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P55917 (CXA8_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gap junction alpha-8 protein
Alternative name(s):
Connexin-49
Short name=Cx49
Lens fiber protein MP70
MP38
MP64
Gene names
Name:GJA8
OrganismOvis aries (Sheep) [Reference proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subunit structure

A connexon is composed of a hexamer of connexins.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctiongap junction.

Tissue specificity

Eye lens.

Sequence similarities

Belongs to the connexin family. Alpha-type (group II) subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 440439Gap junction alpha-8 protein
PRO_0000057832

Regions

Topological domain2 – 2322Cytoplasmic Potential
Transmembrane24 – 4623Helical; Potential
Topological domain47 – 7428Extracellular Potential
Transmembrane75 – 9723Helical; Potential
Topological domain98 – 15760Cytoplasmic Potential
Transmembrane158 – 18023Helical; Potential
Topological domain181 – 20929Extracellular Potential
Transmembrane210 – 22920Helical; Potential
Topological domain230 – 440211Cytoplasmic Potential

Natural variations

Natural variant91N → R.
Natural variant101I → L.
Natural variant141V → A.

Experimental info

Sequence conflict131E → N AA sequence Ref.3
Sequence conflict151N → Q AA sequence Ref.3
Sequence conflict421E → D in AAF01367. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P55917 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: BE6E478B98D83891

FASTA44049,160
        10         20         30         40         50         60 
MGDWSFLGNI LEEVNEHSTV IGRVWLTVLF IFRILILGTA AEFVWGDEQS DFVCNTQQPG 

        70         80         90        100        110        120 
CENVCYDEAF PISHIRLWVL QIIFVSTPSL VYVGHAVHHV RMEEKRKERE AEELSQQSPG 

       130        140        150        160        170        180 
NGGERAPLAA DQGSVKKSSS SSKGTKKFRL EGTLLRTYVC HIIFKTLFEV GFIVGHYFLY 

       190        200        210        220        230        240 
GFRILPLYRC SRWPCPNVVD CFVSRPTEKT IFILFMLSVA SVSLFLNILE MSHLGLKKIR 

       250        260        270        280        290        300 
SAFKRPVEQP LGEIPEKSLH SIAVSSIQKA KGYQLLEEEK IVSHYFPLTE VGMVEASPLS 

       310        320        330        340        350        360 
AKPFSQFEEK VGPGPLGDLS RAYQETLPSY AQVGAQEGVE EEQPIEAAAE PEVGDKSQEA 

       370        380        390        400        410        420 
ERVSTEGEET LAVLEEEKVE PPEVEKEAEK EETPPEKVSK QELTPEKAPS LCAELPGEDT 

       430        440 
RPLSRLSKAS SRARSDDLTV 

« Hide

References

[1]"Molecular cloning of sheep connexin49 and its identity with MP70."
Yang D.-I., Louis C.F.
Curr. Eye Res. 15:307-314(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Yang D.-I., Louis C.F.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Homologies between gap junction proteins in lens, heart and liver."
Kistler J., Christie D., Bullivant S.
Nature 331:721-723(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-21.
Tissue: Lens.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177913 Genomic DNA. Translation: AAF01367.1.
RefSeqNP_001123510.1. NM_001130038.1.
UniGeneOar.17419.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100170231.

Organism-specific databases

CTD2703.

Phylogenomic databases

HOVERGENHBG009576.

Family and domain databases

InterProIPR000500. Connexin.
IPR002266. Connexin50.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERPTHR11984. PTHR11984. 1 hit.
PfamPF00029. Connexin. 1 hit.
PF03509. Connexin50. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSPR00206. CONNEXIN.
PR01137. CONNEXINA8.
SMARTSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCXA8_SHEEP
AccessionPrimary (citable) accession number: P55917
Secondary accession number(s): Q9MYL3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families