Reviewed, UniProtKB/Swiss-Prot P55917 (CXA8_SHEEP)

Names and origin

Protein names

Recommended name:
    Gap junction alpha-8 protein
Alternative name(s):
    Connexin-49
      Short name=Cx49
    Lens fiber protein MP70
    MP64
    MP38

Gene names

Name:

GJA8

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Eye lens.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro
Cellular component	connexon complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 440	439	Gap junction alpha-8 protein		PRO_0000057832
Regions
	Topological domain	2 – 23	22	Cytoplasmic Potential
	Transmembrane	24 – 46	23	Potential
	Topological domain	47 – 74	28	Extracellular Potential
	Transmembrane	75 – 97	23	Potential
	Topological domain	98 – 157	60	Cytoplasmic Potential
	Transmembrane	158 – 180	23	Potential
	Topological domain	181 – 209	29	Extracellular Potential
	Transmembrane	210 – 229	20	Potential
	Topological domain	230 – 440	211	Cytoplasmic Potential
Natural variations
	Natural variant	9	1	N → R
	Natural variant	10	1	I → L
	Natural variant	14	1	V → A
Experimental info
	Sequence conflict	13	1	E → N AA sequence Ref.3
	Sequence conflict	15	1	N → Q AA sequence Ref.3
	Sequence conflict	42	1	E → D in AAF01367. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55917-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: BE6E478B98D83891	FASTA	440	49,160
	10 20 30 40 50 60 MGDWSFLGNI LEEVNEHSTV IGRVWLTVLF IFRILILGTA AEFVWGDEQS DFVCNTQQPG 70 80 90 100 110 120 CENVCYDEAF PISHIRLWVL QIIFVSTPSL VYVGHAVHHV RMEEKRKERE AEELSQQSPG 130 140 150 160 170 180 NGGERAPLAA DQGSVKKSSS SSKGTKKFRL EGTLLRTYVC HIIFKTLFEV GFIVGHYFLY 190 200 210 220 230 240 GFRILPLYRC SRWPCPNVVD CFVSRPTEKT IFILFMLSVA SVSLFLNILE MSHLGLKKIR 250 260 270 280 290 300 SAFKRPVEQP LGEIPEKSLH SIAVSSIQKA KGYQLLEEEK IVSHYFPLTE VGMVEASPLS 310 320 330 340 350 360 AKPFSQFEEK VGPGPLGDLS RAYQETLPSY AQVGAQEGVE EEQPIEAAAE PEVGDKSQEA 370 380 390 400 410 420 ERVSTEGEET LAVLEEEKVE PPEVEKEAEK EETPPEKVSK QELTPEKAPS LCAELPGEDT 430 440 RPLSRLSKAS SRARSDDLTV

References

[1]	"Molecular cloning of sheep connexin49 and its identity with MP70." Yang D.-I., Louis C.F. Curr. Eye Res. 15:307-314(1996) [PubMed: 8654111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Yang D.-I., Louis C.F. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Homologies between gap junction proteins in lens, heart and liver." Kistler J., Christie D., Bullivant S. Nature 331:721-723(1988) [PubMed: 2830542] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Tissue: Lens.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF177913 Genomic DNA. Translation: AAF01367.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P55917.
Family and domain databases
InterPro	IPR000500. Connexin. IPR013125. Connexin50_C. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. Connexin. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF03509. Connexin50. 1 hit. [Graphical view]
SMART	SM00037. CNX. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CXA8_SHEEP

Accession

Primary (citable) accession number: P55917
Secondary accession number(s): Q9MYL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)