P55956 (ASP3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartic protease 3
EC=3.4.23.-

Gene names

Name:	asp-3
ORF Names:	H22K11.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell death Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 55	38	Removed in mature form		PRO_0000025937
	Chain	56 – 398	343	Aspartic protease 3		PRO_0000025938
Sites
	Active site	87	1	By similarity
	Active site	279	1	By similarity
Amino acid modifications
	Glycosylation	321	1	N-linked (GlcNAc...) Ref.3
	Disulfide bond	100 ↔ 107		By similarity
	Disulfide bond	313 ↔ 351		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55956 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: 4A16251D2CB14121	FASTA	398	43,413
	10 20 30 40 50 60 MSGRVFLLLA LVALASAIQR IKLEKRTYTR EQYKFGSIQE HLKAKYVPGY IPNKDAFNEG 70 80 90 100 110 120 LSDYSNAQYY GPVTIGTPPQ NFQVLFDTGS SNLWVPCANC PFGDIACRMH NRFDCKKSSS 130 140 150 160 170 180 CTATGASFEI QYGTGSMKGT VDNDVVCFGH DTTYCTDKNQ GLACATSEPG ITFVAAKFDG 190 200 210 220 230 240 IFGMGWDTIS VNKISQPMDQ IFANSAICKN QLFAFWLSRD ANDITNGGEI TLCETDPNHY 250 260 270 280 290 300 VGNIAWEPLV SEDYWRIKLA SVVIDGTTYT SGPIDSIVDT GTSLLTGPTD VIKKIQHKIG 310 320 330 340 350 360 GIPLFNGEYE VECSKIPSLP NITFNLGGQN FDLQGKDYIL QMSNGNGGST CLSGFMGMDI 370 380 390 PAPAGPLWIL GDVFIGRFYS VFDHGNKRVG FATSRTGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Two-dimensional gel electrophoresis of Caenorhabditis elegans homogenates and identification of protein spots by microsequencing." Bini L., Heid H., Liberatori S., Geier G., Pallini V., Zwilling R. Electrophoresis 18:557-562(1997) [PubMed: 9150941] [Abstract] Cited for: PROTEIN SEQUENCE OF 56-65. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-321, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081559 Genomic DNA. Translation: CCD72415.1.
PIR	T33383.
RefSeq	NP_509142.1. NM_076741.4.
UniGene	Cel.17775.
3D structure databases
ProteinModelPortal	P55956.
SMR	P55956. Positions 18-395.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26574N.
IntAct	P55956. 1 interaction.
MINT	MINT-1064632.
STRING	P55956.
Protein family/group databases
MEROPS	A01.A69.
2D gel databases
Siena-2DPAGE	P55956.
Proteomic databases
PRIDE	P55956.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	H22K11.1; H22K11.1; H22K11.1.
GeneID	180947.
KEGG	cel:H22K11.1.
NMPDR	fig|6239.3.peg.23623.
UCSC	H22K11.1. c. elegans.
Organism-specific databases
CTD	180947.
WormBase	H22K11.1; CE19495; WBGene00000216; asp-3.
Phylogenomic databases
eggNOG	meNOG05119.
GeneTree	EMGT00050000005383.
HOGENOM	HBG590923.
InParanoid	P55956.
OMA	FYSVFDH.
PhylomeDB	P55956.
Gene expression databases
ArrayExpress	P55956.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
KO	K01386.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	911692.

Entry information

Entry name

ASP3_CAEEL

Accession

Primary (citable) accession number: P55956
Secondary accession number(s): Q9TXI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 20, 2002
Last modified:	December 14, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project