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P55963

- CA4A_CONPU

UniProt

P55963 - CA4A_CONPU

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Protein

Alpha-conotoxin PIVA

Gene
N/A
Organism
Conus purpurascens (Purple cone)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin has higher affinity for the adult subtype (alpha-1/beta-1/gamma/delta subunits) (IC50=2.3 nM) of the receptor than for the fetal subtype (alpha-1/beta-1/epsilon/delta subunits) (IC50=22 nM).1 Publication

GO - Molecular functioni

  1. acetylcholine receptor inhibitor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin PIVA
OrganismiConus purpurascens (Purple cone)
Taxonomic identifieri41690 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri1449. PIVA.
1612. PIVA [Hyp7P,Hyp13P].

Subcellular locationi

GO - Cellular componenti

  1. other organism postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 2525Alpha-conotoxin PIVAPRO_0000044466Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 162 Publications
Disulfide bondi3 ↔ 112 Publications
Modified residuei7 – 714-hydroxyproline; partial1 Publication
Modified residuei13 – 1314-hydroxyproline; partial1 Publication
Disulfide bondi14 ↔ 232 Publications
Modified residuei20 – 2014-hydroxyproline1 Publication
Modified residuei25 – 251Glutamine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

1
25
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni13 – 153Combined sources
Helixi21 – 233Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P1PNMR-A1-25[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55963.

Family & Domainsi

Domaini

The cysteine framework is IV (CC-C-C-C-C).

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Family and domain databases

InterProiIPR012498. Toxin_14.
[Graphical view]
PfamiPF07829. Toxin_14. 1 hit.
[Graphical view]
ProDomiPD045217. Toxin_14. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P55963-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20 
GCCGSYPNAA CHPCSCKDRP SYCGQ
Length:25
Mass (Da):2,608
Last modified:November 1, 1997 - v1
Checksum:i9E2147898D697640
GO

Sequence databases

PIRiA58647.

Cross-referencesi

Sequence databases

PIRi A58647.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1P1P NMR - A 1-25 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ConoServeri 1449. PIVA.
1612. PIVA [Hyp7P,Hyp13P].

Miscellaneous databases

EvolutionaryTracei P55963.

Family and domain databases

InterProi IPR012498. Toxin_14.
[Graphical view ]
Pfami PF07829. Toxin_14. 1 hit.
[Graphical view ]
ProDomi PD045217. Toxin_14. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "A new family of Conus peptides targeted to the nicotinic acetylcholine receptor."
    Hopkins C., Grilley M., Miller C., Shon K.-J., Cruz L.J., Gray W.R., Dykert J., Rivier J.E., Yoshikami D., Olivera B.M.
    J. Biol. Chem. 270:22361-22367(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, HYDROXYLATION AT PRO-7; PRO-13 AND PRO-20, AMIDATION AT GLN-25.
    Tissue: Venom.
  2. "Definition and characterization of the short alphaA-conotoxins: a single residue determines dissociation kinetics from the fetal muscle nicotinic acetylcholine receptor."
    Teichert R.W., Lopez-Vera E., Gulyas J., Watkins M., Rivier J., Olivera B.M.
    Biochemistry 45:1304-1312(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SYNTHESIS, FUNCTION.
  3. "NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA."
    Han K.-H., Hwang K.-J., Kim S.-M., Kim S.-K., Gray W.R., Olivera B.M., Rivier J.E., Shon K.-J.
    Biochemistry 36:1669-1677(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Entry informationi

Entry nameiCA4A_CONPU
AccessioniPrimary (citable) accession number: P55963
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3