Reviewed, UniProtKB/Swiss-Prot P55913 (THII_SALTY)

Names and origin

Protein names

Recommended name:
    Thiamine biosynthesis protein thiI

Gene names

Name:	thiI
Ordered Locus Names:	STM0425

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for the synthesis of the thiazole moiety. HAMAP MF_00021
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP MF_00021
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiI family. Contains 1 rhodanese domain. Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Cellular component	Cytoplasm
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Thiamine biosynthesis protein thiI HAMAP MF_00021		PRO_0000154860
Regions
	Domain	61 – 165	105	THUMP
	Domain	404 – 482	79	Rhodanese
Sites
	Active site	456	1	Cysteine persulfide intermediate By similarity
Experimental info
	Sequence conflict	365	1	E → R in AAB39648. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P55913-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 41CC9EE3740CE5D2	FASTA	482	54,831
	10 20 30 40 50 60 MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI EVRAKDENQR 70 80 90 100 110 120 LVIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALAQYRE QLEGKTFCVR VKRRGKHEFS 130 140 150 160 170 180 SIEVERYVGG GLNQHIESAR VKLTNPDVTV HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV 190 200 210 220 230 240 LSLISGGFDS GVSSYMLMRR GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV 250 260 270 280 290 300 AINFEPVVGE ILEKVDDGQM GVVLKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN 310 320 330 340 350 360 LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS PTVKAIKAKI 370 380 390 400 410 420 EAEEENFDFS ILDKVVEEAN NVDIREIAQQ TQQEVVEVET VSGFGPNDVI LDIRSVDEQD 430 440 450 460 470 480 DKPLKVEGVD VVSLPFYKLS TKFGDLDQSK TWLLWCERGV MSRLQALYLR EQGFANVKVY RP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium." Webb E., Claas K., Downs D.M. J. Bacteriol. 179:4399-4402(1997) [PubMed: 9209060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	Metcalf W.W., Jiang W., Wanner B.L. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 365-482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U94901 Genomic DNA. Translation: AAB63031.1. AE006468 Genomic DNA. Translation: AAL19379.1. U69493 Genomic DNA. Translation: AAB39648.1.
PIR	T46944.
RefSeq	NP_459420.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P55913.
Genome annotation databases
GeneID	1251944.
GenomeReviews	Gene locus STM0425 in contig AE006468_GR.
KEGG	stm:STM0425.
NMPDR	fig|99287.1.peg.410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P55913.
OMA	GYPIGTQ
Enzyme and pathway databases
BioCyc	STYP99287:STM0425-MON.
Family and domain databases
HAMAP	MF_00021. [Tree]
InterPro	IPR001763. Rhodanese-like. IPR003720. ThiI. IPR020536. ThiI_C. IPR004114. THUMP. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
Pfam	PF02568. ThiI. 1 hit. PF02926. THUMP. 1 hit. [Graphical view]
TIGRFAMs	TIGR00342. ThiI. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS51165. THUMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THII_SALTY

Accession

Primary (citable) accession number: P55913
Secondary accession number(s): O06955

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	November 24, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)