Reviewed, UniProtKB/Swiss-Prot P55994 (DNAK_HELPY)

Names and origin

Protein names

Recommended name:
    Chaperone protein dnaK
Alternative name(s):
    Heat shock protein 70
    Heat shock 70 kDa protein
    HSP70

Gene names

Name:	dnaK
Ordered Locus Names:	HP_0109

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts as a chaperone By similarity.
Induction	By stress conditions e.g. heat shock By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 620	619	Chaperone protein dnaK HAMAP MF_00332		PRO_0000078470
Amino acid modifications
	Modified residue	197	1	Phosphothreonine; by autocatalysis By similarity
Experimental info
	Sequence conflict	26 – 29	4	IIAN → DLLRI in AAB53800. Ref.2
	Sequence conflict	108	1	V → I in AAB53800. Ref.2
	Sequence conflict	151	1	K → Q in AAB53800. Ref.2
	Sequence conflict	155	1	E → D in AAB53800. Ref.2
	Sequence conflict	177	1	A → L in AAB53800. Ref.2
	Sequence conflict	307 – 308	2	MK → VE in AAB53800. Ref.2
	Sequence conflict	316	1	S → G in AAB53800. Ref.2
	Sequence conflict	354	1	D → E in AAB53800. Ref.2
	Sequence conflict	417	1	S → SS in AAB53800. Ref.2
	Sequence conflict	430 – 435	6	VSIMVL → GVPYGF in AAB53800. Ref.2
	Sequence conflict	502 – 507	6	SDSEIE → TIAK in AAB53800. Ref.2
	Sequence conflict	522	1	K → R in AAB53800. Ref.2
	Sequence conflict	570 – 580	11	VKNDNATKAEL → IKTITLLKRI in AAB53800. Ref.2
	Sequence conflict	586 – 600	15	LLAQA…EAMAN → ALKGSKIRRNGE Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55994-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8C5E42ECC0558481	FASTA	620	67,052
	10 20 30 40 50 60 MGKVIGIDLG TTNSAMAVYE GNEAKIIANK EGKNTTPSIV AFTDKGEILV GESAKRQAVT 70 80 90 100 110 120 NPEKTIYSIK RIMGLMFNED KAKEAEKRLP YKIVDRNGAC AIEISGKVYT PQEISAKILM 130 140 150 160 170 180 KLKEDAESYL GESVTEAVIT VPAYFNDSQR KATKEAGTIA GLNVLRIINE PTSAALAYGL 190 200 210 220 230 240 DKKESEKIMV YDLGGGTFDV TVLETGDNVV EVLATGGDAF LGGDDFDNRV IDFLASEFKS 250 260 270 280 290 300 ETGIEIKNDV MALQRLKEAA ENAKKELSSA METEINLPFI TADATGPKHL VKKLTRAKFE 310 320 330 340 350 360 SLTEDLMKET ISKIESVIKD AGLTKNEISE VVMVGGSTRI PKVQERVKAF INKDLNKSVN 370 380 390 400 410 420 PDEVVAVGAS IQGGVLKGDV KDVLLLDVTP LSLGIETLGG VMTKVIDRGT TIPAKKSQVF 430 440 450 460 470 480 STAEDNQPAV SIMVLQGERE LARDNKSLGK FDLQGIAPAP RGVPQIEVTF DIDANGILTV 490 500 510 520 530 540 SAQDKNTGKS QEIKISGSSG LSDSEIEKMV KDAELHKEED AKKKEVIEAR NHADSLAHQT 550 560 570 580 590 600 QKSLDEHKTN LNENDANEIQ NAINALKDCV KNDNATKAEL EDKTKLLAQA AQKLGEAMAN 610 620 KNNAEQPKKK DDDVIDAEVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Characterization of an acidic-pH-inducible stress protein (hsp70), a putative sulfatide binding adhesin, from Helicobacter pylori." Huesca M., Goodwin A., Bhagwansingh A., Hoffman P., Lingwood C.A. Infect. Immun. 66:4061-4067(1998) [PubMed: 9712748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07178.1. U96271 Genomic DNA. Translation: AAB53800.1.
PIR	E64533.
RefSeq	NP_206909.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3190N.
Genome annotation databases
GeneID	900161.
GenomeReviews	Gene locus HP_0109 in contig AE000511_GR.
KEGG	hpy:HP0109.
NMPDR	fig|85962.1.peg.107.
TIGR	HP_0109.
Phylogenomic databases
HOGENOM	HBG334976.
OMA	ERIVGRQ.
Family and domain databases
HAMAP	MF_00332. [Tree]
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAK_HELPY

Accession

Primary (citable) accession number: P55994
Secondary accession number(s): O05733

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)