P55917 (CXA8_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction alpha-8 protein

Alternative name(s):
Connexin-49
Short name=Cx49
Lens fiber protein MP70
MP38
MP64

Gene names

Name:

GJA8

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Eye lens.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell communication Inferred from electronic annotation. Source: InterPro
Cellular_component	connexon complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 440	439	Gap junction alpha-8 protein		PRO_0000057832
Regions
	Topological domain	2 – 23	22	Cytoplasmic Potential
	Transmembrane	24 – 46	23	Helical; Potential
	Topological domain	47 – 74	28	Extracellular Potential
	Transmembrane	75 – 97	23	Helical; Potential
	Topological domain	98 – 157	60	Cytoplasmic Potential
	Transmembrane	158 – 180	23	Helical; Potential
	Topological domain	181 – 209	29	Extracellular Potential
	Transmembrane	210 – 229	20	Helical; Potential
	Topological domain	230 – 440	211	Cytoplasmic Potential
Natural variations
	Natural variant	9	1	N → R.
	Natural variant	10	1	I → L.
	Natural variant	14	1	V → A.
Experimental info
	Sequence conflict	13	1	E → N AA sequence Ref.3
	Sequence conflict	15	1	N → Q AA sequence Ref.3
	Sequence conflict	42	1	E → D in AAF01367. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55917 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: BE6E478B98D83891	FASTA	440	49,160
	10 20 30 40 50 60 MGDWSFLGNI LEEVNEHSTV IGRVWLTVLF IFRILILGTA AEFVWGDEQS DFVCNTQQPG 70 80 90 100 110 120 CENVCYDEAF PISHIRLWVL QIIFVSTPSL VYVGHAVHHV RMEEKRKERE AEELSQQSPG 130 140 150 160 170 180 NGGERAPLAA DQGSVKKSSS SSKGTKKFRL EGTLLRTYVC HIIFKTLFEV GFIVGHYFLY 190 200 210 220 230 240 GFRILPLYRC SRWPCPNVVD CFVSRPTEKT IFILFMLSVA SVSLFLNILE MSHLGLKKIR 250 260 270 280 290 300 SAFKRPVEQP LGEIPEKSLH SIAVSSIQKA KGYQLLEEEK IVSHYFPLTE VGMVEASPLS 310 320 330 340 350 360 AKPFSQFEEK VGPGPLGDLS RAYQETLPSY AQVGAQEGVE EEQPIEAAAE PEVGDKSQEA 370 380 390 400 410 420 ERVSTEGEET LAVLEEEKVE PPEVEKEAEK EETPPEKVSK QELTPEKAPS LCAELPGEDT 430 440 RPLSRLSKAS SRARSDDLTV

References

[1]	"Molecular cloning of sheep connexin49 and its identity with MP70." Yang D.-I., Louis C.F. Curr. Eye Res. 15:307-314(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Yang D.-I., Louis C.F. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Homologies between gap junction proteins in lens, heart and liver." Kistler J., Christie D., Bullivant S. Nature 331:721-723(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Tissue: Lens.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177913 Genomic DNA. Translation: AAF01367.1.
RefSeq	NP_001123510.1. NM_001130038.1.
UniGene	Oar.17419.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100170231.
Organism-specific databases
CTD	2703.
Phylogenomic databases
HOVERGEN	HBG009576.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002266. Connexin50. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF03509. Connexin50. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01137. CONNEXINA8.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CXA8_SHEEP

Accession

Primary (citable) accession number: P55917
Secondary accession number(s): Q9MYL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program