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P55991 (TOP1_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:HP_0116
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

flgBO260813EBI-7493738,EBI-7500288

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 736736DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145150

Regions

Domain2 – 113112Toprim
Zinc finger572 – 59827C4-type 1 HAMAP-Rule MF_00952
Zinc finger616 – 64227C4-type 2 HAMAP-Rule MF_00952
Zinc finger663 – 68927C4-type 3 HAMAP-Rule MF_00952
Zinc finger702 – 72524C4-type 4 HAMAP-Rule MF_00952
Region163 – 1686Interaction with DNA By similarity

Sites

Active site2971O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium 1; catalytic By similarity
Metal binding821Magnesium 1; catalytic By similarity
Metal binding821Magnesium 2 By similarity
Metal binding841Magnesium 2 By similarity
Site321Interaction with DNA By similarity
Site1391Interaction with DNA By similarity
Site1401Interaction with DNA By similarity
Site1431Interaction with DNA By similarity
Site2991Interaction with DNA By similarity
Site4841Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P55991 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 357CE39735F48475

FASTA73683,196
        10         20         30         40         50         60 
MKHLIIVESP AKAKTIKNFL DKNYEVIASK GHVRDLSKFA LGIKIDETGF TPNYVVDKDH 

        70         80         90        100        110        120 
KELVKQIIEL SKKASITYIA TDEDREGEAI GYHVACLIGG KLESYPRIVF HEITQNAILN 

       130        140        150        160        170        180 
ALKTPRKIDM SKVNAQQARR FLDRIVGFKL SSLIASKITK GLSAGRVQSA ALKLVIDKER 

       190        200        210        220        230        240 
EIKAFKPLTY FTLDAYFESH LEAQLISYKG NKLKAQELID EKKAQEIKNE LEKESYAISS 

       250        260        270        280        290        300 
IVKKSKKSPT PPPFMTSTLQ QSASSLLGFS PTKTMSIAQK LYEGVATPQG VMGVITYMRT 

       310        320        330        340        350        360 
DSLNIAKEAL EEARNKILKD YGKDYLPPKA KVYSSKNKNA QEAHEAIRPT SIILEPNALK 

       370        380        390        400        410        420 
DYLKPEELRL YTLIYKRFLA SQMQDALFES QSVVVACEKG EFKASGRKLL FDGYYKILGN 

       430        440        450        460        470        480 
DDKDKLLPNL KENDPIKLEK LESNAHVTEP PARYSEASLI KVLESLGIGR PSTYAPTISL 

       490        500        510        520        530        540 
LQNRDYIKVE KKQISALESA FKVIEILEKH FEEIVDSKFS ASLEEELDNI AQNKADYQQV 

       550        560        570        580        590        600 
LKDFYYPFMD KIEAGKKNII SQKVHEKTGQ SCPKCGGELV KKNSRYGEFI ACNNYPKCKY 

       610        620        630        640        650        660 
VKQTESANDE ADQELCEKCG GEMVQKFSRN GAFLACNNYP ECKNTKSLKN TPNAKETIEG 

       670        680        690        700        710        720 
VKCPECGGDI ALKRSKKGSF YGCNNYPKCN FLSNHKPINK RCEKCHYLMS ERIYRKKKAH 

       730 
ECIKCKERVF LEEDNG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07184.1.
PIRD64534.
RefSeqNP_206916.1. NC_000915.1.
YP_006934039.1. NC_018939.1.

3D structure databases

ProteinModelPortalP55991.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3057N.
IntActP55991. 16 interactions.
MINTMINT-182749.
STRING85962.HP0116.

Proteomic databases

PRIDEP55991.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07184; AAD07184; HP_0116.
GeneID898836.
KEGGheo:C694_00575.
hpy:HP0116.
PATRIC20591447. VBIHelPyl33062_0123.

Phylogenomic databases

eggNOGCOG0551.
KOK03168.
OMAQNDPIEP.
OrthoDBEOG6S7XQ9.

Enzyme and pathway databases

BioCycHPY:HP0116-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_HELPY
AccessionPrimary (citable) accession number: P55991
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names