Poly-Lys
Sites
	Active site	741	1	By similarity
	Active site	784	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55995 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4BDA52814191C727	FASTA	835	94,419
	10 20 30 40 50 60 MTEDFPKILP LLVEEDTFLY PFMIAPIFLQ NNASIKAVAY AKNNKSLVFI ACQKDKLNDN 70 80 90 100 110 120 EAPYYDVGVI GSVMREANMP NGRVKLLFNG IAKGRILEPA KENEQGFLEA QISPIEYLEY 130 140 150 160 170 180 DKENIQAIVE VLKEKVITLA NVSSLFPPDL IKALEDNDDP NRIADLIAAA LHLKKDQAYS 190 200 210 220 230 240 LFANNNTEQR LLDLIDIVIE ETKTQKLQKE IKSKVHQKME QTNKEYFLKE QLKQIQKELG 250 260 270 280 290 300 TDKQRDEDLN QYYQKLESIK PFLKEEAFKE IKKQIDRLSR THADSSDSAT LQNYIETMLD 310 320 330 340 350 360 VPFGQYGKKA LDIKHVREQL DKDHYSLKRP KERIVEYFAT MQLLEMRRKK KPEKKDKTKG 370 380 390 400 410 420 TILCFYGPPG VGKTSLANSI AKAIERPLVR IALGGLEDVN ELRGHRRTYI GSMPGRIVQG 430 440 450 460 470 480 LIEAKKMNPV MVLDEIDKVD RSVRGDPASA LLEILDPEQN TAFRDHYANF SIDLSQVIFI 490 500 510 520 530 540 ATANNIDRIP APLRDRMEFI SVSSYTPNEK EEIAKNYLIP QELEKHALKP SEVEISHECL 550 560 570 580 590 600 KLIIEKYTRE AGVRDLRRQI ATIMRKVALK YLEDNPHQKG RTKKGKNEKS EDQKSEDQKS 610 620 630 640 650 660 ENQKSENKDF CVSITPNNLK EYLERMVFEI DPIDEENKIG IVNGLAWTPV GGDVLKIEVL 670 680 690 700 710 720 KIRGKGELKL TGSLGDVMKE SAIIAFSVVK VLLDNETLKV PKIPSETDAE GKKKKKVLKV 730 740 750 760 770 780 YNAYDLHLHV PEGATPKDGP SAGIAMASVM ASILCDRATR SEVAMTGELT LSGEVLPIGG 790 800 810 820 830 LKEKLIAAFK AGIKTALIPV KNYERDLDEI PAEVRENLNI VAVKNIAEVL EKTLL

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08421.1.
PIR	C64692.
RefSeq	NP_208170.1. NC_000915.1.
3D structure databases
ProteinModelPortal	P55995.
SMR	P55995. Positions 638-834.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3184N.
MINT	MINT-186118.
Protein family/group databases
MEROPS	S16.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	900303.
GenomeReviews	Gene locus HP_1379 in contig AE000511_GR.
KEGG	hpy:HP1379.
NMPDR	fig|85962.1.peg.1366.
PATRIC	20594147. VBIHelPyl33062_1444.
TIGR	HP_1379.
Phylogenomic databases
HOGENOM	HBG566281.
OMA	GTILCFY.
ProtClustDB	CLSK872549.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_HELPY

Accession

Primary (citable) accession number: P55995

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program